scholarly journals Role of Sulfation in CD44-Mediated Hyaluronan Binding Induced by Inflammatory Mediators in Human CD14+ Peripheral Blood Monocytes

2001 ◽  
Vol 167 (9) ◽  
pp. 5367-5374 ◽  
Author(s):  
Kelly L. Brown ◽  
Arpita Maiti ◽  
Pauline Johnson
Keyword(s):  
Inflammatory Mediators ◽  
Peripheral Blood ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Hyaluronan Binding

scholarly journals AP-1 (Activated Protein-1) Transcription Factor JunD Regulates Ischemia/Reperfusion Brain Damage via IL-1β (Interleukin-1β)

Stroke ◽  
2019 ◽  
Vol 50 (2) ◽  
pp. 469-477 ◽  
Author(s):  
Candela Diaz-Cañestro ◽  
Martin F. Reiner ◽  
Nicole R. Bonetti ◽  
Luca Liberale ◽  
Mario Merlini ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Brain Injury ◽  
Acute Ischemic Stroke ◽  
Peripheral Blood ◽  
Ischemia Reperfusion ◽  
Interleukin 1Β ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes

Background and Purpose— Inflammation is a major pathogenic component of ischemia/reperfusion brain injury, and as such, interventions aimed at inhibiting inflammatory mediators promise to be effective strategies in stroke therapy. JunD—a member of the AP-1 (activated protein-1) family of transcription factors—was recently shown to regulate inflammation by targeting IL (interleukin)-1β synthesis and macrophage activation. The purpose of the present study was to assess the role of JunD in ischemia/reperfusion-induced brain injury. Methods— WT (wild type) mice randomly treated with either JunD or scramble (control) siRNA were subjected to 45 minutes of transient middle cerebral artery occlusion followed by 24 hours of reperfusion. Stroke size, neurological deficit, plasma/brain cytokines, and oxidative stress determined by 4-hydroxynonenal immunofluorescence staining were evaluated 24 hours after reperfusion. Additionally, the role of IL-1β was investigated by treating JunD siRNA mice with an anti–IL-1β monoclonal antibody on reperfusion. Finally, JunD expression was assessed in peripheral blood monocytes isolated from patients with acute ischemic stroke. Results— In vivo JunD knockdown resulted in increased stroke size, reduced neurological function, and increased systemic inflammation, as confirmed by higher neutrophil count and lymphopenia. Brain tissue IL-1β levels were augmented in JunD siRNA mice as compared with scramble siRNA, whereas no difference was detected in IL-6, TNF-α (tumor necrosis factor-α), and 4-hydroxynonenal levels. The deleterious effects of silencing of JunD were rescued by treating mice with an anti–IL-1β antibody. In addition, JunD expression was decreased in peripheral blood monocytes of patients with acute ischemic stroke at 6 and 24 hours after onset of stroke symptoms compared with sex- and age-matched healthy controls. Conclusions— JunD blunts ischemia/reperfusion-induced brain injury via suppression of IL-1β.

Investigate the role of GPR15/BOB in the SLE patients

2018 ◽  
Vol 6 (1) ◽  
pp. 19-32
Author(s):  
Caroline G. Jackson ◽  
Donald R. Kwan
Keyword(s):  
Peripheral Blood ◽  
Messenger Rna ◽  
Viral Envelope ◽  
Orphan Receptor ◽  
Blood Monocytes ◽  
Rt Pcr ◽  
Peripheral Blood Monocytes ◽  
Healthy Donors ◽  
Hiv 1

GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. The objective of this study was to investigate the expression of orphan receptor GPR15/BOB in the serum of SLE patients and non-SLE healthy people. GPR15/BOB expression was analysed by flow cytometry while, GPR15/BOB messenger RNA was examined in peripheral blood monocytes by RT-PCR. GPR15/BOB mRNA was detected in all periphral blood of SLE patients examined. Further, a significant increase in GPR15/BOB expression as measured by mean fluorescence intensity was observed on SLE PB neutrophils compared to these cell populations from healthy donors. We concluded that GPR15/BOB is expressed in monocytes and neutrophils in peripheral blood, and expression is up-regulated in SLE patients compared to controls. GPR15/BOB may play a role in SLE pathogenesis.

scholarly journals e0681 Effect of chemotatic factor FKN on NF- B and TNF-  expression in peripheral blood monocytes and the role of PI3K

Heart ◽  
2010 ◽  
Vol 96 (Suppl 3) ◽  
pp. A211-A211
Author(s):  
S. Jian ◽  
G. Hui-jiao ◽  
L. Ming-ming ◽  
H. Wen-li ◽  
Y. Chun-yan ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes

The role of nonspecific immune protection in the development of disease in workers GUP «Moscow metro»

2020 ◽  
pp. 798-799
Author(s):  
E. S. Tsidilkovskaya ◽  
A. S. Likontseva ◽  
A. V. Karpushina
Keyword(s):  
Diagnostic Criteria ◽  
Peripheral Blood ◽  
Industrial Ecology ◽  
Laboratory Analysis ◽  
Immune Protection ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Modern Methods ◽  
The Impact

With the help of modern methods of laboratory analysis, peripheral blood monocytes and their production of TNF were evaluated as informative diagnostic criteria for the intensity of nonspecific immune protection in employees of sue «Moscow metro», experiencing the impact of adverse factors of industrial ecology.

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