scholarly journals Overcoming T Cell Tolerance to the Hepatitis B Virus Surface Antigen in Hepatitis B Virus-Transgenic Mice

2001 ◽  
Vol 166 (2) ◽  
pp. 1389-1397 ◽  
Author(s):  
Alessandro D. Sette ◽  
Carla Oseroff ◽  
John Sidney ◽  
Jeff Alexander ◽  
Robert W. Chesnut ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
T Cell ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
T Cell Tolerance ◽  
Cell Tolerance ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus

Placebo-controlled trial of vaccination with hepatitis B virus surface antigen in hepatitis B virus transgenic mice

1997 ◽  
Vol 26 (1) ◽  
pp. 131-137 ◽  
Author(s):  
S.M. Fazle Akbar ◽  
Kazunori Kajino ◽  
Kenji Tanimoto ◽  
Kiyotaka Kurose ◽  
Toshikazu Masumoto ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Controlled Trial ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus

scholarly journals Partial Delipidation Improves the T-Cell Antigenicity of Hepatitis B Virus Surface Antigen

2006 ◽  
Vol 80 (7) ◽  
pp. 3506-3514 ◽  
Author(s):  
Isabelle Desombere ◽  
Annick Willems ◽  
Yvonne Gijbels ◽  
Geert Leroux-Roels
Keyword(s):  
Hepatitis B Virus ◽  
T Cell ◽  
Hepatitis B ◽  
B Cell ◽  
Surface Antigen ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Density Centrifugation ◽  
B Virus ◽  
Chronic Hbv

ABSTRACT Hepatitis B virus surface antigen (HBsAg) is a complex macromolecular particle composed of glycoproteins and lipids. The latter, representing 25% of the particle mass, are of host origin and determine the solubility, stability, and, indirectly, B-cell immunogenicity of HBsAg. HBsAg is a T-cell-dependent immunogen that does not elicit a detectable humoral immune response in 5% of HBsAg vaccine recipients and in most subjects suffering from chronic hepatitis B. We investigated the influence of the lipid content on the antigenicity of the particle. Lipids were partially removed from HBsAg by treatment with β-d-octyl glucoside and density centrifugation. Sham treatment consisted of density centrifugation of HBsAg only. We compared the in vitro proliferative responses of established T-cell lines and nonfractionated peripheral blood mononuclear cells (PBMC) from HBsAg vaccinees and chronic HBV patients when stimulated with partially delipidated HBsAg, untreated HBsAg, or sham-treated HBsAg. In all experiments, delipidated HBsAg turned out to be 10 to 100 times more antigenic than its untreated or sham-treated counterpart. Remarkably, PBMC from vaccine nonresponders or chronic HBV patients displayed a proliferative response towards delipidated HBsAg, whereas native HBsAg never induced a response. A series of control experiments demonstrated that this enhancement of T-cell antigenicity was HBsAg specific and directly linked to lipid extraction. Nonspecific adjuvant effects of any kind could be ruled out. In vivo evaluation in mice demonstrated that delipidated particles lose most of their B-cell antigenicity. However, when native and delipidated particles were mixed, these mixtures induced equal or slightly superior anti-HBs responses to those induced by the same quantity of native HBsAg alone. In conclusion, our data show that partial delipidation of HBsAg strikingly increases the T-cell antigenicity of this unique viral antigen.

Inhibition of hepatitis b virus surface antigen gene expression in carcinogen-induced liver tumors from transgenic mice

1994 ◽  
Vol 9 (4) ◽  
pp. 185-192 ◽  
Author(s):  
Hend Farza ◽  
Tommaso A. Dragani ◽  
Thomas Metzler ◽  
Giacomo Manenti ◽  
Pierre Tiollais ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Liver Tumors ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
Antigen Gene ◽  
B Virus

scholarly journals Demethylation by 5-Azacytidine Results in the Expression of Hepatitis B Virus Surface Antigen in Transgenic Mice

1989 ◽  
Vol 80 (4) ◽  
pp. 295-298 ◽  
Author(s):  
Kimi Araki ◽  
Jun-ichi Miyazaki ◽  
Toshiki Tsurimoto ◽  
Takeaki Inomoto ◽  
Tomohisa Iwanaga ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transgenic Mice ◽  
Surface Antigen ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus

Profiles of B and T cell immune responses elicited by different forms of the hepatitis B virus surface antigen

Vaccine ◽  
2010 ◽  
Vol 28 (45) ◽  
pp. 7288-7296 ◽  
Author(s):  
Meilong Shen ◽  
Shixia Wang ◽  
Guohong Ge ◽  
Yiping Xing ◽  
Xiuwen Ma ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
T Cell ◽  
Hepatitis B ◽  
Immune Responses ◽  
Surface Antigen ◽  
Virus Surface ◽  
Virus Surface Antigen ◽  
B Virus ◽  
T Cell Immune Responses
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