scholarly journals Anaphylactic Release of Mucosal Mast Cell Granule Proteases: Role of Serpins in the Differential Clearance of Mouse Mast Cell Proteases-1 and -2

2006 ◽  
Vol 176 (2) ◽  
pp. 899-904 ◽  
Author(s):  
Alan D. Pemberton ◽  
Steven H. Wright ◽  
Pamela A. Knight ◽  
Hugh R. P. Miller
Keyword(s):  
Mast Cell ◽  
Cell Granule ◽  
Mucosal Mast Cell ◽  
Mast Cell Granule ◽  
Mouse Mast Cell

scholarly journals Release of the mucosal mast cell granule chymase, rat mast cell protease-II, during anaphylaxis is associated with the rapid development of paracellular permeability to macromolecules in rat jejunum.

1995 ◽  
Vol 182 (6) ◽  
pp. 1871-1881 ◽  
Author(s):  
C L Scudamore ◽  
E M Thornton ◽  
L McMillan ◽  
G F Newlands ◽  
H R Miller
Keyword(s):  
Mast Cell ◽  
Mesenteric Artery ◽  
Ex Vivo ◽  
Rapid Development ◽  
Cell Granule ◽  
Histological Evaluation ◽  
Mucosal Mast Cell ◽  
Type I ◽  
Mast Cell Protease ◽  
Mast Cell Granule

The soluble granule chymase, rat mast cell protease-II (RMCP-II), is abundantly expressed in intestinal mucosal mast cells (MMC) but its function is not known. One hypothesis is that RMCP-II degrades the epithelial basement membrane and promotes the loss of enterocytes typically associated with type I hypersensitivity reactions in the rat. To test this hypothesis more directly, ex vivo perfusion of the cranial mesenteric artery and jejunal lumen was used to monitor the anaphylactic release of RMCP-II and its effects on mucosal permeability and epithelial integrity. Within 2 min of intravascular challenge with soluble adult Nippostrongylus brasiliensis worm antigen there was a 1,000-fold (P < 0.02) increase in the concentration of RMCP-II in the vascular perfusate from the jejunum of Nippostrongylus-sensitized rats but not the controls. Similarly, translocation of RMCP-II into the gut lumen increased 10-fold (P < 0.02) after 2 min only in worm antigen-challenged immune rats. Using an identical protocol, but incorporating Evans blue-labeled human serum albumin (EB-HSA) in the vascular perfusate, the timing of the release of RMCP-II into the two compartments was very similar to the first experiment and furthermore the translocation of EB-HSA increased 18-fold (P < 0.05) after 4 min in sensitized rats challenged with worm antigen. To examine the effects of RMCP-II more directly 1 mg of the highly purified chymase was introduced into the cranial mesenteric artery in ex vivo perfused normal rats. A significant (P < 0.05) 70-fold increase in concentration of RMCP-II in jejunal perfusate occurred after 6 min. In a repeat dose-response experiment, infusion of 0.375, 0.75, or 1.5 mg of RMCP-II, together with EB-HSA, established that the cumulative amounts of RMCP-II and EB-HSA translocated from the vasculature to the gut lumen in each perfusion (during the 10-min period of RMCP-II infusion) were significantly correlated. Analysis of intestinal perfusates by SDS-PAGE and by Western blotting using monoclonal anti-RMCP-II antibody confirmed that there was a concomitant translocation of both the protease and EB-HSA into the gut lumen. Histological evaluation of the mucosa failed to reveal any significant morphological change in any of the experiments. The rapid development of macromolecular leak, its association with the translocation of RMCP-II, and the absence of gross epithelial lesions, suggest for the first time that a mast cell granule chymase increases epithelial permeability via a paracellular route and implies that the substrate may be a protein, or proteins, in the epithelial junctional complex.

Expression, Function and Regulation of Mast Cell Granule Chymases During Mucosal Allergic Responses

2000 ◽  
pp. 257-273 ◽  
Author(s):  
Pamela A. Knight ◽  
Steven H. Wright ◽  
Elisabeth M. Thornton ◽  
Jeremy Brown ◽  
Hugh R.P. Miller
Keyword(s):  
Mast Cell ◽  
Cell Granule ◽  
Mast Cell Granule

Constitutive secretion of the granule chymase mouse mast cell protease-1 and the chemokine, CCL2, by mucosal mast cell homologues

2003 ◽  
Vol 33 (1) ◽  
pp. 132-146 ◽  
Author(s):  
J. K. Brown ◽  
P. A. Knight ◽  
S. H. Wright ◽  
E. M. Thornton ◽  
H. R. P. Miller
Keyword(s):  
Mast Cell ◽  
Mucosal Mast Cell ◽  
Mast Cell Protease ◽  
Constitutive Secretion ◽  
Mouse Mast Cell

scholarly journals Mucosal Defense against Gastrointestinal Nematodes: Responses of Mucosal Mast Cells and Mouse Mast Cell Protease 1 during PrimaryStrongyloides venezuelensis Infection in FcRγ-Knockout Mice

2000 ◽  
Vol 68 (9) ◽  
pp. 4968-4971 ◽  
Author(s):  
Denis N. Onah ◽  
Fukumi Uchiyama ◽  
Yuuko Nagakui ◽  
Masao Ono ◽  
Toshiyuki Takai ◽  
...  
Keyword(s):  
Mast Cell ◽  
Gastrointestinal Nematodes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mucosal Mast Cell ◽  
Mast Cell Protease ◽  
Intestinal Nematode ◽  
Nematode Parasites ◽  
Cell Counts ◽  
Strongyloides Venezuelensis ◽  
Mouse Mast Cell

ABSTRACT A possible role for the γ subunit of immunoglobulin Fc receptors (FcR) in mucosal defenses against intestinal nematode parasites was studied using age-matched FcRγ-knockout (FcRγ−/−) and wild-type (FcRγ+/+) C57BL/6 mice. Mice were infected subcutaneously with 3,000 infective larvae of Strongyloides venezuelensis, and the degree of infection was monitored by daily fecal egg counts and adult worm recovery on days 8 and 13 postinfection. Mucosal mast cell (MMC) responses were assayed by in situ intestinal mast cell counts in stained histological sections of the jejunum and by measuring mouse mast cell protease 1 (MMCP-1) release in serum using sandwich enzyme-linked immunosorbent assay. FcRγ−/− mice had significantly higher egg counts (P < 0.01) and numbers of adult worms (P < 0.05) than FcRγ+/+mice, but mastocytosis and serum MMCP-1 release were comparable. It was concluded that MMCP-1 release may be spontaneous, does not depend on mast cell degranulation via the FcRγ signaling system, and appears to play no role in the expulsion of S. venezuelensis. The delay in worm expulsion in the FcRγ−/− mice might be related to inability of the MMC to degranulate and release effector molecules other than MMCP-1, since FcRγ deletion abrogates mast cell degranulative responses.

Extracellular Localization of Human Connective Tissue Mast Cell Granule Contents

1990 ◽  
Vol 96 (6) ◽  
pp. 857-863 ◽  
Author(s):  
Michael S. Kaminer ◽  
Robert M. Lavker ◽  
Laurence J. Walsh ◽  
Diana. Whitaker ◽  
Burton. Zweiman
Keyword(s):  
Mast Cell ◽  
Connective Tissue ◽  
Cell Granule ◽  
Tissue Mast Cell ◽  
Mast Cell Granule

scholarly journals Pivotal Role of Mouse Mast Cell Protease 4 in the Conversion and Pressor Properties of Big-Endothelin-1

2013 ◽  
Vol 346 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Martin Houde ◽  
Marc-David Jamain ◽  
Julie Labonté ◽  
Louisane Desbiens ◽  
Gunnar Pejler ◽  
...  
Keyword(s):  
Mast Cell ◽  
Pivotal Role ◽  
Endothelin 1 ◽  
Mast Cell Protease ◽  
Mouse Mast Cell
Sign in / Sign up

Export Citation Format

Share Document