scholarly journals Role of Caspases in Cytokine-Induced Barrier Breakdown in Human Brain Endothelial Cells

2012 ◽  
Vol 189 (6) ◽  
pp. 3130-3139 ◽  
Author(s):  
M. Alejandro Lopez-Ramirez ◽  
Roman Fischer ◽  
Claudia C. Torres-Badillo ◽  
Heather A. Davies ◽  
Karen Logan ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Brain ◽  
Brain Endothelial Cells ◽  
Barrier Breakdown

Autophagy inhibition preserves tight junction of human cerebral microvascular endothelium under oxygen glucose deprivation

2020 ◽  
Vol 17 ◽  
Author(s):  
Shuyan Wang ◽  
Jihong Xu ◽  
Jin Xi ◽  
John R. Grothusen ◽  
Renyu Liu
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Tight Junction ◽  
Human Brain ◽  
Cell Viability ◽  
Tight Junctions ◽  
Glucose Deprivation ◽  
Oxygen Glucose Deprivation ◽  
Brain Endothelial Cells

Aims:: To investigate the role of autophagy in the tight junction of human brain endothelial cells during hypoxia and ischemia. Background:: Endothelial cells play an important role in the initiation, progression and recovery from ischemic stroke. The role of autophagy on human brain endothelial cells (HBECs) subjected to oxygen-glucose deprivation (OGD) is not fully elucidated. Objective:: The objective of this study was to investigate the effect of autophagy on HBECs during OGD. Methods:: HBECs were cultured in a 96-well plate and underwent 4 hours of OGD. For drug treatment, 3-Methyladenine (3- MA) (5mmol/L), an inhibitor of autophagy, was added at the start of OGD. Cell viability and cytotoxicity were tested by cell counting kit-8 (CCK-8) and lactate dehydrogenase (LDH) assays. Morphological changes in cells were examined by immunofluorescence microscopy. The protein expression of light chain 3 (LC3) was measured. Autophagosomes and endothelial cell tight junctions were observed using transmission electron microscopy. Results:: The results showed that OGD induced serious damage to HBECs. Cell viability was decreased significantly and LDH release increased significantly (p<0.05) following OGD. 3-MA protected HBECs from damage. Immunostaining further confirmed these results. Since 3-MA is an inhibitor of autophagy, we chose to examine alterations in the amount of LC3, a marker of autophagy. The ratios of LC3-Ⅱ to LC3-Ⅰwere significantly lower in the 3-MA treated OGD group than in the non-3-MA treated OGD group (p<0.05). Electron microscopy showed that 3-MA inhibited the formation of autophagolysosomes and revealed that the tight junction ultrastructure of HBECs, which was destroyed by OGD, was significantly protected by treatment with 3-MA. Conclusions:: Autophagy is a key response to oxygen-glucose deprivation stress and its detrimental effects are closely related with destruction of tight junctions of human brain endothelial cells. Strategies to inhibit autophagy could help to preserve tight junctions.

scholarly journals Role of astrocytic leptin receptor subtypes on leptin permeation across hCMEC/D3 human brain endothelial cells

2010 ◽  
Vol 115 (5) ◽  
pp. 1288-1298 ◽  
Author(s):  
Hung Hsuchou ◽  
Abba J. Kastin ◽  
Hong Tu ◽  
N. Joan Abbott ◽  
Pierre-Olivier Couraud ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Brain ◽  
Leptin Receptor ◽  
Receptor Subtypes ◽  
Brain Endothelial Cells

Methamphetamine induces AP-1 and NF-?B binding and transactivation in human brain endothelial cells

2001 ◽  
Vol 66 (4) ◽  
pp. 583-591 ◽  
Author(s):  
Yong Woo Lee ◽  
Bernhard Hennig ◽  
Jin Yao ◽  
Michal Toborek
Keyword(s):  
Endothelial Cells ◽  
Human Brain ◽  
Brain Endothelial Cells

T lymphocytes conditioned with Interferon β induce membrane and soluble VCAM on human brain endothelial cells

2001 ◽  
Vol 115 (1-2) ◽  
pp. 161-167 ◽  
Author(s):  
Peter A. Calabresi ◽  
Alexandre Prat ◽  
Katherine Biernacki ◽  
Jessica Rollins ◽  
Jack P. Antel
Keyword(s):  
Endothelial Cells ◽  
T Lymphocytes ◽  
Human Brain ◽  
Brain Endothelial Cells ◽  
Interferon Β

NF-κB is activated and ICAM-1 gene expression is upregulated during reoxygenation of human brain endothelial cells

1998 ◽  
Vol 248 (3) ◽  
pp. 199-203 ◽  
Author(s):  
Eugene F Howard ◽  
Qiang Chen ◽  
Charles Cheng ◽  
James E Carroll ◽  
David Hess
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Human Brain ◽  
Brain Endothelial Cells
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