scholarly journals Platelet-Secreted MicroRNA-223 Promotes Endothelial Cell Apoptosis Induced by Advanced Glycation End Products via Targeting the Insulin-like Growth Factor 1 Receptor

2013 ◽  
Vol 192 (1) ◽  
pp. 437-446 ◽  
Author(s):  
Yi Pan ◽  
Hongwei Liang ◽  
Huan Liu ◽  
Donghai Li ◽  
Xi Chen ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Growth Factor ◽  
Advanced Glycation End Products ◽  
Cell Apoptosis ◽  
Insulin Like Growth Factor ◽  
Endothelial Cell Apoptosis ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products

1946-P: Insulin-Like Growth Factor-1 Protects against the Detrimental Effects of Advanced Glycation End Products and High Glucose in Myoblastic C2C12 Cells

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 1946-P
Author(s):  
IPPEI KANAZAWA ◽  
NAOKO ADACHI ◽  
KEN-ICHIRO TANAKA ◽  
AYUMU TAKENO ◽  
MASAKAZU NOTSU ◽  
...  
Keyword(s):  
Growth Factor ◽  
Advanced Glycation End Products ◽  
High Glucose ◽  
C2c12 Cells ◽  
Insulin Like Growth Factor ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products

scholarly journals Endogenous Secretory Receptor for Advanced Glycation End Products Protects Endothelial Cells from AGEs Induced Apoptosis

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Guomin Yang ◽  
Yinqiong Huang ◽  
Xiaohong Wu ◽  
Xiahong Lin ◽  
Jinting Xu ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Advanced Glycation End Products ◽  
Proinflammatory Cytokine ◽  
Enzyme Linked Immunosorbent Assay ◽  
Advanced Glycation ◽  
Expression Levels ◽  
Qrt Pcr ◽  
Glycation End Products ◽  
End Products

Endogenous secretory receptor for advanced glycation end products (esRAGE) binds extracellular RAGE ligands and blocks RAGE activation on the cell surface, protecting endothelial cell function. However, the underlying mechanism remains unclear. Endothelial cells overexpressing the esRAGE gene were generated using a lentiviral vector. Then, quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to assess esRAGE mRNA and protein levels, respectively. Hoechst-PI double staining was used to assess apoptosis. Western blot and qRT-PCR were used to assess the expression levels of apoptosis-related factors and the proinflammatory cytokine NF-кB. Compared with the control group, AGEs significantly induced endothelial cell apoptosis, which was significantly reduced by esRAGE overexpression. Incubation with AGEs upregulated the proapoptotic factor Bax and downregulated the antiapoptotic factor Bcl-2. Overexpression of esRAGE reduced Bax expression induced by AGEs and increased Bcl-2 levels. Furthermore, AGEs increased the expression levels of proinflammatory cytokine NF-кB, which were reduced after esRAGE overexpression. esRAGE protects endothelial cells from AGEs associated apoptosis, by downregulating proapoptotic (Bax) and inflammatory (NF-кB) factors and upregulating the antiapoptotic factor Bcl-2.

Sign in / Sign up

Export Citation Format

Share Document