scholarly journals Human Embryonic Stem Cells Suppress T Cell Responses via Arginase I-Dependent Mechanism

2009 ◽  
Vol 184 (3) ◽  
pp. 1300-1308 ◽  
Author(s):  
Nurit Yachimovich-Cohen ◽  
Sharona Even-Ram ◽  
Yoel Shufaro ◽  
Jacob Rachmilewitz ◽  
Benjamin Reubinoff
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
T Cell Responses ◽  
Cell Responses ◽  
Arginase I ◽  
Dependent Mechanism

Stimulation of Potent Antigen-Specific T-Cell Responses Using Semiallogenic Dendritic Cells Derived from Human Embryonic Stem Cells.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3704-3704
Author(s):  
Zhen Su ◽  
Fusheng Wei ◽  
Susan Fesperman ◽  
Siqing Wang ◽  
Philipp Dahm ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dendritic Cells ◽  
T Cell ◽  
Embryonic Stem Cells ◽  
Cell Line ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Hematopoietic Stem ◽  
Gm Csf ◽  
Cell Responses

Abstract The major objective of this study is to develop a novel and broadly applicable immunotherapy platform against cancer and infectious diseases. We hypothesized that human embryonic stem cells (hESC) could serve as a source for generating dendritic cells (DC) with potent immunostimulatory function. One advantage of using hESC-derived DC in clinical settings is the ability to generate virtually unlimited amounts of antigen presenting cells for vaccination. Although hESC-derived DC are not genetically identical to the recipient patient, antigen processing and presentation can be facilitated by matching hESC to recipients that share HLA class I alleles. Another advantage of this technology is that hESC express highly polymorphic HLA class II molecules that serve as major rejection antigens, thereby augmenting the antigen-specific T-cell response in the cancer patient. In the current study, we have established a novel three-step method to differentiate hESC (H9 cell line) into mature DC sequentially through hematopoietic stem cell and myeloid precursor stages. During the first step, 10–15% CD34+ hematopoietic stem cells were generated by co-culturing hESCs with the bone marrow stromal cell line OP9. During the second step, these CD34+ hematopoietic stem cells were differentiated into CD45+CD33+ myeloid precursors and further expanded in the presence of GM-CSF. In the final step, all myeloid precursors were differentiated into mature DC using a cytokine cocktail including GM-CSF, Flt-3L and TNF-α. Using this method, we were able to generate approximately 1 × 108 mature hESC-derived DCs (hESDC) with ≥ 80% purity. These hESDC exhibited a similar phenotype than monocyte-derived DC with high expression of MHC class I, MHC class II, CD11c, CD54, CD40 and the co-stimulatory molecule CD86. Upon activation with proinflammatory cytokines, the hESDC secreted IL-12p70 and migrated in response to MIP-3β. In mixed lymphocyte reaction assays, hESDC exhibited strong allo-stimulatory capacity. Moreover, peptide-loaded mature hESDCs were able to stimulate antigen-specific CD8+ T-cell responses against the EBV peptide BMLF1280-288 and the MART-1 peptide (ELAGIGILTV) in a HLA-A2-restricted manner. Most importantly, hESDC stimulated HLA-A2+ MART-1 peptide-specific CD8+ T cells in vitro that were capable of recognizing and killing the HLA-A2+ melanoma cell line Malena-3M. These data suggest the development of a scalable DC platform that could be applied in clinical immunotherapy protocols.

scholarly journals Human Embryonic Stem Cells Prevent T-Cell Activation by Suppressing Dendritic Cells Function via TGF-Beta Signaling Pathway

Stem Cells ◽  
2014 ◽  
Vol 32 (12) ◽  
pp. 3137-3149 ◽  
Author(s):  
Lucy Leshansky ◽  
Daniel Aberdam ◽  
Joseph Itskovitz-Eldor ◽  
Sonia Berrih-Aknin
Keyword(s):  
Stem Cells ◽  
Dendritic Cells ◽  
T Cell ◽  
Embryonic Stem Cells ◽  
Signaling Pathway ◽  
T Cell Activation ◽  
Human Embryonic Stem Cells ◽  
Cell Activation ◽  
Embryonic Stem ◽  
Tgf Beta

scholarly journals Generation of Functional Thymic Epithelium from Human Embryonic Stem Cells that Supports Host T Cell Development

2013 ◽  
Vol 13 (2) ◽  
pp. 219-229 ◽  
Author(s):  
Audrey V. Parent ◽  
Holger A. Russ ◽  
Imran S. Khan ◽  
Taylor N. LaFlam ◽  
Todd C. Metzger ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
T Cell Development ◽  
Embryonic Stem ◽  
Cell Development ◽  
Thymic Epithelium

Thymopentin enhances the generation of T-cell lineage derived from human embryonic stem cells in vitro

2015 ◽  
Vol 331 (2) ◽  
pp. 387-398 ◽  
Author(s):  
Ming-Xia Zhu ◽  
Wen-Li Wan ◽  
Hai-Shen Li ◽  
Jing Wang ◽  
Gui-An Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cell ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Lineage

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression
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