scholarly journals Association of Specific Immunoglobulin E to Staphylococcal Enterotoxin with Airway Hyperresponsiveness in Asthma Patients

2016 ◽  
Vol 79 (4) ◽  
pp. 295 ◽  
Author(s):  
Seong Han Kim ◽  
Seo Yeon Yang ◽  
Jihong You ◽  
Sang Bae Lee ◽  
Jin You ◽  
...  
Keyword(s):  
Airway Hyperresponsiveness ◽  
Immunoglobulin E ◽  
Staphylococcal Enterotoxin ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Asthma Patients

Role of local allergic inflammation and Staphylococcus aureus enterotoxins in Chinese patients with chronic rhinosinusitis with nasal polyps

2017 ◽  
Vol 131 (8) ◽  
pp. 707-713 ◽  
Author(s):  
K-J Cheng ◽  
Y-Y Xu ◽  
M-L Zhou ◽  
S-H Zhou ◽  
S-Q Wang
Keyword(s):  
Chronic Rhinosinusitis ◽  
Nasal Polyps ◽  
Immunoglobulin E ◽  
Allergic Inflammation ◽  
Staphylococcal Enterotoxin ◽  
Eosinophilic Chronic Rhinosinusitis ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Local Allergy

AbstractObjective:To investigate the role of local allergic inflammation and Staphylococcus aureus enterotoxins in chronic rhinosinusitis with nasal polyps.Methods:This study included 36 patients with chronic rhinosinusitis with nasal polyps and 18 controls. Total immunoglobulin E, eosinophil cationic protein, staphylococcal enterotoxin types A and B specific immunoglobulin E, staphylococcal enterotoxin types A and B, and myeloperoxidase levels were determined.Results:Four patients with chronic rhinosinusitis with nasal polyps had a local allergy. All chronic rhinosinusitis with nasal polyps patients tested negative for staphylococcal enterotoxin types A and B specific immunoglobulin E. The chronic rhinosinusitis with nasal polyps group had significantly elevated staphylococcal enterotoxin types A and B levels in the supernatant. Fourteen patients belonged to the eosinophilic chronic rhinosinusitis with nasal polyps group and the others were characterised as having non-eosinophilic chronic rhinosinusitis with nasal polyps.Conclusion:Local allergy may play a role in chronic rhinosinusitis with nasal polyps, independent of staphylococcal enterotoxin superantigens. Staphylococcal enterotoxins may be important in the pathogenesis of chronic rhinosinusitis with nasal polyps; however, their roles as superantigens were not confirmed in this study. In Chinese subjects, chronic rhinosinusitis with nasal polyps usually manifests as a neutrophilic inflammation.

scholarly journals Allergen diagnosis strategy: An experimental application of different methods in Guangzhou, Southern China

2021 ◽  
Vol 104 (1) ◽  
pp. 003685042199727
Author(s):  
Haisheng Hu ◽  
Zhifeng Huang ◽  
Wenting Luo ◽  
Xiangwei Zou ◽  
Hao Chen ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Southern China ◽  
High Sensitivity ◽  
Diagnostic Value ◽  
Scale Analysis ◽  
Strong Connection ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Asthma Patients ◽  
Clinical Conditions

Different methods have different diagnostic value under diverse clinical conditions. This study was conducted to analyze the clinical practical value of different assays in different situations and to establish the concept of an allergen diagnosis strategy. This study used Phadia CAP, Advan Sure (AdvS), Euroline Camera (ELC), and Allergy Lateral Flow Assay (ALFA) kits to detect allergen specific immunoglobulin E (sIgE) in the sera from 30 allergic asthma patients, with Phadia CAP as the gold standard. This study found high sensitivity for Dermatophagoides pteronyssinus in AdvS (92.6%), ELC (88.9%), and ALFA (85.2%), but poor sensitivity for F. domesticus in all three assays. Furthermore, the AdvS, ELC, and ALFA assays could detect 20, 21, and 8 allergens in one panel, respectively. The combination of the results showed that 93.3% and 46.7% of patients tested positive for ≥5 and ≥10 types of allergens, respectively. All patients who were tested positive for the Marine fish mixture were positive for both Shrimp and Crab. Similarly, patients who were tested positive for Chicken also tested positive for Pork. Optimal scale analysis showed a strong connection between Peanut, Soybean, Wheat, Japanese Hop, Western ragweed, and Oak allergens (Cronbach’s α = 91.1%). The ELC assay was more suitable for use in pollen-rich areas, whereas the ALFA assay was easy to establish in areas with poor medical conditions. An allergen diagnosis strategy needs to be considered for use in clinical practice.

The Role of Virus-specific Immunoglobulin E in Airway Hyperresponsiveness

2004 ◽  
Vol 170 (9) ◽  
pp. 952-959 ◽  
Author(s):  
Azzeddine Dakhama ◽  
Jung-Won Park ◽  
Christian Taube ◽  
Kosuke Chayama ◽  
Annette Balhorn ◽  
...  
Keyword(s):  
Airway Hyperresponsiveness ◽  
Immunoglobulin E ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

scholarly journals Allergic diseases attributable to atopy in a population sample of Asian children

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chao-Yi Wu ◽  
Hsin-Yi Huang ◽  
Wen-Chi Pan ◽  
Sui-Ling Liao ◽  
Man-Chin Hua ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Population Attributable Risk ◽  
Population Sample ◽  
International Study ◽  
Allergic Diseases ◽  
Population Based ◽  
School Age Children ◽  
Specific Immunoglobulin E ◽  
Asian Children ◽  
Specific Immunoglobulin

AbstractThe proportion of allergic diseases attributable to atopy remains a subject of controversy. This study aimed to estimate the population risk of physician-diagnosed asthma, rhinitis and eczema attributed to atopy among a population sample of Asian school-age children. Asian children aged 5–18 years (n = 1321) in the Prediction of Allergies in Taiwanese CHildren (PATCH) study were tested for serum allergen-specific immunoglobulin E. Physician-diagnosed asthma, rhinitis and eczema were assessed by a modified International Study of Asthma and Allergies in Childhood questionnaire. Atopy was defined as the presence of serum allergen-specific immunoglobulin E. In this population-based study, 50.4% of the subjects with asthma, 46.3% with rhinitis, and 46.7% with eczema were attributable to atopy. The population attributable risk (PAR) of atopy for three allergic diseases was higher in adolescents (asthma, 54.4%; rhinitis, 59.6%; eczema, 49.5%) than younger children aged less than 10 years (asthma, 46.9%; rhinitis, 39.5%; eczema, 41.9%). Among the seven allergen categories, sensitization to mites had the highest PARs for all three allergic diseases (51.3 to 64.1%), followed by sensitization to foods (asthma, 7.1%; rhinitis, 10.4%; eczema 27.7%). In conclusion, approximately half (46.3 to 50.4%) of Asian children in Taiwan with allergic diseases are attributable to atopy.

scholarly journals Effect of Specific Immunoglobulin E Response and Comorbidities on Effectiveness of MP-AzeFlu in a Real-Life Study

2020 ◽  
Vol 181 (10) ◽  
pp. 754-764
Author(s):  
Ludger Klimek ◽  
David Price ◽  
Gabriella Gálffy ◽  
Melanie Emmeluth ◽  
Arkady Koltun ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Real Life ◽  
Life Study ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Real Life Study

scholarly journals Total and Specific Immunoglobulin E and Their Relationship to Respiratory Symptoms in Quebec Children and Adolescents

2005 ◽  
Vol 12 (8) ◽  
pp. 426-432 ◽  
Author(s):  
Benoît Levesque ◽  
Jean-François Duchesne ◽  
Suzanne Gingras ◽  
Pierre Allard ◽  
Edgar Delvin ◽  
...  
Keyword(s):  
Children And Adolescents ◽  
Respiratory Symptoms ◽  
Immunoglobulin E ◽  
Geometric Mean ◽  
Allergic Sensitization ◽  
Population Sample ◽  
Ethnic Origin ◽  
Total Ige ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

BACKGROUND: Respiratory disease is a major cause of morbidity in young people. It is now recognized that atopy plays an important role in the development of chronic respiratory symptoms in children.OBJECTIVE: To examine the determinants and consequences of serum total and specific immunoglobulin E (IgE) in a general population sample of Québec children and adolescents.METHODS: In 1999, 2349 children and adolescents (nine, 13 or 16 years of age) who had participated in a respiratory symptom and disease questionnaire had their total IgE measured. Of these participants, a subsample of 451 children and adolescents was analyzed to detect antibodies to eight specific allergens (ie, allergens of dust mites [Dermatophagoides farinae and Dermatophagoides pteronyssinus], cat, dog, ragweed, Timothy grass, mould [alternaria] and cockroach).RESULTS: The geometric mean of the total IgE was 44.4 U/mL among all participants. Concentrations were higher in boys and increased with age. More than 41% of the participants were sensitized to at least one specific allergen. Such sensitization was strongly associated with the occurrence of respiratory conditions and symptoms, namely asthma, wheezing and rhinitis. Family history, school location and ethnic origin had an impact on the prevalence of atopy and total IgE levels.CONCLUSIONS: Allergic sensitization is a major determinant in the development of asthma, wheezing and rhinitis in children and adolescents in the province of Québec.

A Simple and Rapid Light-Initiated Chemiluminescence Assay for Quantitation of Artemisia-Specific Immunoglobulin E

2021 ◽  
pp. 1-8
Author(s):  
Dandan Liu ◽  
Bei Zhang ◽  
Lina Zhu ◽  
Lisheng Zheng ◽  
Shaoshen Li ◽  
...  
Keyword(s):  
Food Allergen ◽  
Clinical Guideline ◽  
Immunoglobulin E ◽  
Limit Of Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Chemiluminescence Assay ◽  
Limit Of Quantitation ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

<b><i>Background:</i></b> Light-initiated chemiluminescence assay (LICA) is a homogeneous assay that has been successfully used for the quantitation of food allergen-specific immunoglobulin E (sIgE), but not inhaled allergen-sIgE. Simultaneously, current assays used to detect allergen-sIgE are serum consuming and/or time consuming. Hence, we established a method for the quantitation of <i>Artemisia</i>-sIgE based on LICA and verified its performance according to the clinical guideline documents, laying a foundation for the quantitation of inhaled and food allergen-sIgE in parallel on LICA. <b><i>Methods:</i></b> The assay was established after optimizing the first incubation time and the dilutions of <i>Artemisia</i>-coated chemibeads, biotinylated goat anti-human IgE, and serum. In order to quantitate <i>Artemisia</i>-sIgE, the calibration curve was established with a high positive serum of known concentration. The assay performance was confirmed per the clinical guideline documents. In addition, the correlation between the results of LICA and capture enzyme-linked immunosorbent assay was evaluated. <b><i>Results:</i></b> The developed LICA’s coefficients of variation of repeatability and intermediate precision were 3.20%, 2.14%, and 3.85% and 4.30%, 4.00%, and 4.40%, respectively. The limit of detection was 0.10 kU<sub>A</sub>/L, and the limit of quantitation was 0.11 kU<sub>A</sub>/L. The range of linearity was from 0.27 kU<sub>A</sub>/L to 97.53 kU<sub>A</sub>/L (<i>r</i> = 0.9968). The correlation coefficient (<i>r</i>) for the correlation analysis between results of LICA and capture ELISA was 0.9087. This assay was successfully applied in 64 human serum samples, showing good sensitivity (82.20%) and specificity (100%). <b><i>Conclusion:</i></b> An <i>Artemisia</i>-sIgE quantitation assay based on LICA was successfully established. Its performance satisfied the clinical requirements and could be widely used in clinical laboratories.

Lidocaine anaphylaxis and lidocaine-specific immunoglobulin E measurement

2013 ◽  
Vol 1 (1) ◽  
pp. 98 ◽  
Author(s):  
Jin Yeop Yeo ◽  
Gyung Eun Kim ◽  
Ju Young Han ◽  
Jae Hyoung Im ◽  
Sung Wook Park ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin
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