scholarly journals Complexity of Complement Resistance Factors Expressed byAcinetobacter baumanniiNeeded for Survival in Human Serum

2017 ◽  
Vol 199 (8) ◽  
pp. 2803-2814 ◽  
Author(s):  
Amaro F. Sanchez-Larrayoz ◽  
Noha M. Elhosseiny ◽  
Marc G. Chevrette ◽  
Yang Fu ◽  
Peter Giunta ◽  
...  
Keyword(s):  
Human Serum ◽  
Resistance Factors ◽  
Complement Resistance ◽  
Complement Resistance Factors

scholarly journals Contribution of the Infection-Associated Complement Regulator-Acquiring Surface Protein 4 (ErpC) to Complement Resistance ofBorrelia burgdorferi

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Claudia Hammerschmidt ◽  
Teresia Hallström ◽  
Christine Skerka ◽  
Reinhard Wallich ◽  
Brian Stevenson ◽  
...  
Keyword(s):  
Human Serum ◽  
Magnetic Beads ◽  
Surface Protein ◽  
Factor H ◽  
Surface Proteins ◽  
Complement Components ◽  
Complement Resistance ◽  
Complement Regulators ◽  
Complement Regulator ◽  
The Impact

Borrelia burgdorferievades complement-mediated killing by interacting with complement regulators through distinct complement regulator-acquiring surface proteins (CRASPs). Here, we extend our analyses to the contribution of CRASP-4 in mediating complement resistance ofB. burgdorferiand its interaction with human complement regulators. CRASP-4 (also known as ErpC) was immobilized onto magnetic beads and used to capture proteins from human serum. Following Western blotting, factor H (CFH), CFH-related protein 1 (CFHR1), CFHR2, and CFHR5 were identified as ligands of CRASP-4. To analyze the impact of native CRASP-4 on mediating survival of serum-sensitive cells in human serum, aB. gariniistrain was generated that ectopically expresses CRASP-4. CRASP-4-producing bacteria bound CFHR1, CFHR2, and CFHR5 but not CFH. In addition, transformed spirochetes deposited significant amounts of lethal complement components on their surface and were susceptible to human serum, thus indicating that CRASP-4 plays a subordinate role in complement resistance ofB. burgdorferi.

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