scholarly journals CD44highMemory CD8 T Cells Synergize with CpG DNA to Activate Dendritic Cell IL-12p70 Production

2009 ◽  
Vol 183 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Kok Loon Wong ◽  
Li Fang Melissa Tang ◽  
Fei Chuin Lew ◽  
Hok Sum Kenneth Wong ◽  
Yen Leong Chua ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cell ◽  
Cd8 T Cells ◽  
Cpg Dna

scholarly journals Superior Protective Immunity against Murine Listeriosis by Combined Vaccination with CpG DNA and Recombinant Salmonella enterica Serovar Typhimurium

2009 ◽  
Vol 77 (12) ◽  
pp. 5501-5508 ◽  
Author(s):  
Christina Berchtold ◽  
Klaus Panthel ◽  
Stefan Jellbauer ◽  
Brigitte Köhn ◽  
Elisabeth Roider ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cd8 T Cells ◽  
Salmonella Enterica ◽  
Protective Immunity ◽  
Cd8 T Cell ◽  
Cpg Dna ◽  
Memory Cd8 T Cells ◽  
Type Iii ◽  
Serovar Typhimurium

ABSTRACT Preexisting antivector immunity can severely compromise the ability of Salmonella enterica serovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. To circumvent this problem, we injected CpG DNA admixed to the immunodominant p60217-225 peptide from Listeria monocytogenes subcutaneously into BALB/c mice and coadministered a p60-translocating Salmonella strain by the orogastric route. The distribution of tetramer-positive p60217-225-specific effector and memory CD8 T cells was analyzed by costaining of lymphocytes with CD62L and CD127. In contrast to the single oral application of recombinant Salmonella or single immunization with CpG and p60, in the spleens from mice immunized with a combination of both vaccine types a significantly higher level of p60-specific CD8 T cells with a predominance of the effector memory T-cell subset was detected. In vivo protection studies revealed that this CD8 T-cell population conferred sterile protective immunity against a lethal infection with L. monocytogenes. However, p60-specific central memory CD8 T cells induced by single vaccination with CpG and p60 were not able confer effective protection against rapidly replicating intracellular Listeria. In conclusion, we provide compelling evidence that the combination of Salmonella type III-mediated antigen delivery and CpG immunization is an attractive novel vaccination strategy to modulate CD8 differentiation patterns toward distinct antigen-specific T-cell subsets with favorable protective capacities.

scholarly journals CD8 T cells targeting adapted epitopes in chronic HIV infection promote dendritic cell maturation and CD4 T cell trans-infection

2019 ◽  
Vol 15 (8) ◽  
pp. e1007970 ◽  
Author(s):  
Kai Qin ◽  
Sushma Boppana ◽  
Victor Y. Du ◽  
Jonathan M. Carlson ◽  
Ling Yue ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Dendritic Cell ◽  
Hiv Infection ◽  
Cd8 T Cells ◽  
Dendritic Cell Maturation ◽  
Cd4 T Cell ◽  
Cell Maturation ◽  
Trans Infection ◽  
Chronic Hiv Infection

Cross-Linking FcεRIα on a Dendritic Cell Line Induces Increased IFNγ Production from CD8+ T Cells

2006 ◽  
Vol 117 (2) ◽  
pp. S243
Author(s):  
D.S. Cheung ◽  
K.M. Shaw ◽  
M.M. Rohlfing ◽  
M.H. Grayson
Keyword(s):  
T Cells ◽  
Dendritic Cell ◽  
Cell Line ◽  
Cd8 T Cells ◽  
Cross Linking

The Novel Bispecific Diabody αCD40/αCD28 Strengthens Leukemic Dendritic Cell Induced T Cell Reactivity.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3710-3710
Author(s):  
Ilse Houtenbos ◽  
Saskia J.A.M. Santegoets ◽  
Theresia M. Westers ◽  
Quinten Waisfisz ◽  
Sergey Kipriyanov ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cells ◽  
T Cell ◽  
Dendritic Cell ◽  
Cd8 T Cells ◽  
Cluster Formation ◽  
T Cell Proliferation ◽  
Cross Linking ◽  
Bispecific Diabody ◽  
Control Protein

Abstract Dendritic cell (DC)-based immunotherapy faces new challenges since efficacy of DC vaccines in clinical trials has been inconsistent. Strategies to improve immune responses induced by DC are currently being explored. We have recently shown the feasibility of generating fully functional DC from Acute Myeloid Leukemic (AML) blasts, but with varying expression levels of the important costimulatory molecule CD86. To overcome this variability, we developed a novel bispecific diabody (BsDb) simultaneously and agonistically targeting CD40 on AML-DC and CD28 on naïve T cells. Beside optimization of CD28-mediated signaling, the resulting cellular cross-linking was also hypothesized to increase the strength and duration of T cell/AML-DC interactions, thus increasing T cell responsiveness to AML antigens. Indeed the αCD40/αCD28-bispecific diabody provokes increased T cell-DC cluster formation as assessed by light microscopy. Significant increased cluster formation was observed when T cells and AML-DC were cocultured in presence of the BsDb as compared to T cells incubated with a control protein (46%±2 versus 22%±1 respectively, p<0.05). Prior incubation of T cells and/or AML-DC with CD28 or CD40, respectively, completely prevented cluster formation in presence of the BsDb indicating specific binding of the BsDb to CD40 and CD28. The αCD40/αCD28 BsDb significantly increases T cell proliferation induced by AML-DC as compared to the unstimulated cocultures, in a dose dependent manner, as evaluated by mixed lymphocyte reactions (fold increased T cell proliferation of cocultures stimulated with BsDb as compared to unstimulated cocultures:170%±12, p<0.05). In addition, BsDb is capable of DC maturation induction as shown by significant increased mean fluorescence index (MFI) of the maturation markers CD80 (MFI of AML-DC cultured in presence of control protein vs AML-DC cultured in presence of BsDb: 22±5 vs 12±3, p<0.05) and CD83 (4±1 vs 1.5±0.5, p<0.05). In order to determine the effect of aCD40/aCD28-bispecific diabody-mediated cross-linking of AML-derived DC and CD8+ T cells on the induction efficiency of tumor-specific CTL, AML-DC derived from the HLA-A2+ AML cell line MUTZ-3 were pre-incubated with the aCD40/aCD28-bispecific diabody, loaded with the heteroclitic variant of the aa988 epitope of hTERT, and used as stimulator cells in an HLA-A2-matched allogeneic in vitro CTL induction protocol. In total nine parallel bulk cultures, were stimulated twice with peptide-loaded MUTZ-3 DC, either pulsed with control protein or the aCD40/aCD28-bispecific diabody. hTERT988Y-specific CD8+ T cells could be detected in 5/9 individual cultures when stimulated with DC pulsed with the aCD40/aCD28-bispecific diabody, whereas in only 1/9 individual cultures hTERT988Y-specific CD8+ T cells could be detected when stimulated with DC pulsed with the control protein. Thus, priming efficacy of tumor-specific cytotoxic T cells can also be improved by cross-linking AML-DC and T cells with the αCD40/αCD28 diabody. We propose that the αCD40/αCD28-bispecific diabody can serve as a potent therapeutic tool to effectively augment anti-tumor T cell responses elicited by AML-DC.

scholarly journals Early cytoskeletal rearrangement during dendritic cell maturation enhances synapse formation and Ca2+ signaling in CD8+ T cells

2004 ◽  
Vol 34 (10) ◽  
pp. 2708-2719 ◽  
Author(s):  
Marco Averbeck ◽  
Thorsten Braun ◽  
Gunther Pfeifer ◽  
Jonathan Sleeman ◽  
Jan Dudda ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cell ◽  
Cd8 T Cells ◽  
Synapse Formation ◽  
Dendritic Cell Maturation ◽  
Cell Maturation ◽  
Cytoskeletal Rearrangement

scholarly journals Virulence ofToxoplasma gondiiIs Associated with Distinct Dendritic Cell Responses and Reduced Numbers of Activated CD8+T Cells

2010 ◽  
Vol 185 (3) ◽  
pp. 1502-1512 ◽  
Author(s):  
Elia D. Tait ◽  
Kimberly A. Jordan ◽  
Christopher D. Dupont ◽  
Tajie H. Harris ◽  
Beth Gregg ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cell ◽  
Cd8 T Cells ◽  
Cell Responses
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