FOLIAR AND SYSTEMIC APPLICATIONS OF NEEM SEED EXTRACT FOR CONTROL OF SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (CLEM.) (LEPIDOPTERA: TORTRICIDAE), INFESTING BLACK AND WHITE SPRUCE SEED ORCHARDS

1997 ◽  
Vol 129 (4) ◽  
pp. 645-655 ◽  
Author(s):  
Kevin W. Wanner ◽  
Blair V. Helson ◽  
Barry C. Kostyk
Keyword(s):  
Black Spruce ◽  
Choristoneura Fumiferana ◽  
Larval Mortality ◽  
Spruce Budworm ◽  
Weather Conditions ◽  
Urban Environments ◽  
Seed Extract ◽  
Neem Seed ◽  
Adverse Weather ◽  
Neem Seed Extract

AbstractNeem seed extract (20, 60, and 180 ppm azadirachtin) applied to the crowns of black spruce trees immediately prior to female reproductive bud flush, resulted in high mortality of early instar spruce budworm larvae. Dose-dependent reductions of 40–93%, as compared with check trees, were observed 2 and 4.5 weeks after treatment. Seed cone protection from grazing damage, however, was moderate; at 60 ppm azadirachtin, the incidence of cone grazing was reduced 29%, and the proportion of severely grazed cones reduced 39%, as compared with check trees. Larval mortality and bud damage observed in the laboratory corroborated field results. High toxicity to second-instar larvae, and performance during adverse weather conditions, supports the potential of neem seed extract for control of spruce budworm defoliation. However, both laboratory and field results indicate that feeding occurs before the effects of neem seed extract manifest, resulting in moderate protection of cones. Whereas foliage can tolerate low levels of feeding, female strobili are damaged significantly. Excellent protection of foliage was achieved from systemic injections of neem seed extract to white and black spruce trees (1.5 g azadirachtin/tree). Neem seed extract, formulated for systemic application, could provide a botanical alternative for control of forest pests in urban environments.

scholarly journals Control of Insect Damage to Black Spruce Seed Cones with Neem

1997 ◽  
Vol 14 (1) ◽  
pp. 40-43 ◽  
Author(s):  
Barry C. Kostyk ◽  
Kevin W. Wanner
Keyword(s):  
Black Spruce ◽  
Spruce Budworm ◽  
Female Flower ◽  
Seed Extract ◽  
Insect Damage ◽  
Neem Seed ◽  
Flower Abortion ◽  
Lepidopteran Larvae ◽  
Two Stages ◽  
Neem Seed Extract

Abstract A neem seed extract was applied to black spruce trees at two stages of reproductive development to control insect damage to the seed cones. Applied after pollination, neem had no effect on damage by the spruce cone maggot but did provide some protection against defoliation by the spruce budworm. Neem had no effect on the numbers of spruce budworm larvae for the first 9 days of the experiment, but after 23 days, populations declined on trees with two of three neem treatments compared to control trees. Neem reduced cone grazing damage by lepidopteran larvae when applied during the flowering stage. However, increased female flower abortion also resulted. The application of neem seed extract for controlling spruce budworm damage appears promising, but more information is needed on earlier applications in the field to determine if this product could be used operationally without causing substantial flower abortion. North. J. Appl. For. 14(1):40-43.

scholarly journals Effect of aqueous neem seed extract via irrigation on larvae of Liriomyza sativae in melon crop

2018 ◽  
Vol 36 (3) ◽  
pp. 353-356 ◽  
Author(s):  
Ewerton M Costa ◽  
Francisco EL Silva ◽  
Elton L Araujo
Keyword(s):  
Larval Mortality ◽  
Total Mortality ◽  
Seed Extract ◽  
Neem Seed ◽  
Liriomyza Sativae ◽  
Randomized Design ◽  
Soil Irrigation ◽  
Completely Randomized Design ◽  
Pupal Mortality ◽  
Neem Seed Extract

ABSTRACT The leafminer [Liriomyza sativae (Diptera: Agromyzidae)] is one of the main pests of melon crop (Cucumis melo) in the states of Rio Grande do Norte and Ceará. Thus, adopting strategies to control this destructive insect is essential. The aim of this study was to evaluate the effect of aqueous neem seed extract, applied to soil via irrigation, on leafminer larvae, in the melon crop. An experiment was conducted in greenhouse, in completely randomized design, consisting of five treatments (four doses of aqueous neem seed extract: 1; 3; 5 and 10 g/100 mL) and the control consisting only of distilled water and 10 replicates (melon plants). The treatments were applied in a single dose, via soil irrigation. In the first stage of the evaluation, 24 hours after application, the number of dead larvae per plant was counted. In the second step, pupal mortality was recorded. The total mortality was also calculated considering the number of larvae and the number of emerged adults. We verified that, increasing the concentration of aqueous neem seed extract, an increase in the larval and pupal mortality of the leafminer in melon crop was noticed. The concentration 10 g/100 mL presented the best results, resulting in 36.4% of larval mortality, 96.5% of pupal mortality and 96.8% of total mortality.

LONG TERM STUDY OF THE EFFECTIVENESS OF AERIAL APPLICATION OF BACILLUS THURINGIENSIS – ACEPHATE COMBINATIONS AGAINST THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)

1977 ◽  
Vol 109 (9) ◽  
pp. 1239-1248 ◽  
Author(s):  
O. N. Morris
Keyword(s):  
Bacillus Thuringiensis ◽  
Choristoneura Fumiferana ◽  
Larval Mortality ◽  
Abies Balsamea ◽  
Spruce Budworm ◽  
White Spruce ◽  
Balsam Fir ◽  
Population Densities ◽  
Long Term Study

AbstractBacillus thuringiensis (Dipel® 36B) mixed with a sublethal concentration of acephate (Orthene®) (O, S-dimethyl acetylphosphoramidothioate), an organophosphorous insecticide, was applied at 2.35–14 l./ha to white spruce (Picea glauca) and balsam fir (Abies balsamea) trees infested with spruce budworm, Choristoneura fumiferana (Clem.). The treatment rate was 20 Billion International Units of B. thuringiensis (B.t.) activity with or without 42 g of active ingredient of acephate/ha.The ground deposit of the standard Dipel wettable powder formulation was 12% of emitted volume compared with 21–32% for the Dipel 36B flowable. The viability of B.t. spores was drastically reduced after 1 day of weathering but a high level of biological activity by the spore–crystal complex persisted for up to 20 days post-spray due probably to crystal activity.The addition of about 10% of the recommended operational rate of acephate to the B.t. suspension increased larval mortality by 34% when applied at 4.7 l./ha. Reductions in budworm populations were 97–99% in B.t. + acephate plots and 86–90% in B.t. alone plots.Plots with moderate budworm densities of up to 27 larvae/100 buds on white spruce and 36/100 on balsam fir were satisfactorily protected from excessive defoliation in the year of spray by B.t. with or without acephate. Plots with higher population densities were not satisfactorily protected based on the branch sample examination but aerial color photographs indicated good protection to the top third of the trees. Population declines were greater and defoliation and oviposition were lower in the treated plots than in the untreated checks 1 year later without further treatment. Two years later the larval population densities in all plots were low but the density was twice as high in the untreated check as in the treated plots, indicating long term suppression by the treatments. Defoliation was negligible in all plots.The treatments had no deleterious effect on spruce budworm parasitism. The data indicate that the integrated approach using Bacillus thuringiensis – chemical pesticide combinations is a viable alternative to the use of chemical pesticides alone in spruce budworm control. Large scale testing is now warranted.

scholarly journals Efecto insecticida del extracto de semillas de Neem (Azadirachta indica) sobre Collaria scenica, Stal (Hemiptera: Miridae)

2012 ◽  
Vol 5 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Daniel Antonio Villamil Montero ◽  
Natalia Naranjo ◽  
Mario Andres Van Strahlen
Keyword(s):  
Thin Layer ◽  
Azadirachta Indica ◽  
Seed Extract ◽  
Neem Seed ◽  
Insecticidal Effect ◽  
Negative Effect ◽  
Number Of Individuals ◽  
Layer Chromatography ◽  
Mortality Percentage ◽  
Neem Seed Extract

El presente trabajo se desarrollo con el objetivo de evaluar o efeito insecticida del extracto etanólico de semillas de Neem (Azadirachta indica A. Juss) sobre ninfas de la chinche de los pastos Collaria scenica Stal. Para eso, se elaboró un extracto a partir de frutos inmaduros de Neem mediante rotaevaporación. El extracto fue diluido en tres concentraciones de ppm que corresponden a los tratamientos. Por medio de cromatografías en capa delgada se determinó la presencia de Azaridactina. Se realizó un experimento DCA de 4 tratamientos y 5 repeticiones que incluyó las tres concentraciones del extracto y un control. En cada repeticion se usaron 15 ninfas, colocadas aleatoriamente en cajas plásticas herméticas con alimento y la concentración correspondiente. Diariamente se realizó una aplicación del extracto y se registró porcentaje de mortalidad, número de exuvia y número de individuos que llegaron al estado adulto en cada tratamiento. Los resultados fueron analizados con prueba Kruskal-wallis y Games-Howell para cada variable. Las tres concentraciones del extracto de semillas de Neem presentaron un efecto negativo sobre el desarrollo de las chinches. El tratamiento más concentrado (250ppm) fue el más eficaz presentando una mortalidad del 97%, menor número de exubias y menor número adultos al final del ensayo. Insecticide Effect of Neem (Azadirachta indica A. Juss) Seed Extract against Collaria scenica Stal (Hemiptera: Miridae) Abstract. This work was developed with the objetive to evaluate the insecticidal effect of the Neem (Azadirachta indica A. Juss) seeds against grass bug nymphs Collaria scenica Stal. For that, an extract was prepared from green fruits of Neem by rotaevaporación. The extract was diluted in three ppm concentrations corresponding to treatments. Using thin-layer chromatography we identified the presence of Azaridactina. DCA essay was carried out with 4 treatments and 5 repetitions that included the three extract concentrations and a control. In each repetition were used 15 nymphs, randomly placed in plastic boxes with food and the corresponding concentration. Every day an extract application was made and recorded mortality percentage, number of exuviae and number of individuals who came to adulthood in each treatment. The results were analyzed using Kruskal-Wallis and Games-Howell for each variable. The three concentrations of Neem seed extract had a negative effect on the development of the bugs. The more concentrated treatment (250 ppm) was the most effective, showing a 97% mortality, fewer exuvias and and fewer adults in the end of the experiment.

EFFECT OF TEMPERATURE ON THE PATHOGENESIS OF BACILLUS THURINGIENSIS BERLINER IN LARVAE OF THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA CLEM. (LEPIDOPTERA: TORTRICIDAE)

1994 ◽  
Vol 126 (4) ◽  
pp. 1061-1065 ◽  
Author(s):  
Kees van Frankenhuyzen
Keyword(s):  
Bacillus Thuringiensis ◽  
Bacterial Growth ◽  
Choristoneura Fumiferana ◽  
Larval Mortality ◽  
Lethal Dose ◽  
Spruce Budworm ◽  
Effect Of Temperature ◽  
Vegetative Cells ◽  
Time Required ◽  
Peak Abundance

AbstractThe relationship between temperature and pathogenesis of Bacillus thuringiensis Berliner var. kurstaki in infected larvae of the eastern spruce budworm, Choristoneura fumiferana Clem., was investigated to determine if more rapid death of larvae with an increase in temperature could be accounted for by enhanced bacterial growth. Cumulative mortality of larvae force-fed with a lethal dose of HD-1-S-1980 peaked within 2 days at 25 °C, 3 days at 19 °C, 7 days at 16 °C, and 21 days at 13 °C. The progress of bacterial growth in the larvae was followed from spore germination to cell lysis, and was completed within 4 days at 25 °C, 6 days at 22 °C, 12 days at 19 °C, 14 days at 16 °C, and > 28 days at 13 °C. Peak abundance of vegetative cells in the larvae was observed after 1 day at 25 °C, 2 days at 22 °C, 3 days at 19 °C, 7 days at 16 °C, and 21 days at 13 °C, and thus coincided almost exactly with the time required for maximum larval mortality. This correlation suggests that the observed effect of temperature on progression of larval mortality was due to its effect on the proliferation of vegetative cells in the infected larvae, and that bacterial septicemia makes an important contribution to death.

scholarly journals Comparative role of neem seed extract, moringa leaf extract and imidacloprid in the management of wheat aphids in relation to yield losses in Pakistan

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0184639 ◽  
Author(s):  
Farhan Mahmood Shah ◽  
Muhammad Razaq ◽  
Abid Ali ◽  
Peng Han ◽  
Julian Chen
Keyword(s):  
Leaf Extract ◽  
Seed Extract ◽  
Neem Seed ◽  
Yield Losses ◽  
Neem Seed Extract ◽  
Moringa Leaf Extract

A METHOD FOR MASS PRODUCING SPORES OF THE MICROSPORIDIAN NOSEMA FUMIFERANAE IN ITS HOST, THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)

1976 ◽  
Vol 108 (4) ◽  
pp. 383-386 ◽  
Author(s):  
G. G. Wilson
Keyword(s):  
Mass Production ◽  
Choristoneura Fumiferana ◽  
Larval Mortality ◽  
Spruce Budworm ◽  
Fourth Instar ◽  
Spore Concentration ◽  
Spore Yield

AbstractThe mass production of Nosema fumiferanae spores using second, third, and fourth instar larvae of Choristoneura fumiferana is described. In general, second instar larvae inoculated with 2 × 105 spores per millilitre of suspension resulted in a maximum spore yield of 1.3 × 108 spores per larva. A spore concentration of 2 × 107 reduced larval weights and increased larval mortality.

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