Insecticidal effect of Bacillus pumilus PTB180 and Bacillus subtilis PTB185 used alone and in combination against the foxglove aphid and the melon aphid (Hemiptera: Aphididae)

2021 ◽  
pp. 1-15
Author(s):  
Mouna Kahia ◽  
Thi Thuy An Nguyen ◽  
Frédéric McCune ◽  
Rémi Naasz ◽  
Hani Antoun ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Pumilus ◽  
Insect Pests ◽  
Aphis Gossypii ◽  
Microbial Control ◽  
Insecticidal Effect ◽  
Foxglove Aphid ◽  
Melon Aphid ◽  
Microbial Agents ◽  
Better Than

Abstract The foxglove aphid, Aulacorthum solani (Kaltenbach) (Hemiptera: Aphididae), and the melon aphid, Aphis gossypii Glover (Hemiptera: Aphididae), are among the serious insect pests found in greenhouses. The efficacy of microbial control against these insects has been demonstrated and can be enhanced by the combination of different microbial agents. This study evaluated the efficacy of Bacillus pumilus Meyer and Gottheil PTB180 and Bacillus subtilis (Ehrenberg) Cohn PTB185, used alone and together, to control these two aphids both in the laboratory and in greenhouse on tomato, Solanum lycopersicum Linnaeus (Solanaceae), and cucumber, Cucumis sativus Linnaeus (Cucurbitaceae), plants. The results from the laboratory tests showed an increase in mortality induced by all biological treatments. In the greenhouse, all treatments induced mortality rates significantly higher than that of the control for A. solani. Similarly, all treatments performed better than the control against A. gossypii, significantly reducing its reproduction. Furthermore, we found no additive effects when mixing products nor negative interactions affecting survival for the bacteria investigated. These microorganisms therefore have potential for use in biological control.

scholarly journals Aphids species on potato crops in Canterbury

2001 ◽  
Vol 54 ◽  
pp. 235-239 ◽  
Author(s):  
M.A.W. Stufkens ◽  
D.A.J. Teulon
Keyword(s):  
Green Peach Aphid ◽  
Aphis Gossypii ◽  
Leaf Roll ◽  
Macrosiphum Euphorbiae ◽  
Potato Leaf ◽  
Potato Aphid ◽  
Potato Viruses ◽  
Foxglove Aphid ◽  
Melon Aphid ◽  
Potato Crops

A survey of aphids infesting table processing and seed potato crops was undertaken in Canterbury during the 19992000 season (November March) The green peach aphid (Myzus persicae (Sulzer)) was the most common aphid found on potato plants (>85 of all aphids) followed by the foxglove aphid (Aulacorthum solani (Kaltenbach)) and the potato aphid (Macrosiphum euphorbiae (Thomas)) These three aphids are vectors of the potato leaf roll virus (PLRV) and other potato viruses A further eight species were found in low numbers including five species that are vectors of potato viruses (not PLRV) The melon aphid (Aphis gossypii Glover) was found in sizable numbers on sprayed potatoes in the Hawkes Bay This species which is known to have developed resistance to some insecticides on other crops in New Zealand was found in only low numbers in three crops in Canterbury

scholarly journals Efficacy of different entomopathogenic fungal isolates against chilli aphid, Myzus Persicae (Sulz.)

2021 ◽  
Vol 23 (09) ◽  
pp. 241-247
Author(s):  
S. Sumaiya Parveen ◽  
◽  
R. Philip Sridhar ◽  
S. Usha Nandhini ◽  
◽  
...  
Keyword(s):  
Myzus Persicae ◽  
Metarhizium Anisopliae ◽  
Field Experiments ◽  
Insect Pests ◽  
Microbial Control ◽  
Minimal Impact ◽  
Lecanicillium Lecanii ◽  
Fungal Isolates ◽  
Species Specific ◽  
Microbial Agents

In recent years, microbial control of insect pests is becoming popular as insect pathogens such as bacteria, viruses, fungi and nematodes serve as potential bioagents in pest management. Among the different microbial agents, entomopathogenic fungi (EPF) are gaining importance in pest control. They can be easily mass cultured on artificial media without affecting their virulence at a cheaper cost. They are highly species specific with minimal impact on non-target organisms. The current study aimed to study the efficacy of entomopathogenic fungal isolates against aphid, Myzus Persicae (Sulz.) in chilli. Laboratory and field experiments were conducted to evaluate the pathogenicity of Beauveria bassiana, Metarhizium anisopliae and Lecanicillium lecanii against chilli aphid. From the study it is revealed that B. bassiana @ 108 spores ml-1 were found to be effective and found to be more superior to the other entomopathogenic fungal isolates viz., Metarhizium anisopliae and Lecanicillium lecanii against chilli aphid.

Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45

1995 ◽  
Vol 78 (2) ◽  
pp. 97-108 ◽  
Author(s):  
C. Leifert ◽  
H. Li ◽  
Siripun Chidburee ◽  
S. Hampson ◽  
Suzanne Workman ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Pumilus ◽  
Antibiotic Production ◽  
Biocontrol Activity

Immobilizing Bacillus subtilis on the carrier of poly (acrylic acid)/sodium bentonite for treating sludge from Pangasius fish ponds

2021 ◽  
Vol 6 (1) ◽  
pp. 43-49
Author(s):  
Thanh Duoc Nguyen ◽  
Binh Doan ◽  
Thu Hong Pham Thi
Keyword(s):  
Bacillus Subtilis ◽  
Acrylic Acid ◽  
Absorbed Dose ◽  
Pond Water ◽  
Reaction Yield ◽  
Fish Ponds ◽  
Adsorption Method ◽  
Sodium Bentonite ◽  
Better Than ◽  
Poly Acrylic Acid

Sodium bentonite (NaBent) was modified by poly(acrylic acid) (PAAc) to prepare the carriers for immobilization of Bacillus subtilis. Different mixtures of NaBent/AAc were regularly dispersed in distilled water and irradiated under gamma rays at an absorbed dose of 6.5 kGy with dose rate of 0.85 kGy/hr in air for polymerization of acrylic acid and formation of poly(acrylic acid)/sodium bentonite (PAAc–NaBent). The reaction yield was determined with the initial concentration of acrylic acid (AAc). The functional group properties of the resulting PAAc–NaBent were analyzed by Fourier Transform Infrared spectra (FTIR). Bacillus subtilis cells were immobilized on both NaBent and PAAc–NaBent as carriers by adsorption method for treating the sludge contaminated by fish feces and residual feed from the Pangasius farming ponds. The results showed that immobilization capacity of Bacillus subtilis on the PAAc–NaBent was better than that on non-modified NaBent. Analysis of BOD for the farming pond water containing Bacillus subtilis and the bacteria immobilized carriers with time revealed the lower BOD values obtained with the samples containing PAAc–NaBent, suggested that degradation of organic pollutants by Bacillus subtilis immobilized on the PAAc–NaBent was faster than that by free bacteria.

scholarly journals The Role of Soil Amendments on Population Dynamics of Insect Pests, Growth Parameters and Yield of Eggplant, Solanum melongena (L.) Moench

2017 ◽  
Vol 7 (1) ◽  
pp. 7
Author(s):  
P. K. Baidoo ◽  
M. B. Mochiah ◽  
D. Asare ◽  
A. A. Sefah
Keyword(s):  
Soil Fertility ◽  
Plant Height ◽  
Bemisia Tabaci ◽  
Insect Pests ◽  
Soil Amendments ◽  
Aphis Gossypii ◽  
Solanum Melongena ◽  
Cow Dung ◽  
Leucinodes Orbonalis ◽  
Number Of Leaves

Loss of soil fertility as a result of continuous cropping on the same piece of land has necessitated the need to improve soil fertility for better crop yields. Inorganic and organic fertilizers have been used to improve soil fertility, however, excessive use of soil amendments improve vegetative growth of plants thereby attracting large numbers of insect pests. Cow dung and poultry droppings were used as soil amendments in a field experiment using eggplant Solanum melongena. The effects of these organic manures were compared with inorganic fertilizer (NPK) and a control where there was no application of soil amendment in a randomized complete block design with 3 replicates. Parameters studied were pests’ and their numbers, plant height, number of leaves per plant, leaf area, stem girth and yield. The major insect pests identified on the plant were Bemisia tabaci, Aphis gossypii, Leucinodes orbonalis and Eublemma olivacea. Bemisia tabaci and Aphis gossypii scores were significantly larger on cow dung and poultry manure plots. Leucinodes orbonalis and Eublemma olivacea numbers were not significantly different on the treated and control plots. Mean plant height, number of leaves and yield differed significantly among the soil amended plots. Even though soil amendments improve the nutrient content of the soil and the yield of crops it could lead to increase in pests numbers and damage caused to plants.

scholarly journals Fine-Grained Pests Recognition Based on Truncated Probability Fusion Network via Internet of Things in Forestry and Agricultural Scenes

Algorithms ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 290
Author(s):  
Kai Ma ◽  
Ming-Jun Nie ◽  
Sen Lin ◽  
Jianlei Kong ◽  
Cheng-Cai Yang ◽  
...  
Keyword(s):  
Internet Of Things ◽  
Insect Pests ◽  
Accurate Identification ◽  
Technical Application ◽  
Network Mining ◽  
Fine Grained ◽  
Feature Expression ◽  
The Internet Of Things ◽  
Different Levels ◽  
Better Than

Accurate identification of insect pests is the key to improve crop yield and ensure quality and safety. However, under the influence of environmental conditions, the same kind of pests show obvious differences in intraclass representation, while the different kinds of pests show slight similarities. The traditional methods have been difficult to deal with fine-grained identification of pests, and their practical deployment is low. In order to solve this problem, this paper uses a variety of equipment terminals in the agricultural Internet of Things to obtain a large number of pest images and proposes a fine-grained identification model of pests based on probability fusion network FPNT. This model designs a fine-grained feature extractor based on an optimized CSPNet backbone network, mining different levels of local feature expression that can distinguish subtle differences. After the integration of the NetVLAD aggregation layer, the gated probability fusion layer gives full play to the advantages of information complementarity and confidence coupling of multi-model fusion. The comparison test shows that the PFNT model has an average recognition accuracy of 93.18% for all kinds of pests, and its performance is better than other deep-learning methods, with the average processing time drop to 61 ms, which can meet the needs of fine-grained image recognition of pests in the Internet of Things in agricultural and forestry practice, and provide technical application reference for intelligent early warning and prevention of pests.

scholarly journals Potentials of the Extracts of Algerian Saharan Plant Cotula cinerea for the Management of Two Insect Pests, Aphis fabae and Tribolium castaneum

2020 ◽  
Vol 15 (2) ◽  
pp. 41-57
Author(s):  
Fatma Acheuk ◽  
◽  
Kemais Abdellaoui ◽  
Wassima Lakhdari ◽  
Nora Chahbar ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Insect Pests ◽  
Ethanolic Extract ◽  
Aphis Fabae ◽  
Insect Species ◽  
Contact Toxicity ◽  
Phytochemical Study ◽  
Insecticidal Effect ◽  
Cotula Cinerea ◽  
Synergetic Action

The insecticidal potential of the Saharan plant Cotula cinerea, was evaluated on two insect species namely Aphis fabae and Tribolium castaneum by topical application (contact toxicity) and repellency test. A crude ethanolic extract of aerial part of the plant was prepared and tested in the laboratory on adults of both species. For contact toxicity, five doses were tested on each of the two species 1.56, 3.12, 6.25, 12.5 and 25 mg/ml for A. fabae and 25, 50, 250, 350 and 500 µg/insect for T. castaneum. The repellency of the extract was studied at the dose 500 μg/insect for T. castaneum and 25 µg/ml for A. fabae. Results showed that the repellency of the extract increased with exposure time and the highest rates were observed after 4 h of exposure (72.33 ± 22% for T. castaneum and 87 ± 3.6% for A. fabae). For insecticidal activity, at the highest doses (25 mg/ml and 500 µg/ml), 100% mortality is obtained 72 h after treatment for A. fabae and after 48 h for T. castaneum. The extract of this plant was found to be more toxic against T. castaneum adults. LD50 calculated 24 h after treatment for the two species is estimated at 1.7 mg/ml for A. fabae and at 30.3 µg/insect for T. castaneum. The extract of this plant inhibited the activity of acetylcholinesterase (AChE) in both insect species. This result suggests that this plant has a neurotoxic effect on A. fabae and T. castaneum. The results of phytochemical study showed that the plant is mainly rich in flavonoids, gallic tannins, alkaloids, saponosides and glucosides. The insecticidal effect obtained in this study could be due to the synergetic action of all constituents of the extract. Results suggest the possibility of using the extracts of this plant in integrated pest management to replace the chemical insecticides.

Expression of a xylanase gene of Bacillus pumilus in Escherichia coli and Bacillus subtilis

1985 ◽  
Vol 22 (4) ◽  
Author(s):  
Watanalai Panbangred ◽  
Eiichiro Fukusaki ◽  
EvangelineC. Epifanio ◽  
Atsuhiko Shinmyo ◽  
Hirosuke Okada
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Bacillus Pumilus ◽  
Xylanase Gene
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