scholarly journals Regulation of IL-12 p40 Promoter Activity in Primary Human Monocytes: Roles of NF-κB, CCAAT/Enhancer-Binding Protein β, and PU.1 and Identification of a Novel Repressor Element (GA-12) That Responds to IL-4 and Prostaglandin E2

2001 ◽  
Vol 167 (5) ◽  
pp. 2608-2618 ◽  
Author(s):  
Christoph Becker ◽  
Stefan Wirtz ◽  
Xiaojing Ma ◽  
Manfred Blessing ◽  
Peter R. Galle ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Promoter Activity ◽  
Binding Protein ◽  
Human Monocytes ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein ◽  
Repressor Element

Exchange protein directly activated by cAMP (EPAC) promotes transcriptional activation of the decidual prolactin gene via CCAAT/enhancer-binding protein in human endometrial stromal cells

2018 ◽  
Vol 30 (11) ◽  
pp. 1454
Author(s):  
Kazuya Kusama ◽  
Kazuhiro Tamura ◽  
Hanako Bai ◽  
Toshihiro Sakurai ◽  
Hirotaka Nishi ◽  
...  
Keyword(s):  
Promoter Activity ◽  
Binding Protein ◽  
Luciferase Assay ◽  
Mrna Levels ◽  
Reporter Activity ◽  
Camp Analogue ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein ◽  
Decidual Prolactin ◽  
Exchange Protein

Protein kinase A (PKA) signalling accompanies elevated intracellular cAMP levels during endometrial stromal cell (ESC) decidualisation. Exchange protein directly activated by cAMP (EPAC), an alternate mediator of cAMP signalling, promotes PKA analogue-induced decidualisation; however, the precise mechanism by which EPAC and PKA co-operatively stimulate decidualisation has not been characterised. To examine the role of CCAAT/enhancer-binding protein (C/EBP) in EPAC- and PKA-mediated decidualisation of primary human ESCs, a reporter plasmid containing the 332 bp region upstream from the transcription initiation site of the decidual prolactin (dPRL) gene was generated and the promoter activity was evaluated using a luciferase assay. The dPRL promoter activity was increased by treatment of transfected ESCs with the PKA-selective cAMP analogue N6-phenyl-cAMP (Phe) and enhanced further by co-treatment with the EPAC-selective cAMP analogue 8-(4-chlorophenyltio)-2′-O-methyl cAMP (CPT). Treatment with forskolin, an adenylyl cyclase activator, had a similar effect on reporter activity. Site-directed mutagenesis of the C/EBPβ- and/or C/EBPδ-binding site in the dPRL promoter abolished Phe/CPT-mediated elevation of the reporter activity. EPAC2 knockdown markedly reduced Phe-stimulated C/EBPβ and C/EBPδ mRNA levels, as well as forkhead box O1 (FOXO1) protein levels. These results suggest that EPAC signalling enhances PKA-mediated dPRL expression in ESCs by acting on C/EBP response elements in the promoter region of the gene.

scholarly journals Thapsigargin suppresses phorbol ester-dependent human involucrin promoter activity by suppressing CCAAT-enhancer-binding protein α (C/EBPα) DNA binding

2000 ◽  
Vol 350 (3) ◽  
pp. 791 ◽  
Author(s):  
Sivaprakasam BALASUBRAMANIAN ◽  
Chapla AGARWAL ◽  
Tatiana EFIMOVA ◽  
George R. DUBYAK ◽  
Eric BANKS ◽  
...  
Keyword(s):  
Dna Binding ◽  
Phorbol Ester ◽  
Promoter Activity ◽  
Binding Protein ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein ◽  
Involucrin Promoter
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