scholarly journals Rapid and Efficient Production of Human Functional Mast Cells through a Three-Dimensional Culture of Adipose Tissue–Derived Stromal Vascular Cells

2018 ◽  
Vol 201 (12) ◽  
pp. 3815-3821 ◽  
Author(s):  
Jenny Paupert ◽  
Eric Espinosa ◽  
Nicolas Cenac ◽  
Virginie Robert ◽  
Patrick Laharrague ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Mast Cells ◽  
Three Dimensional ◽  
Efficient Production ◽  
Vascular Cells ◽  
Three Dimensional Culture

Three-dimensional culture model for analyzing crosstalk between adipose tissue and hepatocytes

2013 ◽  
Vol 352 (3) ◽  
pp. 611-621 ◽  
Author(s):  
Aki Nishijima-Matsunobu ◽  
Shigehisa Aoki ◽  
Kazuyoshi Uchihashi ◽  
Kazuma Fujimoto ◽  
Shuji Toda
Keyword(s):  
Adipose Tissue ◽  
Three Dimensional ◽  
Culture Model ◽  
Three Dimensional Culture

scholarly journals Paracrine study of adipose tissue-derived mesenchymal stem cells (ADMSCs) in a self-assembling nano-polypeptide hydrogel environment

2021 ◽  
Vol 10 (1) ◽  
pp. 547-554
Author(s):  
Jianmin Ling ◽  
Ailing Tian ◽  
Xin Yi ◽  
Nianfeng Sun
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Western Blotting ◽  
Three Dimensional ◽  
Culture Conditions ◽  
High Expression ◽  
Expression Levels ◽  
Self Assembling ◽  
Three Dimensional Culture

Abstract To research the paracrine role of adipose tissue-derived mesenchymal stem cells (ADMSCs) in promoting angiogenesis under the three-dimensional culture conditions consisting of a functionalized self-assembling peptide nanofiber hydrogel. ADMSCs were isolated, extracted, and then identified. Three kinds of peptides (RADAI-16, RGD, and KLT) were prepared, and a functionalized self-assembling peptide nanofiber hydrogel was produced by mixing RADAI-16, RGD, and KLT in a volume ratio 2:1:1. AFM was used to observe RADAI-16, RGD, KLT, and the functionalized self-assembling peptide nanofiber hydrogel. Then, ADMSCs were cultured under three-dimensional conditions consisting of the peptide nanofiber hydrogel, and AFM was used to observe cell migration. The ADMSCs in the common culture group (37°C, 5% CO2 cell culture box) and hypoxic culture group (37°C, 10% CO2, and 1% O2 hypoxic culture box) acted as controls. ADMSCs were three-dimensionally cultured in situ for 1 day, and then the concentrations of HGF and VEGF in the supernatant were determined by ELISA. Cells were extracted from the peptide nanofiber hydrogel, and HO-1 expression was detected by western blotting. ADMSCs have high expression levels of CD29, CD90, and CDl05 and low expression levels of CD34 and CD45. In addition, they can differentiate into adipocytes and osteocytes. The diameters of the fibers of RADAI-16, RGD, KLT, and the functionalized self-assembling peptide hydrogel are 17.34 ± 1.82, 15.50 ± 1.41, 13.77 ± 1.18, and 20.26 ± 1.25 nm, respectively. AFM indicated that cells in the functionalized self-assembling peptide nanofiber hydrogel migrated farther than those in RADAI-16. The concentrations of HGF under common, hypoxic, and three-dimensional culture conditions were 47.31 ± 6.75, 247.86 ± 17.59, and 297.25 ± 17.95 pg/mL, respectively, while the concentrations of VEGF were 218.30 ± 3.03, 267.13 ± 4.27, and 289.14 ± 3.11 pg/mL, respectively. Both HGF and VEGF were expressed more in the presence of the functionalized self-assembling peptide nanofiber hydrogel than in its absence (P < 0.05). Using western blotting, ADMSCs cultured under hypoxic and three-dimensional conditions were found to have high expression levels of HO-1. Culturing ADMSCs under three-dimensional conditions consisting of functionalized self-assembling peptide nanofiber hydrogels can promote their paracrine role in angiogenesis, such as HGF and VEGF, and hypoxia is one of the important elements.

Thrombin Augments Vascular Cell-dependent Migration of Human Mast Cells: Role of MGF

1997 ◽  
Vol 77 (03) ◽  
pp. 577-584 ◽  
Author(s):  
Mehrdad Baghestanian ◽  
Roland Hofbauer ◽  
Hans G Kress ◽  
Johann Wojta ◽  
Astrid Fabry ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Mast Cells ◽  
Umbilical Vein ◽  
Vascular Cells ◽  
Migratory Response ◽  
Thrombin Activation ◽  
Umbilical Vein Endothelial Cells ◽  
Primary Tissue ◽  
Local Expression

SummaryRecent data suggest that auricular thrombosis is associated with accumulation of mast cells (MC) in the upper endocardium (where usually no MC reside) and local expression of MGF (mast cell growth factor) (25). In this study, the role of vascular cells, thrombin-activation and MGF, in MC-migration was analyzed. For this purpose, cultured human auricular endocardial cells (HAUEC), umbilical vein endothelial cells (HUVEC) and uterine-(HUTMEC) and skin-derived (HSMEC) microvascular endothelial cells were exposed to thrombin or control medium, and the migration of primary tissue MC (lung, n = 6) and HMC-1 cells (human MC-line) against vascular cells (supernatants) measured. Supernatants (24 h) of unstimulated vascular cells (monolayers of endocardium or endothelium) as well as recombinant (rh) MGF induced a significant migratory response in HMC-1 (control: 3025 ± 344 cells [100 ± 11.4%] vs. MGF, 100 ng/ml: 8806 ± 1019 [291 ± 34%] vs. HAUEC: 9703 ± 1506 [320.8 ± 49.8%] vs. HUTMEC: 8950 ± 1857 [295.9 ± 61.4%] vs. HSMEC: 9965 ± 2018 [329.4 ± 66.7%] vs. HUVEC: 9487 ± 1402 [313.6 ± 46.4%], p <0.05) as well as in primary lung MC. Thrombin-activation (5 U/ml, 12 h) of vascular cells led to an augmentation of the directed migration of MC as well as to a hirudin-sensitive increase in MGF synthesis and release. Moreover, a blocking anti-MGF antibody was found to inhibit MC-migration induced by unstimulated or thrombin-activated vascular cells. Together, these data show that endocardial and other vascular cells can induce migration of human MC. This MC-chemotactic signal of the vasculature is associated with expression and release of MGF, augmentable by thrombin, and may play a role in the pathophysiology of (auricular) thrombosis.

scholarly journals Isolation and culture of human endometrial cells in a three-dimensional culture system

Reproduction ◽  
1994 ◽  
Vol 101 (2) ◽  
pp. 327-332 ◽  
Author(s):  
U. Bentin-Ley ◽  
B. Pedersen ◽  
S. Lindenberg ◽  
J. F. Larsen ◽  
L. Hamberger ◽  
...  
Keyword(s):  
Culture System ◽  
Three Dimensional ◽  
Endometrial Cells ◽  
Three Dimensional Culture

Effects of Three-Dimensional Culture and Growth Factors on the Chondrogenic Differentiation of Murine Embryonic Stem Cells

Stem Cells ◽  
2006 ◽  
Vol 24 (2) ◽  
pp. 284-291 ◽  
Author(s):  
Nathaniel S. Hwang ◽  
Myoung Sook Kim ◽  
Somponnat Sampattavanich ◽  
Jin Hyen Baek ◽  
Zijun Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Embryonic Stem Cells ◽  
Chondrogenic Differentiation ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Three Dimensional Culture

Porcine spermatogonial stem cells self-renew effectively in a three dimensional culture microenvironment

2017 ◽  
Vol 41 (12) ◽  
pp. 1316-1324 ◽  
Author(s):  
Ji Eun Park ◽  
Min Hee Park ◽  
Min Seong Kim ◽  
Yeo Reum Park ◽  
Jung Im Yun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Three Dimensional ◽  
Spermatogonial Stem Cells ◽  
Three Dimensional Culture
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