scholarly journals Phenotypic and Ig Repertoire Analyses Indicate a Common Origin of IgD−CD27− Double Negative B Cells in Healthy Individuals and Multiple Sclerosis Patients

2019 ◽  
Vol 203 (6) ◽  
pp. 1650-1664 ◽  
Author(s):  
Judith Fraussen ◽  
Susanna Marquez ◽  
Kazushiro Takata ◽  
Lien Beckers ◽  
Gwendoline Montes Diaz ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
B Cells ◽  
Common Origin ◽  
Double Negative ◽  
Healthy Individuals ◽  
Multiple Sclerosis Patients

scholarly journals Rituximab reduces B cells and T cells in cerebrospinal fluid of multiple sclerosis patients

2006 ◽  
Vol 180 (1-2) ◽  
pp. 63-70 ◽  
Author(s):  
Anne H. Cross ◽  
Jennifer L. Stark ◽  
Joanne Lauber ◽  
Michael J. Ramsbottom ◽  
Jeri-Anne Lyons
Keyword(s):  
Multiple Sclerosis ◽  
T Cells ◽  
Cerebrospinal Fluid ◽  
B Cells ◽  
Multiple Sclerosis Patients

Multiple sclerosis patients have peripheral blood CD45RO+ B cells and increased intestinal permeability

1996 ◽  
Vol 41 (12) ◽  
pp. 2493-2498 ◽  
Author(s):  
Bruce Yacyshyn ◽  
Jon Meddings ◽  
Daniel Sadowski ◽  
Mary Beth Bowen-Yacyshyn
Keyword(s):  
Multiple Sclerosis ◽  
B Cells ◽  
Intestinal Permeability ◽  
Peripheral Blood ◽  
Multiple Sclerosis Patients

scholarly journals Association of CD58 polymorphism and multiple sclerosis in Malaysia: a pilot study

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Yee Ming Ching ◽  
Shanthi Viswanathan ◽  
Nurhanani Mohamed Nor ◽  
Shuwahida Shuib ◽  
Balqis Kamarudin ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Pilot Study ◽  
Gene Polymorphism ◽  
Age Of Onset ◽  
Nucleotide Polymorphisms ◽  
Healthy Individuals ◽  
Disease Manifestation ◽  
Southeast Asians ◽  
Multiple Sclerosis Patients ◽  
The Impact

Abstract Background Multiple sclerosis is an immune mediated disease targeting the central nervous system. Association of non-human leukocyte antigen gene, CD58, with multiple sclerosis has been reported in several populations but is unclear among Southeast Asians. This pilot study was conducted to explore the association between CD58 polymorphism and multiple sclerosis among the Malay population in Malaysia. Methods Blood samples were collected from 27 multiple sclerosis patients, and compared with 58 age- and gender matched healthy individuals. All patients were tested negative for anti-aquaporin 4. DNA was extracted from the blood and genotyped for 3 single nucleotide polymorphisms rs12044852, rs2300747 and rs1335532 of gene CD58 by real-time PCR. Results The majority of multiple sclerosis patients were female (85.2%). The general mean age of onset was 30.5 years. Genotyping results showed that frequencies of the alleles were between 40 and 50% for MS patients and healthy individuals. Association (allelic model) between multiple sclerosis and CD58 gene polymorphism alleles rs12044852 (p = 0.410), rs2300747 (p = 0.881) and rs1335532 (p = 0.407) were indistinct. Conclusions The impact of the CD58 gene polymorphism was not prominent in this pilot study, implying that genetic composition contributing to multiple sclerosis may be different between different populations, thus results in a heterogeneity of disease manifestation and distribution.

scholarly journals Immunomodulatory Potential of Human Mesenchymal Stem Cells and their Exosomes on Multiple Sclerosis

2021 ◽  
Author(s):  
Hussein Baharlooi ◽  
Zahra Salehi ◽  
Moein Minbashi Moeini ◽  
Nima Rezaei ◽  
Maryam Azimi
Keyword(s):  
Multiple Sclerosis ◽  
Stem Cells ◽  
T Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Interleukin 17 ◽  
Healthy Individuals ◽  
Healthy Control ◽  
Multiple Sclerosis Patients

Purpose: Promising advances have been made in mesenchymal stem cell transplantation to re-induce the immune tolerance in neuroinflammatory animal models and Multiple Sclerosis patients. The available evidence demonstrated that immunomodulatory effects of mesenchymal stem cell are particularly exerted through releasing exosomes to their environment. We therefore, aimed to comparatively assess the potential effect of mesenchymal stem cells and mesenchymal stem cells-derived exosomes on proliferation and function of the CD4+CD25− conventional T cells, isolated from relapsing-remitting Multiple Sclerosis patients. Methods: Mesenchymal stem cells were isolated from human umbilical cord tissues and used for exosome isolation via ultracentrifugation. Both mesenchymal stem cells and mesenchymal stem cells-derived exosomes were evaluated for their anti-inflammatory effects against the proliferation of T cells isolated from two groups of individuals in vitro, MS patients and healthy subjects. Cytokine production of conventional T cells (interferon-γ, interleukin-10, and interleukin-17) was also assessed, using flow cytometry for the patients and healthy individuals. Results: Here, evidence shows that MSCs and MSC-derived exosomes dampen proliferation and percentage of conventional T cells that produce IFN-γ (healthy control: p<0.001) and interleukin-17 (healthy control: p<0.001, MS patients: p<0.001), with a significant increase of IL-10 producing cells in the patients and healthy individuals. Surprisingly, MSC-derived exosomes demonstrated higher immune-modulating properties on conventional T cells responses, compared to MSCs. Conclusion: The current study, provides a novel approach of exocytosis on autoimmune therapy. In particular, Mesenchymal stem cell -derived exosomes, which are cell-derived biologics, could be considered as an alternative for Mesenchymal stem cells in treating multiple sclerosis.

Identification of peptides binding to IgG in the CSF of Multiple Sclerosis patients

1998 ◽  
Vol 4 (1) ◽  
pp. 31-36 ◽  
Author(s):  
I Cortese ◽  
S Capone ◽  
R Tafi ◽  
L ME Grimaldi ◽  
A Nicosia ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
General Population ◽  
Peptide Libraries ◽  
Healthy Individuals ◽  
Random Peptide ◽  
Random Peptide Libraries ◽  
Multiple Sclerosis Patients

Phage displayed random peptide libraries were screened in order to identify phagotopes reacting with the IgG present in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients. Six families of phagotopes each composed of different isolates were identified. These phagotopes were used as reagents, to characterise IgG present in the CSF of MS patients. The following results were obtained: (a) CSF antibodies from different patients display different specificities; (b) anti-phagotope antibodies are also present in the serum of MS patients; (c) Anti-phagotope antibodies are equally frequent in the serum of MS patients and of healthy individuals; (d) some of the anti-phagotope antibodies are enriched in the CSF of MS patients. These data show that the natural antigen(s) recognised by CSF antibodies is rather common in the general population. By using the selected phagotopes as immunogens in rabbits, we have derived large quantities of anti-phagotope antisera which can provide for useful tools toward the identification of the natural antigen(s) recognised by MS CSF antibodies.

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