scholarly journals Pre-B Cell Colony-Enhancing Factor (PBEF/Nampt/Visfatin) Primes Neutrophils for Augmented Respiratory Burst Activity through Partial Assembly of the NADPH Oxidase

2011 ◽  
Vol 186 (11) ◽  
pp. 6474-6484 ◽  
Author(s):  
Zeenat Malam ◽  
Jean Parodo ◽  
Faiza Waheed ◽  
Katalin Szaszi ◽  
Andras Kapus ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
B Cell ◽  
Respiratory Burst ◽  
Burst Activity ◽  
Respiratory Burst Activity ◽  
Enhancing Factor ◽  
Cell Colony

scholarly journals Thyroid Hormone-Induced Cytosol-to-Nuclear Translocation of Rat Liver Nrf2 Is Dependent on Kupffer Cell Functioning

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Luis A. Videla ◽  
Pamela Cornejo ◽  
Pamela Romanque ◽  
Catherine Santibáñez ◽  
Iván Castillo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Nadph Oxidase ◽  
Kupffer Cell ◽  
Respiratory Burst ◽  
Oxidase Inhibitor ◽  
Burst Activity ◽  
Oxygen Species ◽  
Respiratory Burst Activity ◽  
Nadph Oxidase Inhibitor ◽  
Nrf2 Activation

L-3,3′,5-triiodothyronine (T3) administration upregulates nuclear factor-E2-related factor 2 (Nrf2) in rat liver, which is redox-sensitive transcription factor mediating cytoprotection. In this work, we studied the role of Kupffer cell respiratory burst activity, a process related to reactive oxygen species generation and liver homeostasis, in Nrf2 activation using the macrophage inactivator gadolinium chloride (GdCl3; 10 mg/kg i.v. 72 h before T3[0.1 mg/kg i.p.]) or NADPH oxidase inhibitor apocynin (1.5 mmol/L added to the drinking water for 7 days before T3), and determinations were performed 2 h after T3. T3increased nuclear/cytosolic Nrf2 content ratio and levels of heme oxygenase 1 (HO-1), catalytic subunit of glutamate cysteine ligase, and thioredoxin (Western blot) over control values, proteins whose gene transcription is induced by Nrf2. These changes were suppressed by GdCl3treatment prior to T3, an agent-eliciting Kupffer-cell depletion, inhibition of colloidal carbon phagocytosis, and the associated respiratory burst activity, with enhancement in nuclear inhibitor of Nrf2 kelch-like ECH-associated protein 1 (Keap1)/Nrf2 content ratios suggesting Nrf2 degradation. Under these conditions, T3-induced tumor necrosis factor-α(TNF-α) response was eliminated by previous GdCl3administration. Similar to GdCl3, apocynin given before T3significantly reduced liver Nrf2 activation and HO-1 expression, a NADPH oxidase inhibitor eliciting abolishment of colloidal carbon-induced respiratory burst activity without altering carbon phagocytosis. It is concluded that Kupffer cell functioning is essential for upregulation of liver Nrf2-signaling pathway by T3. This contention is supported by suppression of the respiratory burst activity of Kupffer cells and the associated reactive oxygen species production by GdCl3or apocynin given prior to T3, thus hindering Nrf2 activation.

scholarly journals Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor.

1994 ◽  
Vol 14 (2) ◽  
pp. 1431-1437 ◽  
Author(s):  
B Samal ◽  
Y Sun ◽  
G Stearns ◽  
C Xie ◽  
S Suggs ◽  
...  
Keyword(s):  
Stem Cell ◽  
B Cell ◽  
Stem Cell Factor ◽  
Human Peripheral Blood ◽  
Biological Activities ◽  
In Vitro Assays ◽  
Pokeweed Mitogen ◽  
Interleukin 7 ◽  
Enhancing Factor ◽  
Cell Colony

A novel gene coding for the pre-B-cell colony-enhancing factor (PBEF) has been isolated from a human peripheral blood lymphocyte cDNA library. The expression of this gene is induced by pokeweed mitogen and superinduced by cycloheximide. It is also induced in the T-lymphoblastoid cell line HUT 78 after phorbol ester (phorbol myristate acetate) treatment. The predominant mRNA for PBEF is approximately 2.4 kb long and codes for a 52-kDa secreted protein. The 3' untranslated region of the mRNA has multiple TATT motifs, usually found in cytokine and oncogene messages. The PBEF gene is mainly transcribed in human bone marrow, liver tissue, and muscle. We have expressed PBEF in COS 7 and PA317 cells and have tested the biological activities of the conditioned medium as well as the antibody-purified protein in different in vitro assays. PBEF itself had no activity but synergized the pre-B-cell colony formation activity of stem cell factor and interleukin 7. In the presence of PBEF, the number of pre-B-cell colonies was increased by at least 70% above the amount stimulated by stem cell factor plus interleukin 7. No effect of PBEF was found with cells of myeloid or erythroid lineages. These data define PBEF as a novel cytokine which acts on early B-lineage precursor cells.

scholarly journals Neutrophil phagocytosis and respiratory burst activity of dairy cows during the transition period and early lactation

2018 ◽  
Vol 12 (10) ◽  
pp. 894-903
Author(s):  
Marina Žekić-Stošić ◽  
Zdenko Kanački ◽  
Dragica Stojanović ◽  
Dejan Bugarski ◽  
Miodrag Lazarević ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Respiratory Burst ◽  
Transition Period ◽  
Hematological Parameters ◽  
Optimal Level ◽  
Burst Activity ◽  
Early Lactation ◽  
Health Production ◽  
Respiratory Burst Activity ◽  
Entire Study

Introduction: Hormonal and metabolic changes, as well as energy imbalance, can affect health, production and reproductive performance of dairy cows. In the present study, we evaluated phagocytosis and respiratory burst neutrophil activity during the transition period and early lactation and compared it with biochemical and hematological parameters in dairy cows. Methodology: Simmental cows (n = 21) were enrolled in the study. Whole blood samples were collected weekly from 3 weeks pre- calving until 6 weeks post calving. Basic metabolic and blood parameters were assessed by routine laboratory analyses, while neutrophil functions were analyzed by commercial test kits. Results: Optimal neutrophil response was observed pre and post calving. The highest value was recorded in the 6th week after calving (89.54 ± 7.61%) and being significantly higher (p < 0.01) as compared to values recorded at two and one week before and one week after calving. The percentage of activated neutrophils was high during the entire study period: from 70.80 ± 5.22% at the beginning of the study to 89.54 ± 7.61% at the end of the study. During the study period, production of Reactive Oxidative Species by neutrophils was positively correlated with β-hydroxybutyrat and non-esterified fatty acids values (0.454** and 0.423**, respectively) and calcium levels (0.164* and 0.212**, respectively). Conclusions: The most prominent changes in all parameters had no influence on phagocytic and respiratory burst activity of neutrophils. Neutrophil function is preserved at the optimal level during the transition period and early lactation in Simmental cows.

Ketamine inhibits polymorphonuclear leucocyte CD11b expression and respiratory burst activity in endotoxemic rats

2007 ◽  
Vol 56 (4) ◽  
pp. 149-153 ◽  
Author(s):  
Z. Q. Zhou ◽  
Y. Q. Yu ◽  
S. W. Feng ◽  
M. Yu ◽  
H. J. Liu ◽  
...  
Keyword(s):  
Respiratory Burst ◽  
Polymorphonuclear Leucocyte ◽  
Burst Activity ◽  
Respiratory Burst Activity ◽  
Cd11b Expression

scholarly journals Elevated Plasma Level of Visfatin/Pre-B Cell Colony-Enhancing Factor in Patients with Type 2 Diabetes Mellitus

2006 ◽  
Vol 91 (1) ◽  
pp. 295-299 ◽  
Author(s):  
Miao-Pei Chen ◽  
Fu-Mei Chung ◽  
Dao-Ming Chang ◽  
Jack C.-R. Tsai ◽  
Han-Fen Huang ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Plasma Level ◽  
B Cell ◽  
Elevated Plasma ◽  
Enhancing Factor ◽  
Cell Colony

Visfatin/PBEF/Nampt: structure, regulation and potential function of a novel adipokine

2008 ◽  
Vol 115 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Grit Sommer ◽  
Antje Garten ◽  
Stefanie Petzold ◽  
Annette G. Beck-Sickinger ◽  
Matthias Blüher ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
B Cell ◽  
Hormonal Regulation ◽  
Physiological Role ◽  
Nicotinamide Phosphoribosyltransferase ◽  
Insulin Mimetic ◽  
Enzymatic Function ◽  
Enhancing Factor ◽  
Structure Regulation ◽  
Cell Colony

Over the last few years, it has become obvious that obesity and insulin resistance are linked by a variety of proteins secreted by adipocytes. Visfatin/PBEF (pre-B-cell colony-enhancing factor) has recently been identified as a novel adipokine with insulin-mimetic effects. Furthermore, an enzymatic function has been reported that reveals visfatin/PBEF as Nampt (nicotinamide phosphoribosyltransferase; EC 2.4.2.12.). Moreover, reports on the structure and hormonal regulation of visfatin/PBEF/Nampt have given further insights into its potential physiological role. The present review summarizes studies on visfatin/PBEF/Nampt as a novel adipokine.

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