scholarly journals Induction of Mucin and MUC5AC Expression by the Protease Activity ofAspergillus fumigatusin Airway Epithelial Cells

2011 ◽  
Vol 187 (2) ◽  
pp. 999-1005 ◽  
Author(s):  
Tsuyoshi Oguma ◽  
Koichiro Asano ◽  
Katsuyoshi Tomomatsu ◽  
Motohiro Kodama ◽  
Koichi Fukunaga ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Protease Activity ◽  
Airway Epithelial Cells ◽  
Airway Epithelial

The Expression Profile of Protease Inhibitors in the Airway Epithelial Cells after Allergen (Der p 1) Stimulation

2021 ◽  
pp. 1-9
Author(s):  
Cagatay Karaaslan ◽  
Dilara Karaguzel ◽  
Basak Ezgi Sarac ◽  
Ceren Sucularli ◽  
Hayriye Akel Bilgic ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Protease Inhibitor ◽  
Protease Inhibitors ◽  
Protease Activity ◽  
Airway Epithelial Cells ◽  
Epithelial Barrier ◽  
Airway Epithelial ◽  
Time Points ◽  
Der P 1

<b><i>Background:</i></b> Airway epithelial cells are constantly exposed to intracellular and extracellular proteases that play a pivotal role in several airway diseases. <i>Dermatophagoides pteronyssinus</i> (Der p) 1 derived from house dust mite has protease activity that causes epithelial barrier defect and inflammatory response. Protease inhibitors released against proteases are involved in the maintenance of homeostasis. A disruption of the balance between proteases and protease inhibitors can lead to distortion of the cellular structures and cellular activities and thus culminate in disease processes. Although the effects of Der p 1 allergen on epithelial barrier integrity and inflammatory response are well-established, its contribution to protease inhibitor production is highly limited. <b><i>Objective:</i></b> This study aimed to determine the profile of the protease inhibitor response to Der p 1 allergen in human airway epithelial cells, A549 and BEAS-2B. <b><i>Methods:</i></b> Differentiated cells by the air-liquid interface were exposed to Der p 1 with or without Th2 type cytokines (IL-4 and IL-13). Gene expression of protease inhibitors was determined by qPCR at 2 different time points. <b><i>Results:</i></b> We found that the effect of allergen exposure on the protease inhibitor profile can vary depending on the antigen concentration, treatment duration, and the presence or absence of type 2 cytokines. Gene expressions of serine protease inhibitor (<i>SERPIN</i>)<i>B3</i> and <i>SERPINB4</i> were increased following Th2 cytokine stimulation in both cell types at both time points, whereas <i>SERPINB2</i> and <i>TFPI-2</i> expressions were induced by 24-h Der p 1 stimulation in both cells. <b><i>Conclusions:</i></b> Our study suggests that Der p 1 exposure of the airway epithelium may have consequences related to its protease activity in the presence as well as in the absence of Th2 cytokines in the microenvironment.

Differentiation of human pluripotent stem cells into airway epithelial cells

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
S Ulrich ◽  
S Weinreich ◽  
R Haller ◽  
S Menke ◽  
R Olmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelial Cells ◽  
Human Pluripotent Stem Cells ◽  
Airway Epithelial

scholarly journals ATP7B detoxifies silver in ciliated airway epithelial cells

2010 ◽  
Vol 243 (3) ◽  
pp. 315-322 ◽  
Author(s):  
Aida Ibricevic ◽  
Steven L. Brody ◽  
Wiley J. Youngs ◽  
Carolyn L. Cannon
Keyword(s):  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Airway Epithelial
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