scholarly journals Comparative performance of Line Probe Assay (version 2) and Xpert® MTB/RIF assay for early diagnosis of rifampicin resistant pulmonary tuberculosis

2021 ◽  
Author(s):  
Raj Narayan Yadav ◽  
Binit Kumar Singh ◽  
Rohini Sharma ◽  
Jigyasa Chaubey ◽  
Sanjeev Sinha ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Pulmonary Tuberculosis ◽  
Line Probe Assay ◽  
Comparative Performance ◽  
Probe Assay

scholarly journals A study on mutation in genes associated with rifampicin and isoniazid resistance in Mycobacterium tuberculosis complex using line probe assay

2018 ◽  
Vol 6 (12) ◽  
pp. 3997
Author(s):  
Rohit Kumar ◽  
Shivendra Kumar Shahi ◽  
Rakesh Kumar ◽  
Balkrishna Mishra ◽  
Shailesh Kumar ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Early Diagnosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Smear Microscopy ◽  
Common Mutation ◽  
Line Probe Assay ◽  
Tuberculosis Complex ◽  
Sensitivity Pattern ◽  
Probe Assay

Background: The term tuberculosis describe a clinical illness, which is predominantly caused by Mycobacterium tuberculosis and less common by other species. Infection is transmitted by infected droplets through respiratory route. Early diagnosis and appropriate management is the only way to control the spread of infection. The available diagnostic tools include, smear microscopy, culture and molecular methods. Culture is the gold standard, but it takes around 2-8 weeks to get the result and smear microscopy having less sensitivity. Molecular technique especially Line probe assay can be better option because of high sensitivity and specificity, and directly clinical sample can be used, and result will be made available within same day with sensitivity pattern. Present study was designed to use of LPA for early diagnosis.Methods: Laboratory based observational study conducted in department of microbiology, IGIMS Patna and TBDC, Patna. Sputum specimens were collected from clinically suspected cases of pulmonary tuberculosis, and subjected to smear microscopy, culture and LPA.Results: During the study period, 2841 patients were diagnosed as pulmonary tuberculosis. Strain of Mycobacterium tuberculosis complex in, 12% (347) patients were rifampicin and isoniazid resistant, 4% (117) and 3% (86) patients were rifampicin and isoniazid mono-resistant respectively. We found that rpoB MUT3 was the most common mutation in gene associated with rifampicin resistant and katG MUT1gene associated with isoniazid resistant.Conclusions: Present study support the use of LPA for early diagnosis of smear positive as well as smear negative pulmonary tuberculosis cases. Resulting early diagnosis and appropriate management of patients.

scholarly journals Xpert MTB/Rif assay in the diagnosis of smear-negative pulmonary tuberculosis: a prospective study from Nepal

2019 ◽  
Author(s):  
Priyatam Khadka ◽  
Januka Thapaliya ◽  
Ramesh Bahadur Basnet ◽  
Gokarna Raj Ghimire ◽  
Jyoti Amatya ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Disease Transmission ◽  
Clinical Information ◽  
University Teaching ◽  
Smear Microscopy ◽  
Line Probe Assay ◽  
Smear Negative ◽  
Negative Sputum ◽  
Probe Assay ◽  
A Prospective Study

Abstract Background For improving patient care and abbreviating the disease transmission chain, speedy detection of tuberculosis and its drug-resistance with precision is crucial. Methods We analyzed, pulmonary tuberculosis (PTB) suspected, 360 smear-negative sputum from the patients attending Tribhuvan University Teaching Hospital (TUTH). The patients were selected as per the algorithm of National Tuberculosis Programme(NTP) for Xpert MTB/RIF testing. Participants’ demographic and clinical information were collected using a pre-tested questionnaire. The specimens were collected, processed directly for Xpert MTB/RIF test according to the manufacturer’s protocol. The same samples were stained using Ziehl-Neelsen technique then observed microscopically. Both findings were interpreted; rifampicin-resistant, if obtained, on Xpert testing was confirmed with Line Probe Assay. Result Of 360 smear-negative sputum samples analyzed, 85(23.61%) found positive while 3 of them were rifampicin resistance. The infection was higher in male, i.e. 60(25.3%) compared to female 25(20.3%). The age group, >45(nearly 33%) with median age 42± 21.5, were prone to the infection. During the study period, 4.6% (515/11048) sputum samples were reported as smear-positive in TUTH; consequently, with Xpert MTB/RIF assay, additional case 16.5% (n=85/515) missed on the smear microscopy, were detected—surging overall confirmed cases. Among the most occurring clinical presentations, cough and chest pain were more evident in PTB with relative-risk at 95% confident-levels i.e. 3.03(1.01-9.11) and 3.47(2.29-5.27) respectively. A higher number of new suspects (n=63) were found positive compared to previously treated suspects. The upper lobe infiltrates (36.4%) and pleural effusion (40.4%) were peculiar radiological impression noted in PTB patient. 94 MDR suspected cases were enrolled; of total suspected cases, 29 samples were found rifampicin sensitive, 1 indeterminant while 2 of them were rifampicin-resistant. However, a single rifampicin-resistant; case was detected in patient which was not MDR suspected. Conclusion Additional cases of PTB which are neglected as smear-negative on microscopy and other conventional tests can be detected with gene Xpert test. Hence, recommended to every suspect as a presumptive test could be a wise investment in diagnosis to restrict the global burden to some extent. Keywords: Xpert MTB/Rif assay, Mycobacterium tuberculosis, Line Probe Assay, MDR-TB

scholarly journals Diagnostic utility of a line probe assay for multidrug resistant-TB in smear-negative pulmonary tuberculosis

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0182988 ◽  
Author(s):  
Binit Kumar Singh ◽  
Surendra K. Sharma ◽  
Rohini Sharma ◽  
Vishnubhatla Sreenivas ◽  
Vithal P. Myneedu ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Multidrug Resistant ◽  
Diagnostic Utility ◽  
Line Probe Assay ◽  
Smear Negative ◽  
Probe Assay

Evaluation of use of line probe assay on smear-positive direct specimen from extra-pulmonary tuberculosis site

2015 ◽  
Vol 62 (4) ◽  
pp. 218-221 ◽  
Author(s):  
Paresh Dave ◽  
Pranav Patel ◽  
Bhavesh Modi ◽  
Prakash Patel ◽  
Bhavin Vadera ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Line Probe Assay ◽  
Extra Pulmonary Tuberculosis ◽  
Extra Pulmonary ◽  
Probe Assay ◽  
Positive Direct

scholarly journals Improving the diagnosis of pulmonary tuberculosis using line probe assay and determining the factors associated with the disease in children in Jos, Nigeria

GERMS ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 328-337
Author(s):  
Augustine O Ebonyi ◽  
Stephen Oguche ◽  
Ibrahim I Abok ◽  
Yetunde O Isa ◽  
Charles C Ani ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Line Probe Assay ◽  
Factors Associated ◽  
Probe Assay
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