scholarly journals Phosphatidylinositol 4–Phosphate 5–Kinase α and Vav1 Mutual Cooperation in CD28-Mediated Actin Remodeling and Signaling Functions

2014 ◽  
Vol 194 (3) ◽  
pp. 1323-1333 ◽  
Author(s):  
Michela Muscolini ◽  
Cristina Camperio ◽  
Nicla Porciello ◽  
Silvana Caristi ◽  
Cristina Capuano ◽  
...  
Keyword(s):  
Mutual Cooperation ◽  
Actin Remodeling ◽  
Phosphatidylinositol 4

scholarly journals Inhibition of Phosphatidylinositol-4-phosphate 5-Kinase Iα Impairs Localized Actin Remodeling and Suppresses Phagocytosis

2002 ◽  
Vol 277 (46) ◽  
pp. 43849-43857 ◽  
Author(s):  
Marc G. Coppolino ◽  
Renee Dierckman ◽  
Joost Loijens ◽  
Richard F. Collins ◽  
Mahmoud Pouladi ◽  
...  
Keyword(s):  
Actin Remodeling ◽  
Phosphatidylinositol 4

scholarly journals Eukaryotic Elongation Factor 1A2 Cooperates with Phosphatidylinositol-4 Kinase III β To Stimulate Production of Filopodia through Increased Phosphatidylinositol-4,5 Bisphosphate Generation

2008 ◽  
Vol 28 (14) ◽  
pp. 4549-4561 ◽  
Author(s):  
Sujeeve Jeganathan ◽  
Anne Morrow ◽  
Anahita Amiri ◽  
Jonathan M. Lee
Keyword(s):  
Elongation Factor ◽  
Actin Remodeling ◽  
Cellular Processes ◽  
Eukaryotic Elongation Factor ◽  
Elongation Factor 1 Alpha ◽  
Membrane Bound ◽  
Elongation Factor 1A2 ◽  
Phosphatidylinositol 4 ◽  
Elongation Factor 1 ◽  
Transforming Gene

ABSTRACT Eukaryotic elongation factor 1 alpha 2 (eEF1A2) is a transforming gene product that is highly expressed in human tumors of the ovary, lung, and breast. eEF1A2 also stimulates actin remodeling, and the expression of this factor is sufficient to induce the formation of filopodia, long cellular processes composed of bundles of parallel actin filaments. Here, we find that eEF1A2 stimulates formation of filopodia by increasing the cellular abundance of cytosolic and plasma membrane-bound phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2]. We have previously reported that the eEF1A2 protein binds and activates phosphatidylinositol-4 kinase III beta (PI4KIIIβ), and we find that production of eEF1A2-dependent PI(4,5)P2 and generation of filopodia require PI4KIIIβ. Furthermore, PI4KIIIβ is itself capable of activating both the production of PI(4,5)P2 and the creation of filopodia. We propose a model for extrusion of filopodia in which eEF1A2 activates PI4KIIIβ, and activated PI4KIIIβ stimulates production of PI(4,5)P2 and filopodia by increasing PI4P abundance. Our work suggests an important role for both eEF1A2 and PI4KIIIβ in the control of PI(4,5)P2 signaling and actin remodeling.

scholarly journals Phosphatidylinositol 4-kinase IIβ negatively regulates invadopodia formation and suppresses an invasive cellular phenotype

2016 ◽  
Vol 27 (25) ◽  
pp. 4033-4042 ◽  
Author(s):  
Ganiyu Olabanji Alli-Balogun ◽  
Christina A. Gewinner ◽  
Ruth Jacobs ◽  
Janos Kriston-Vizi ◽  
Mark G. Waugh ◽  
...  
Keyword(s):  
Type I ◽  
Actin Remodeling ◽  
Clinically Significant ◽  
Membrane Type ◽  
Phosphatidylinositol 4 ◽  
Golgi Network ◽  
Interfering Rna ◽  
Cell Data ◽  
Invadopodia Formation ◽  
Mcf 7

The type II phosphatidylinositol 4-kinase (PI4KII) enzymes synthesize the lipid phosphatidylinositol 4-phosphate (PI(4)P), which has been detected at the Golgi complex and endosomal compartments and recruits clathrin adaptors. Despite common mechanistic similarities between the isoforms, the extent of their redundancy is unclear. We found that depletion of PI4KIIα and PI4KIIβ using small interfering RNA led to actin remodeling. Depletion of PI4KIIβ also induced the formation of invadopodia containing membrane type I matrix metalloproteinase (MT1-MMP). Depletion of PI4KII isoforms also differentially affected trans-Golgi network (TGN) pools of PI(4)P and post-TGN traffic. PI4KIIβ depletion caused increased MT1-MMP trafficking to invasive structures at the plasma membrane and was accompanied by reduced colocalization of MT1-MMP with membranes containing the endosomal markers Rab5 and Rab7 but increased localization with the exocytic Rab8. Depletion of PI4KIIβ was sufficient to confer an aggressive invasive phenotype on minimally invasive HeLa and MCF-7 cell lines. Mining oncogenomic databases revealed that loss of the PI4K2B allele and underexpression of PI4KIIβ mRNA are associated with human cancers. This finding supports the cell data and suggests that PI4KIIβ may be a clinically significant suppressor of invasion. We propose that PI4KIIβ synthesizes a pool of PI(4)P that maintains MT1-MMP traffic in the degradative pathway and suppresses the formation of invadopodia.

DESAIN PEMBELAJARAN BAHASA INDONESIA BERBASIS PEMAHAMAN NILAI SIKAP GOTONG ROYONG

2020 ◽  
Vol 13 (2) ◽  
pp. 01-10
Author(s):  
Alpansyah Alpansyah ◽  
Abdul Talib Hasim
Keyword(s):  
Experimental Study ◽  
Language Learning ◽  
Reader Response ◽  
Female Students ◽  
Male Students ◽  
Mutual Cooperation ◽  
Male And Female ◽  
Quasi Experimental ◽  
Bahasa Indonesia ◽  
Better Than

The aims of this study were: (1) to identify an increase in students' understanding of the value of mutual cooperation through the use of reader response rules in Indonesian Language Learning (KRPDPBI); (2) identifying the use of the reader response principle in Indonesian Language learning (KRPDPBI) there are differences between male and female students. The design of this study used a quasi-experimental study with two different methods. The results showed that (1) the achievement of the score of understanding the value of mutual cooperation for students taught by KRPDPBI was better than for students taught by regular learning according to the curriculum; (2) the achievement of the understanding of the value of male students' mutual cooperation is no better than that of female students.

IMPROVEMENT OF NATIONALISM REFLECTED IN PANCASILA'S 3rd SILA THROUGH THE ART OF REOG KENDHANG

2019 ◽  
Vol 1 (2) ◽  
pp. 69
Author(s):  
Andreas Andrie DJATMIKO
Keyword(s):  
Character Education ◽  
National Culture ◽  
Community Service ◽  
Positive Impact ◽  
Planned Change ◽  
Mutual Cooperation ◽  
Social Controls ◽  
Younger Generation ◽  
Negative Impacts ◽  
The Right

Community Service is one of the programs that are needed by the community today. This is done as an effort to respond to the needs of the community and is real program and realistic, especially in the fields of education, social, economic and cultural. Community Service Program is a program that is learning, studying and serving that is realized in the form of introduction and appreciation of community development through the clarity of planned change programs and problem solving methods regarding the ability to choose and use the right skills. A big nation is a nation that wants to respect its culture. Indonesia has a diversity of cultures that are characteristic and assets of the Indonesian nation. But most of the younger generation has forgotten a lot of the culture of the nation. The tendency of the younger generation now prefers outside cultures and many younger generations are less able to sort out most of which have negative impacts rather than a positive impact on themselves and others as well as the environment. There is a need for character education so that the younger generation can be relied upon in the future according to the nation's culture, such as being responsible for mutual cooperation, mutual assistance and good manners. Reog Kendhang's art proves that local culture can increase the sense of community nationalism and be able to become social controls so as not to fall into bad lifestyle. The formation of a national culture that can truly reunite all components of the nation's culture, therefore it is necessary to have a deeper introduction to history and cultural heritage in search of the identity of a pluralistic Indonesian society.

Rising to Grand Challenges: A Need for a Comprehensive Rusafrica Рroject

2018 ◽  
pp. 11-24
Author(s):  
Leonid Fituni
Keyword(s):  
Development Strategy ◽  
Technological Development ◽  
High Technology ◽  
Mutual Cooperation ◽  
Grand Challenges ◽  
Cooperative Approach ◽  
Technology Products ◽  
Innovative Products ◽  
The Russian Federation ◽  
Scientific And Technological Development

The author presents a vision of the mainstream vectors of global development against the backdrop of the “Grand Challenges” of the 21st century. He formulates optimal ways for Russia and Africa to interact in order to achieve the goals set by the UN Third International Conference on Financing for Development. The author proposes a RUSAFRICA project, which combines a dual goal of boosting economic, social and technological development of both Russia and Africa. The project envisages an integrated cooperative approach to mutually significant economic, technological and infrastructural requirements and capacities while prioritizing the human development aspect. Innovative approaches to mutual cooperation shale open ways to promoting Russian technologies and innovative products to new markets, generating growth of income from exports of high technology products and services with the aim to enhance Russia’s influence and competitive strengths, in accordance with the Scientific and Technological Development Strategy of the Russian Federation.

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