scholarly journals Mouse Mast Cell Protease 4 Is the Major Chymase in Murine Airways and Has a Protective Role in Allergic Airway Inflammation

2009 ◽  
Vol 183 (10) ◽  
pp. 6369-6376 ◽  
Author(s):  
Ida Waern ◽  
Sofia Jonasson ◽  
Josephine Hjoberg ◽  
Anders Bucht ◽  
Magnus Åbrink ◽  
...  
Keyword(s):  
Mast Cell ◽  
Airway Inflammation ◽  
Allergic Airway Inflammation ◽  
Protective Role ◽  
Mast Cell Protease ◽  
Mouse Mast Cell

scholarly journals Mouse Mast Cell Protease-4-dependent production of ET-1 (1-31) and of plaque progression in an/INS; Apolipoprotein E knock-out mouse model of spontaneous atherosclerosis

Life Sciences ◽  
2013 ◽  
Vol 93 (25-26) ◽  
pp. e57
Author(s):  
Martin Houde ◽  
Walid Semaan ◽  
Louisane Desbiens ◽  
Zhipeng You ◽  
Adel G. Schwertani ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mouse Model ◽  
Apolipoprotein E ◽  
Plaque Progression ◽  
Knock Out ◽  
Mast Cell Protease ◽  
Mouse Mast Cell ◽  
Knock Out Mouse

Human mouse mast cell protease 7–like tryptase genes are pseudogenes

2001 ◽  
Vol 107 (2) ◽  
pp. 315-321 ◽  
Author(s):  
Hae-Ki Min ◽  
Naotomo Kambe ◽  
Lawrence B. Schwartz
Keyword(s):  
Mast Cell ◽  
Mast Cell Protease ◽  
Mouse Mast Cell

scholarly journals Biochemical and immunological characterization of multiple glycoforms of mouse mast cell protease 1: comparison with an isolated murine serosal mast cell protease (MMCP-4)

1993 ◽  
Vol 294 (1) ◽  
pp. 127-135 ◽  
Author(s):  
G F J Newlands ◽  
D P Knox ◽  
S R Pirie-Shepherd ◽  
H R P Miller
Keyword(s):  
Mast Cell ◽  
Amino Acid ◽  
Mast Cells ◽  
Western Blotting ◽  
Trichinella Spiralis ◽  
Double Diffusion ◽  
Terminal Amino Acid ◽  
Mast Cell Protease ◽  
Terminal Amino ◽  
Mouse Mast Cell

Five highly soluble, chymotrypsin-like, neutral serine proteases, with molecular masses in the range 30-33 kDa, were isolated from Trichinella spiralis-infected mouse small intestine. These enzymes were closely related antigenically on Western blotting and by Ouchterlony double diffusion using a polyclonal, cross-absorbed, sheep antibody raised against mouse mast cell protease-1 (MMCP-1) and on the basis of N-terminal amino acid sequence analysis, were identified as variant forms of MMCP-1. Substrate and inhibitor analysis confirmed that the five variants (MMCP-1 A-E) had similar characteristics, although highly significant (P = 0.025 to P < 0.0001) variations in Km and kcat, were detected. Against human alpha 1-proteinase inhibitor the Ki for MMCP-1C (45 pM) was significantly (P < 0.0001) greater than those for the other proteases (0.76-2.2 pM). The differences in electrophoretic mobility are probably a result of variable glycosylation, since removal of N-linked carbohydrate produced a polypeptide of approx. 28 kDa in each case which was, like the native enzyme, immunoreactive on Western blotting. A much less soluble 28 kDa enzyme was isolated from serosal mast cells and identified as MMCP-4 by N-terminal amino acid sequencing. Like MMCP-1 it has chymotrypsin-like substrate specificities with activity at neutral pH. However, it was antigenically distinct from MMCP-1 and, using sheep anti-MMCP-1, was not detected on Western blotting or by Ouchterlony double diffusion, e.l.i.s.a. or immunohistochemistry. This last technique established that the MMCP-1 variants were uniquely present in enteric mast cells, thereby providing a highly selective means of distinguishing the mucosal and connective tissue mast cell subsets in the mouse.

scholarly journals HDAC2 attenuates airway inflammation by suppressing IL-17A production in HDM-challenged mice

2019 ◽  
Vol 316 (1) ◽  
pp. L269-L279 ◽  
Author(s):  
Tianwen Lai ◽  
Mindan Wu ◽  
Chao Zhang ◽  
Luanqing Che ◽  
Feng Xu ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Inflammatory Cytokines ◽  
Allergic Inflammation ◽  
Allergic Airway Inflammation ◽  
Inflammatory Cells ◽  
Protective Role ◽  
In Vivo Models

Histone deacetylase (HDAC)2 is expressed in airway epithelium and plays a pivotal role in inflammatory cells. However, the role of HDAC2 in allergic airway inflammation remains poorly understood. In the present study, we determined the role of HDAC2 in airway inflammation using in vivo models of house dust mite (HDM)-induced allergic inflammation and in vitro cultures of human bronchial epithelial (HBE) cells exposed to HDM, IL-17A, or both. We observed that HDM-challenged Hdac2+/− mice exhibited substantially enhanced infiltration of inflammatory cells. Higher levels of T helper 2 cytokines and IL-17A expression were found in lung tissues of HDM-challenged Hdac2+/− mice. Interestingly, IL-17A deletion or anti-IL-17A treatment reversed the enhanced airway inflammation induced by HDAC2 impairment. In vitro, HDM and IL-17A synergistically decreased HDAC2 expression in HBE cells. HDAC2 gene silencing further enhanced HDM- and/or IL-17A-induced inflammatory cytokines in HBE cells. HDAC2 overexpresion or blocking IL-17A gene expression restored the enhanced inflammatory cytokines. Collectively, these results support a protective role of HDAC2 in HDM-induced airway inflammation by suppressing IL-17A production and might suggest that activation of HDAC2 and/or inhibition of IL-17A production could prevent the development of allergic airway inflammation.

Constitutive secretion of the granule chymase mouse mast cell protease-1 and the chemokine, CCL2, by mucosal mast cell homologues

2003 ◽  
Vol 33 (1) ◽  
pp. 132-146 ◽  
Author(s):  
J. K. Brown ◽  
P. A. Knight ◽  
S. H. Wright ◽  
E. M. Thornton ◽  
H. R. P. Miller
Keyword(s):  
Mast Cell ◽  
Mucosal Mast Cell ◽  
Mast Cell Protease ◽  
Constitutive Secretion ◽  
Mouse Mast Cell

scholarly journals Mucosal Defense against Gastrointestinal Nematodes: Responses of Mucosal Mast Cells and Mouse Mast Cell Protease 1 during PrimaryStrongyloides venezuelensis Infection in FcRγ-Knockout Mice

2000 ◽  
Vol 68 (9) ◽  
pp. 4968-4971 ◽  
Author(s):  
Denis N. Onah ◽  
Fukumi Uchiyama ◽  
Yuuko Nagakui ◽  
Masao Ono ◽  
Toshiyuki Takai ◽  
...  
Keyword(s):  
Mast Cell ◽  
Gastrointestinal Nematodes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mucosal Mast Cell ◽  
Mast Cell Protease ◽  
Intestinal Nematode ◽  
Nematode Parasites ◽  
Cell Counts ◽  
Strongyloides Venezuelensis ◽  
Mouse Mast Cell

ABSTRACT A possible role for the γ subunit of immunoglobulin Fc receptors (FcR) in mucosal defenses against intestinal nematode parasites was studied using age-matched FcRγ-knockout (FcRγ−/−) and wild-type (FcRγ+/+) C57BL/6 mice. Mice were infected subcutaneously with 3,000 infective larvae of Strongyloides venezuelensis, and the degree of infection was monitored by daily fecal egg counts and adult worm recovery on days 8 and 13 postinfection. Mucosal mast cell (MMC) responses were assayed by in situ intestinal mast cell counts in stained histological sections of the jejunum and by measuring mouse mast cell protease 1 (MMCP-1) release in serum using sandwich enzyme-linked immunosorbent assay. FcRγ−/− mice had significantly higher egg counts (P < 0.01) and numbers of adult worms (P < 0.05) than FcRγ+/+mice, but mastocytosis and serum MMCP-1 release were comparable. It was concluded that MMCP-1 release may be spontaneous, does not depend on mast cell degranulation via the FcRγ signaling system, and appears to play no role in the expulsion of S. venezuelensis. The delay in worm expulsion in the FcRγ−/− mice might be related to inability of the MMC to degranulate and release effector molecules other than MMCP-1, since FcRγ deletion abrogates mast cell degranulative responses.

scholarly journals Mouse Mast Cell Protease 4 Deletion Protects Heart Function and Survival After Permanent Myocardial Infarction

2018 ◽  
Vol 9 ◽  
Author(s):  
Martin Houde ◽  
Adel Schwertani ◽  
Hanène Touil ◽  
Louisane Desbiens ◽  
Otman Sarrhini ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Mast Cell ◽  
Heart Function ◽  
Mast Cell Protease ◽  
Mouse Mast Cell

scholarly journals Deficiency of mouse mast cell protease 4 mitigates cardiac dysfunctions in mice after myocardium infarction

2019 ◽  
Vol 1865 (6) ◽  
pp. 1170-1181 ◽  
Author(s):  
Yunzhe Wang ◽  
Cong-Lin Liu ◽  
Wenqian Fang ◽  
Xian Zhang ◽  
Chongzhe Yang ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cell Protease ◽  
Myocardium Infarction ◽  
Mouse Mast Cell
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