scholarly journals Mannose-Binding Lectin (MBL) Substitution: Recovery of Opsonic Function In Vivo Lags behind MBL Serum Levels

2009 ◽  
Vol 183 (5) ◽  
pp. 3496-3504 ◽  
Author(s):  
Nannette Brouwer ◽  
Florine N. J. Frakking ◽  
Marianne D. van de Wetering ◽  
Michel van Houdt ◽  
Margreet Hart ◽  
...  
Keyword(s):  
Serum Levels ◽  
Mannose Binding Lectin ◽  
Mannose Binding ◽  
Binding Lectin

scholarly journals Mannose-Binding Lectin in Severe Acute Respiratory Syndrome Coronavirus Infection

2005 ◽  
Vol 191 (10) ◽  
pp. 1697-1704 ◽  
Author(s):  
W. K. Eddie Ip ◽  
Kwok Hung Chan ◽  
Helen K. W. Law ◽  
Gloria H. W. Tso ◽  
Eric K. P. Kong ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Serum Levels ◽  
Mannose Binding Lectin ◽  
In Vitro Assays ◽  
Calcium Dependent ◽  
Control Subjects ◽  
Mannose Binding ◽  
Carbohydrate Recognition Domains ◽  
Binding Lectin

Abstract Little is known about the innate immune response to severe acute respiratory syndrome (SARS) coronavirus (CoV) infection. Mannose-binding lectin (MBL), a key molecule in innate immunity, functions as an ante-antibody before the specific antibody response. Here, we describe a case-control study that included 569 patients with SARS and 1188 control subjects and used in vitro assays to investigate the role that MBL plays in SARS-CoV infection. The distribution of MBL gene polymorphisms was significantly different between patients with SARS and control subjects, with a higher frequency of haplotypes associated with low or deficient serum levels of MBL in patients with SARS than in control subjects. Serum levels of MBL were also significantly lower in patients with SARS than in control subjects. There was, however, no association between MBL genotypes, which are associated with low or deficient serum levels of MBL, and mortality related to SARS. MBL could bind SARS-CoV in a dose- and calcium-dependent and mannan-inhibitable fashion in vitro, suggesting that binding is through the carbohydrate recognition domains of MBL. Furthermore, deposition of complement C4 on SARS-CoV was enhanced by MBL. Inhibition of the infectivity of SARS-CoV by MBL in fetal rhesus kidney cells (FRhK-4) was also observed. These results suggest that MBL contributes to the first-line host defense against SARS-CoV and that MBL deficiency is a susceptibility factor for acquisition of SARS

Serum levels of mannose-binding lectin in systemic sclerosis: a possible contribution to the initiation of skin sclerosis in the diffuse cutaneous subtype

2014 ◽  
Vol 24 (1) ◽  
pp. 123-125 ◽  
Author(s):  
Kaname Akamata ◽  
Yoshihide Asano ◽  
Naohiko Aozasa ◽  
Shinji Noda ◽  
Takashi Taniguchi ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Serum Levels ◽  
Mannose Binding Lectin ◽  
Skin Sclerosis ◽  
Mannose Binding ◽  
Binding Lectin

Smoking status interacts with the association between mannose-binding lectin serum levels and gene polymorphism and the carriage of oropharyngeal bacteria

2010 ◽  
Vol 71 (3) ◽  
pp. 298-303 ◽  
Author(s):  
Ulla Jounio ◽  
Aino Rantala ◽  
Aini Bloigu ◽  
Raija Juvonen ◽  
Taina Lajunen ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Smoking Status ◽  
Serum Levels ◽  
Mannose Binding Lectin ◽  
Mannose Binding ◽  
Binding Lectin

scholarly journals Low Serum Levels of Mannose Binding Lectin Are a Risk Factor for Neonatal Sepsis

2007 ◽  
Vol 61 (3) ◽  
pp. 325-328 ◽  
Author(s):  
Fabrizio de Benedetti ◽  
Cinzia Auriti ◽  
Leila E D'Urbano ◽  
Maria Paola Ronchetti ◽  
Lucilla Ravà ◽  
...  
Keyword(s):  
Risk Factor ◽  
Neonatal Sepsis ◽  
Serum Levels ◽  
Mannose Binding Lectin ◽  
Mannose Binding ◽  
Binding Lectin ◽  
Low Serum

scholarly journals Mannose-binding lectin serum levels and (Gly54asp) gene polymorphism in recurrent aphthous stomatitis: A case-control study

2021 ◽  
Vol 35 ◽  
pp. 205873842110644
Author(s):  
Shereen A Baioumy ◽  
Shaimaa H Fouad ◽  
Shaimaa A Abdalgeleel ◽  
Ahmed A Baiomy ◽  
Dina E Sallam ◽  
...  
Keyword(s):  
Case Control Study ◽  
Serum Levels ◽  
Case Control ◽  
Recurrent Aphthous Stomatitis ◽  
Mannose Binding Lectin ◽  
Aphthous Stomatitis ◽  
Control Group ◽  
Mannose Binding ◽  
Binding Lectin ◽  
Control Study

Objectives: Dysregulation of the immune response appears to play a significant role in recurrent aphthous stomatitis (RAS) development. The main objective of this case–control study is to investigate the blood levels of mannose-binding lectin (MBL) and the frequency of the MBL2 gene (gly54asp) polymorphism in RAS patients, including 40 RAS patients and 40 healthy controls. Methods: Serum MBL levels were determined by ELISA, while the PCR-restriction fragment length polymorphism was used in MBL2 genotyping. Results: The median serum MBL level was significantly lower in the RAS group than in the control group (975 ng/mL (545–1320) vs. 1760 ng/mL (1254–2134); p≤ 0.001). The MBL levels were significantly lower in the BB genotype, whereas they were significantly higher in the wild type AA with a median of 525 and 1340 ng/mL, respectively ( p =0.005). The B allele was expressed in significantly higher percentages of RAS patients than in controls. There was no significant association between MBL serum levels ( p=0.685) or MBL2 codon 54 genotypes ( p=0.382) with the type of ulcers. Conclusion: There was an association between low MBL serum levels and the variant allele B of the MBL2 (gly54asp) gene, and the susceptibility to RAS. As a result, potential novel therapeutic options for RAS patients with MBL deficiency should be investigated.

scholarly journals Genetic Association With Pseudomonas aeruginosa Acquisition in Cystic Fibrosis: Influence of Surfactant Protein D and Mannose-Binding Lectin

2021 ◽  
Vol 12 ◽  
Author(s):  
Nasenien Nourkami-Tutdibi ◽  
Klemens Freitag ◽  
Michael Zemlin ◽  
Erol Tutdibi
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Genetic Variants ◽  
Serum Levels ◽  
Surfactant Protein ◽  
Mannose Binding Lectin ◽  
Surfactant Protein D ◽  
Risk Haplotype ◽  
Mannose Binding ◽  
Binding Lectin

Background:Pseudomonas aeruginosa (PA) infection in cystic fibrosis (CF) is associated with poor prognosis. Surfactant protein-D (SFTPD) and mannose-binding lectin (MBL) play a critical role in innate immunity and response to bacterial infections. We investigated serum levels and genetic variants of SFTPD and MBL in CF patients.Method: Thirty-five Caucasian patients homozygous for ΔF508del were genotyped for functional relevant polymorphisms within MBL2 (promoter−221 Y/X, codons 52, 54, and 57) and SFTPD genes (Met11Thr, Ala160Thr, and Ser270Thr). Serum levels of collectins, clinical characteristics, and PA status were correlated with genetic data.Results: Patients age, gender, and PA status did not affect MBL and SFTPD serum concentrations. MBL concentrations were correlated with MBL haplotypes. Patients with chronic Pseudomonas aeroginosa infection (PAC) and MBL insufficiency had a shorter interval between first PA infection and onset of PAC (0.01 vs. 4.6 years, p < 0.04) as well as a lower median age at transition to PAC (9.8 vs. 16.4 years, p < 0.03) compared to MBL sufficient patients with PAC. SFTPD serum level and FEV1% (Spearman r = −0.41, p < 0.03) showed a negative correlation irrespective of PA infection status. The hazard ratio to PA acquisition was increased in carriers of the SFTPD haplotype 11Thr-160Ala-270Ser compared to carriers of the common 11Met-160Thr-270Ser haplotype [HR 3.0 (95%CI: 1.1–8.6), p < 0.04].Conclusion: MBL insufficiency leads to a shorter interval between first PA infection and onset of chronic infection. Susceptibility to PA acquisition is associated with SFTPD genetic variants with 11Thr-160Ala-270Ser as risk haplotype for early PA infection. This may be due to presence of threonine associated with oligomeric structure of SFTPD and binding ability to bacteria.

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