scholarly journals Correlation between GenoType MTBDRplus Assay and Phenotypic Susceptibility Test for Prothionamide in Patients with Genotypic Isoniazid Resistance

2019 ◽  
Vol 82 (2) ◽  
pp. 143
Author(s):  
Joo Hee Lee ◽  
Kyung-Wook Jo ◽  
Tae Sun Shim
Keyword(s):  
Susceptibility Test ◽  
Isoniazid Resistance ◽  
Genotype Mtbdrplus ◽  
Phenotypic Susceptibility

scholarly journals Validation of the FluoroType MTBDR Assay for Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Complex Isolates

2018 ◽  
Vol 56 (6) ◽  
pp. e00072-18 ◽  
Author(s):  
Doris Hillemann ◽  
Carsten Haasis ◽  
Sönke Andres ◽  
Tobias Behn ◽  
Katharina Kranzer
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sanger Sequencing ◽  
High Sensitivity ◽  
Drug Susceptibility ◽  
Drug Susceptibility Testing ◽  
Molecular Diagnostic ◽  
Isoniazid Resistance ◽  
Content Type ◽  
Specificity And Sensitivity ◽  
Genotype Mtbdrplus

ABSTRACT For Mycobacterium tuberculosis complex (MTBC), the rapid and accurate diagnosis of drug resistance is crucial to ensure early initiation of appropriate therapy. Recently, a new molecular diagnostic test, the FluoroType MTBDR, aimed at detecting rifampin and isoniazid resistance has become available. This study aimed to evaluate the FluoroType MTBDR in comparison to phenotypic drug susceptibility testing (DST) using M. tuberculosis complex isolates. MTBC isolates underwent phenotypic DST and were tested using the FluoroType MTBDR and Genotype MTBDRplus. Sanger sequencing of the key regions of rpoB, katG, inhA, and aphC was performed for isolates with discordant phenotypic and molecular results. Furthermore, isolates with specific wild-type bands missing in the Genotype MTBDRplus, indicating the presence of a mutation, were investigated by Sanger sequencing. Specificity and sensitivity, defined as the proportions of isolates correctly determined as susceptible and resistant by the FluoroType MTBDR compared to phenotypic DST, were calculated. A total of 180 culture isolates were included; phenotypic DST showed 85 isolates susceptible to isoniazid and rifampin, 7 with isoniazid monoresistance, 7 with rifampin monoresistance, and 81 with multidrug resistance. The specificity of the FluoroType MTBDR was 100% (95% confidence interval [CI], 96.0 to 100%) for both rifampin and isoniazid. The sensitivity was 91.7% (95% CI, 83.6 to 96.6%) for isoniazid and 98.9% (95% CI, 93.8 to 100.0%) for rifampin. The FluoroType MTBDR has a high sensitivity and specificity for the detection of rifampin and isoniazid resistance when using culture isolates.

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