DNA fingerprinting of tabanids (Diptera: Tabanidae) and their respective egg masses using PCR – restriction fragment profiling

2004 ◽  
Vol 136 (5) ◽  
pp. 605-619 ◽  
Author(s):  
M. Iranpour ◽  
A.M. Schurko ◽  
G.R. Klassen ◽  
T.D. Galloway
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Rna ◽  
Intergenic Spacer ◽  
Interspecific Variation ◽  
Egg Masses ◽  
Polymerase Chain ◽  
High Level ◽  
Rna Genes ◽  
Profiling Analysis ◽  
Respective Species

AbstractPolymerase chain reaction and subsequent restriction fragment profiling analysis were used to associate collected tabanid egg masses with their respective species of adult horse flies and deer flies (Diptera: Tabanidae) in Manitoba, Canada. The ribosomal DNA (rDNA) intergenic spacer between the 28S and 18S ribosomal RNA genes was used successfully to differentiate 34 species of adult tabanids representing five genera: Atylotus (1 sp.), Chrysops (10 spp.), Haematopota (1 sp.), Hybomitra (17 spp.), and Tabanus (5 spp.). rDNA was a suitable molecular target for identifying tabanid species because of the high level of interspecific variation when comparing fragment profiles among different species, and the corresponding minimal intraspecific variation among individuals of the same species. Restriction fragment profiles from 56 field-collected tabanid egg masses were compared with those previously obtained from adults of known species. Egg masses of five species were identified: Hybomitra lasiophthalma (Macquart), Hybomitra nitidifrons nuda (McDunnough), Chrysops aestuans van der Wulp, Chrysops excitans Walker, and Chrysops mitis Osten Sacken. We also provide physical descriptions of these tabanid egg masses along with pictures.

The Nor-D3 locus of Triticum tauschii: natural variation and genetic linkage to markers in chromosome 5

Genome ◽  
1991 ◽  
Vol 34 (3) ◽  
pp. 387-395 ◽  
Author(s):  
E. S. Lagudah ◽  
R. Appels ◽  
D. McNeil
Keyword(s):  
Ribosomal Rna ◽  
Intergenic Spacer ◽  
D Genome ◽  
Triticum Tauschii ◽  
Intergenic Spacer Region ◽  
Grain Softness ◽  
High Level ◽  
Rna Genes ◽  
Rdna Polymorphism ◽  
Grain Softness Protein

Variation in the intergenic spacer region of the ribosomal RNA genes (located at the Nor locus) was assayed in a collection of 411 accessions of Triticum tauschii from Turkey, USSR, Iran, Afghanisan, Pakistan, and China. Twenty rDNA genotypes were identified and it was demonstrated that T. tauschii accessions from the USSR and Iran have the highest diversity at the Nor locus. At least four of the rDNA genotypes were demonstrated to be alleles of a single major locus, in segregating F2 progeny analyses. The TaqI restriction fragment associated with rDNA genotype 7 was shown to be the same as the Nor-D3a allele present in all bread wheats (based on chromosome location and length of the intergenic spacer region). This genotype was significantly associated with T. t. ssp. strangulata, previously argued to be the donor of the D genome to hexaploid wheat. The Nor locus showed a high level of recombination with the 5SDna-2 locus, which was also located on chromosome 5D. The Nor locus is placed distal to the 5SDna-2 locus but proximal to the grain softness protein gene (XGsp) on the short arm of chromosome 5D.Key words: D genome, Nor-D3, rDNA polymorphism, chromosomal location.

Molecular evolution of the intergenic spacer in the nuclear ribosomal RNA genes of Cucurbitaceae

1993 ◽  
Vol 36 (2) ◽  
pp. 144-152 ◽  
Author(s):  
Klaus King ◽  
Ramon A. Torres ◽  
Ulrike Zentgraf ◽  
Vera Hemleben
Keyword(s):  
Molecular Evolution ◽  
Ribosomal Rna ◽  
Intergenic Spacer ◽  
Ribosomal Rna Genes ◽  
Rna Genes

Identification of Armillaria species isolated from bigtooth aspen based on rDNA RFLP analysis

1998 ◽  
Vol 28 (1) ◽  
pp. 141-149 ◽  
Author(s):  
T M Frontz ◽  
D D Davis ◽  
B A Bunyard ◽  
D J Royse
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Rna ◽  
Rflp Analysis ◽  
Rrna Gene ◽  
Polymorphism Analysis ◽  
5S Rrna Gene ◽  
Aspen Tree ◽  
Armillaria Gallica ◽  
Polymerase Chain ◽  
Armillaria Species

Restriction fragment length polymorphism analysis (RFLP) of the intergenic region (IGR-1) between the 3 ' end of the 26S ribosomal RNA gene and the 5 ' end of the 5S rRNA gene was used to identify 39 isolates of Armillaria species collected from live or recently dead bigtooth aspen (Populus grandidentata Michx.) trees and sucker sprouts in the Tioga State Forest, Pennsylvania. The unknown isolates were identified by comparing their restriction fragment patterns with 18 isolates of known Armillaria species common to the northeastern United States. Twenty of the unknown isolates (50%) were identified as either Armillaria gallica or Armillaria calvescens. Eighteen (46%) of the isolates were identified as Armillaria ostoyae. One isolate of Armillaria sinapina was obtained from a recently dead aspen tree. One isolate of Armillaria mellea, considered to be the most divergent of the Armillaria species, was obtained from basidiomes fruiting on a recently dead aspen tree near Berwick, Pennsylvania. In some instances, amplification of DNA was possible by adding mycelial scrapes directly to the polymerase chain reaction (PCR) mix, thus precluding the need for DNA extraction. Advancements in RFLP analysis may offer a method able to provide rapid and precise identification of most North American and European Armillaria isolates.

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