FATC Domain Deletion Compromises ATM Protein Stability, Blocks Lymphocyte Development, and Promotes Lymphomagenesis

2021 ◽  
pp. ji2000967
Author(s):  
Maja Milanovic ◽  
Zhengping Shao ◽  
Verna M. Estes ◽  
Xiaobin S. Wang ◽  
Demis Menolfi ◽  
...  
Keyword(s):  
Protein Stability ◽  
Lymphocyte Development ◽  
Atm Protein ◽  
Fatc Domain

scholarly journals DNA Damage-Induced Acetylation of Lysine 3016 of ATM Activates ATM Kinase Activity

2007 ◽  
Vol 27 (24) ◽  
pp. 8502-8509 ◽  
Author(s):  
Yingli Sun ◽  
Ye Xu ◽  
Kanaklata Roy ◽  
Brendan D. Price
Keyword(s):  
Dna Damage ◽  
Kinase Activity ◽  
Histone Acetyltransferase ◽  
Kinase Domain ◽  
Atm Kinase ◽  
Lysine Residues ◽  
Atm Protein ◽  
Fatc Domain

ABSTRACT The ATM protein kinase is essential for cells to repair and survive genotoxic events. The activation of ATM's kinase activity involves acetylation of ATM by the Tip60 histone acetyltransferase. In this study, systematic mutagenesis of lysine residues was used to identify regulatory ATM acetylation sites. The results identify a single acetylation site at lysine 3016, which is located in the highly conserved C-terminal FATC domain adjacent to the kinase domain. Antibodies specific for acetyl-lysine 3016 demonstrate rapid (within 5 min) in vivo acetylation of ATM following exposure to bleomycin. Furthermore, lysine 3016 of ATM is a substrate in vitro for the Tip60 histone acetyltransferase. Mutation of lysine 3016 does not affect unstimulated ATM kinase activity but does abolish upregulation of ATM's kinase activity by DNA damage, inhibits the conversion of inactive ATM dimers to active ATM monomers, and prevents the ATM-dependent phosphorylation of the p53 and chk2 proteins. These results are consistent with a model in which acetylation of lysine 3016 in the FATC domain of ATM activates the kinase activity of ATM. The acetylation of ATM on lysine 3016 by Tip60 is therefore a key step linking the detection of DNA damage and the activation of ATM kinase activity.

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