Host-range testing of a mixture of two nucleopolyhedroviruses of Choristoneura fumiferana (Lepidoptera: Tortricidae)

2011 ◽  
Vol 143 (2) ◽  
pp. 165-177 ◽  
Author(s):  
William J. Kaupp ◽  
Kevin N. Barber ◽  
William E. Fick ◽  
Peter M. Ebling ◽  
Tim R. Ladd ◽  
...  
Keyword(s):  
Host Range ◽  
Choristoneura Fumiferana ◽  
Cydia Pomonella ◽  
Mortality Rates ◽  
Choristoneura Rosaceana ◽  
Adult Males ◽  
Significant Treatment ◽  
Choristoneura Pinus ◽  
Choristoneura Pinus Pinus ◽  
Primer Sets

AbstractThe host range of a mixture of Choristoneura fumiferana (Clemens) nucleopolyhedroviruses (CfMNPV and CfDefNPV) was investigated using a per os bioassay of larvae of 29 species of Lepidoptera and adult males of Megachile rotundata (F.) (Hymenoptera: Megachilidae). Using a whole-genomic DNA probe, positive results were obtained in 8 of 10 Tortricidae: Archips cerasivorana (Fitch), Choristoneura fractivittana (Clemens), C. fumiferana, Choristoneura occidentalis Freeman, Choristoneura pinus pinus Freeman, Choristoneura rosaceana (Harris), Clepsis persicana (Fitch), and Cydia pomonella (L.); one Crambidae: Ostrinia nubilalis (Hübner); one arctiine Erebidae: Estigmene acrea (Drury); and two Noctuidae: Oligia illocata (Walker) and Pyrrhia exprimens (Walker). Mortality rates were highest among C. fumiferana, C. occidentalis, C. pinus pinus, A. cerasivorana, and C. pomonella. Sequenced polymerase chain reaction (PCR) amplicons from infected individuals from several species confirmed that the primer sets amplified the target viruses. CfMNPV was consistently found in virus-fed C. fumiferana; whereas, CfDefNPV was present only occasionally. The presence of CfMNPV and CfDefNPV in A. cerasivorana was confirmed by PCR and DNA sequencing. Significant treatment-mortality rates were induced in the noctuids P. exprimens and Acronicta impleta Walker; PCR determined that both viruses were present in treated P. exprimens but only CfMNPV was present in A. impleta. No virus was detected in M. rotundata.

FEEDING BEHAVIOUR OF THE FIRST-INSTAR CHORISTONEURA FUMIFERANA AND CHORISTONEURA PINUS PINUS (LEPIDOPTERA: TORTRICIDAE)

1999 ◽  
Vol 131 (1) ◽  
pp. 79-84 ◽  
Author(s):  
A. Retnakaran ◽  
W.L. Tomkins ◽  
M.J. Primavera ◽  
S.R. Palli
Keyword(s):  
Fluorescent Protein ◽  
Choristoneura Fumiferana ◽  
Glass Tube ◽  
Choristoneura Pinus ◽  
Jack Pine Budworm ◽  
Occlusion Bodies ◽  
Early Instar ◽  
Choristoneura Pinus Pinus ◽  
Green Fluorescent ◽  
Red Dye

AbstractWe have discovered that, contrary to the long-held belief, 1st-instar spruce budworm, Choristoneura fumiferana Clemens, do feed. They display red alimentary tracts if they are provided with diet containing the red dye amaranth. They graze on the surface of balsam fir needles sprayed with rhodamine and ingest the fluorescent material, which can be detected in the frass pellets deposited inside the hibernacula. When emerging 1st instars were allowed to crawl on the inside surface of a glass tube coated with the polyhedral inclusion bodies of a recombinant C. fumiferana virus containing the gene for the green fluorescent protein, the larvae showed the characteristic green fluorescence, indicating that not only had they ingested the occlusion bodies but also the virus had replicated and infected different tissues. Similar results were obtained with the jack pine budworm, Choristoneura pinus pinus Freeman, which has an identical life history. The advantages of early-instar intervention to minimize defoliation by using control agents such as the ecdysteroid agonist, tebufenozide (RH-5992, Mimic® formulation), are discussed.

Differences in trapping mortality rates of northern flying squirrels

1993 ◽  
Vol 71 (3) ◽  
pp. 660-663 ◽  
Author(s):  
Daniel K. Rosenberg ◽  
Robert G. Anthony
Keyword(s):  
Mortality Rates ◽  
Differential Mortality ◽  
Glaucomys Sabrinus ◽  
Adult Males ◽  
Extrinsic Factors ◽  
Age Cohorts ◽  
Flying Squirrels ◽  
Northern Flying Squirrel ◽  
Northern Flying Squirrels ◽  
Juvenile Males

We described trapping mortality rates of northern flying squirrel (Glaucomys sabrinus) populations in western Oregon, U.S.A., and evaluated the effects of sex, age, body mass, and number of times an individual was recaptured on these rates. Although the overall trapping mortality rates were relatively low (7%) during 16–21 day trapping sessions, we observed differential mortality rates among the sex and age cohorts. The order of mortality rates was: juvenile females (32.3%) > juvenile males (11.1%) > adult females (5.1%) = adult males (4.1%). Overall trapping mortality rates were not affected by the number of times an individual was captured. We hypothesize that the differences we found were due to extrinsic factors (weather-related) acting on differential behavioral responses to trapping and thresholds of stress an animal can tolerate.

High-Throughput Sequencing for Life-History Sorting and for Bridging Reference Sequences in Marine Gerromorpha (Insecta: Heteroptera)

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Jia Jin Marc Chang ◽  
Yin Cheong Aden Ip ◽  
Lanna Cheng ◽  
Ismael Kunning ◽  
Ralph R Mana ◽  
...  
Keyword(s):  
Life History ◽  
High Throughput Sequencing ◽  
Association Studies ◽  
Morphological Characters ◽  
Adult Males ◽  
Accurate Identification ◽  
Life History Stages ◽  
Coi Sequences ◽  
Primer Sets ◽  
Insect Taxonomy

Abstract Accurate identification and association of larval specimens with adults is a major challenge in insect taxonomy. Fortunately, it is now possible for nonexperts to sort collections of bulk samples with DNA barcodes rapidly and cost-effectively. We demonstrate this process using nanopore barcoding of 757 marine insects (Insecta: Gerromorpha), of which 81% were nymphs and many samples did not have co-occurring adult males for specific identification. We successfully associated 738 specimens (97%) to nine gerromorphan species, which would have been impossible to identify using morphological characters alone. This improved ability to incorporate information from all life-history stages has led to greater precision of species distributional ranges—knowledge that will be crucial for a more complete understanding of marine insects. We also highlighted two distinct, nonoverlapping Gerromorpha COI sequence databases on GenBank—a consequence of using two different primer sets to amplify different regions of COI. This issue inevitably hinders species identification with DNA-based methods, particularly for poorly represented groups such as marine insects. We bridged these databases by analyzing full-length COI sequences. We believe this will inspire future studies to incorporate DNA-based methods for more adult–larval association studies and for enhancing existing genetic resources, especially in understudied groups.

scholarly journals Development of the Automated Primer Design Workflow Uniqprimer and Diagnostic Primers for the Broad-Host-Range Plant Pathogen Dickeya dianthicola

Plant Disease ◽  
2019 ◽  
Vol 103 (11) ◽  
pp. 2893-2902 ◽  
Author(s):  
Shaista Karim ◽  
R. Ryan McNally ◽  
Afnan S. Nasaruddin ◽  
Alexis DeReeper ◽  
Ramil P. Mauleon ◽  
...  
Keyword(s):  
Host Range ◽  
Bacterial Pathogen ◽  
The United States ◽  
Broad Host Range ◽  
Software Pipeline ◽  
Widespread Occurrence ◽  
Field Samples ◽  
Primer Sets ◽  
Genome Analyses ◽  
User Friendly

Uniqprimer, a software pipeline developed in Python, was deployed as a user-friendly internet tool in Rice Galaxy for comparative genome analyses to design primer sets for PCRassays capable of detecting target bacterial taxa. The pipeline was trialed with Dickeya dianthicola, a destructive broad-host-range bacterial pathogen found in most potato-growing regions. Dickeya is a highly variable genus, and some primers available to detect this genus and species exhibit common diagnostic failures. Upon uploading a selection of target and nontarget genomes, six primer sets were rapidly identified with Uniqprimer, of which two were specific and sensitive when tested with D. dianthicola. The remaining four amplified a minority of the nontarget strains tested. The two promising candidate primer sets were trialed with DNA isolated from 116 field samples from across the United States that were previously submitted for testing. D. dianthicola was detected in 41 samples, demonstrating the applicability of our detection primers and suggesting widespread occurrence of D. dianthicola in North America.

Association of within-tree jack pine budworm feeding patterns with canopy level and within-needle variation of water, nutrient, and monoterpene concentrations

1998 ◽  
Vol 28 (2) ◽  
pp. 228-233 ◽  
Author(s):  
Kimberly F Wallin ◽  
Kenneth F Raffa
Keyword(s):  
Pinus Banksiana ◽  
Jack Pine ◽  
Larval Feeding ◽  
Feeding Patterns ◽  
Choristoneura Pinus ◽  
Jack Pine Budworm ◽  
Choristoneura Pinus Pinus ◽  
Zinc Levels ◽  
Calcium Iron ◽  
Relative Differences

The possibility that uneven within-tree feeding patterns by jack pine budworm (Choristoneura pinus pinus Freeman) larvae could be related to underlying variation in host jack pine (Pinus banksiana Lamb.) foliar water, nutrient, and monoterpene contents was considered. Choristoneura pinus pinus feeds disproportionately in the upper portion of the canopy and almost exclusively on the basal portions of needles. Within needles, the distribution of water, several nutrients, and monoterpenes varied significantly between the distal and basal sections. Water, nitrogen, sulfur, manganese, and zinc levels occurred in higher concentrations in the distal section of the needle, which would not be predicted based on C. pinus pinus feeding patterns. Phosphorus, potassium, calcium, iron, and copper were significantly higher in the basal section. Although the latter differences might be predicted based on C. pinus pinus feeding patterns, they were not strong (ranging from 7.8% to 36.4% relative differences, as compared with 10.7%-50.0% relative differences in the former group). By contrast, concentrations of foliar monoterpenes were more strongly associated with known feeding patterns of C. pinus pinus. These relative differences ranged from myrcene (26.8%) to limonene (44.79%). Thus, foliar differences affecting within-needle feeding selection appear more associated with allelochemicals than nutrients or water. In contrast with the within-needle associations between larval feeding and foliar chemistry, no associations between upper and lower canopy foliage were observed. Water, nutrients, and monoterpenes were evenly distributed across the upper and lower canopy locations. Thus, differential feeding between canopy locations cannot be explained by foliar constituents. Rather, it is more likely explained by other environmental factors, such as proximity to and density of reproductive and vegetative shoots.

scholarly journals Malnutrition Rates in Chile from the Nitrate Era to the 1990s

2021 ◽  
Vol 18 (24) ◽  
pp. 13112
Author(s):  
Manuel Llorca-Jaña ◽  
Diego Barría Traverso ◽  
Diego del Barrio Vásquez ◽  
Javier Rivas
Keyword(s):  
Developed Countries ◽  
Mortality Rates ◽  
Adult Males ◽  
Sharp Decline ◽  
Low Levels ◽  
The 1960S ◽  
Chilean Population ◽  
Very High ◽  
Over Time

Following Salvatore and the WHO, in this article, we provide the first long-term estimates of malnutrition rates for Chile per birth cohort, measured through stunting rates of adult males born from the 1870s to the 1990s. We used a large sample of military records, representative of the whole Chilean population, totalling over 38 thousand individuals. Our data suggest that stunting rates were very high for those born between the last three decades of the nineteenth century and the first two decades of the twentieth century. In addition, stunting rates increased from the 1870s to the 1900s. Thereafter, there was a clear downward trend in stunting rates (despite some fluctuations), reaching low levels of malnutrition, in particular, from the 1960s (although these are high if compared to developed countries). The continuous decrease in stunting rates from the 1910s was mainly due to a combination of factors, the importance of which varied over time, namely: Improved health (i.e., sharp decline in infant mortality rates during the whole period); increased energy consumption (from the 1930s onwards, but most importantly during the 1990s); a decline in poverty rates (in particular, between the 1930s and the 1970s); and a reduction in child labour (although we are less able to quantify this).

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