PERSISTENCE OF NEODIPRION SERTIFER (HYMENOPTERA: DIPRIONIDAE) NUCLEAR POLYHEDROSIS VIRUS ON PINUS CONTORTA FOLIAGE

1983 ◽  
Vol 115 (7) ◽  
pp. 869-873 ◽  
Author(s):  
W. J. Kaupp
Keyword(s):  
Pinus Contorta ◽  
Nuclear Polyhedrosis Virus ◽  
Neodiprion Sertifer ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

AbstractThe persistence of Neodiprion sertifer nuclear polyhedrosis virus (NPV) on Pinus contorta foliage samples collected from sawfly infested plantations located throughout Britain was studied. Sawfly density and disease epizootiology varied between localities. Results from the bioassay of branch samples, divided into bark, needle base and foliage samples, indicated that N. sertifer NPV produced during a single virus epizootic can persist for at least 2 years on the pine foliage. Bark and needle base samples were found to be contaminated with NPV more often than foliage samples, perhaps reflecting the effect of weathering on the persistence of virus on the needle surface.

PERSISTENCE AND DISPERSAL OF THE NUCLEAR POLYHEDROSIS VIRUS OF NEODIPRION SERTIFER (GEOFFROY) (HYMENOPTERA: DIPRIONIDAE) IN A VIRUS-FREE LODGEPOLE PINE PLANTATION IN SWEDEN

1988 ◽  
Vol 120 (10) ◽  
pp. 887-892 ◽  
Author(s):  
Einar Olofsson
Keyword(s):  
Nuclear Polyhedrosis Virus ◽  
Lodgepole Pine ◽  
Larval Mortality ◽  
Neodiprion Sertifer ◽  
Experimental Plot ◽  
Pine Plantation ◽  
Larval Population ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
High Level

AbstractAn outbreak of Neodiprion sertifer (Geoffroy) was studied in a lodgepole pine plantation. It was the first tree generation on a 60-ha peatland area. The nuclear polyhedrosis virus (NPV) of N. sertifer was not found in the larval population or in the soil. Within a 1.7-ha experimental plot, a 0.35-ha block was treated with NPV and the ensuing epizootic was studied during three successive summers. The treatment caused 50% mortality of fourth- and fifth-instar larvae. The NPV persisted in the treated block and gradually dispersed into the adjacent blocks. After 2 years, larval mortality was 78% in the treated block and 21% at a distance of 110–125 m from it. The larval population remained at a high level and the outbreak expanded from the experimental plot to the entire 60-ha area in the years following the virus treatment, but few virus-diseased colonies were observed outside the experimental plot. Thus, the capability of this NPV to persist and spread was not sufficient to control and contain the sawfly outbreak.

scholarly journals Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 704
Author(s):  
Qian Yu ◽  
Pengfei Chang ◽  
Xiaoxuan Liu ◽  
Peng Lü ◽  
Qi Tang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bombyx Mori ◽  
Nuclear Polyhedrosis Virus ◽  
Expression System ◽  
Efficient Production ◽  
Human Bocavirus ◽  
Adeno Associated Virus ◽  
Silkworm Pupae ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

Recombinant adeno-associated virus (AAV) vectors have broad application prospects in the field of gene therapy. The establishment of low-cost and large-scale manufacturing is now the general agenda for industry. The baculovirus-insect cell/larva expression system has great potential for these applications due to its scalability and predictable biosafety. To establish a more efficient production system, Bombyx mori pupae were used as a new platform and infected with recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV). The production of a chimeric recombinant adeno-associated virus (rAAV) serotype 2/human bocavirus type-1 (HBoV1) vector was used to evaluate the efficiency of this new baculovirus expression vector (BEV)–insect expression system. For this purpose, we constructed two recombinant BmNPVs, which were named rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP. The yields of rAAV2/HBoV1 derived from the rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP co-infected BmN cells exceeded 2 × 104 vector genomes (VG) per cell. The rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP can express stably for at least five passages. Significantly, rAAV2/HBoV1 could be efficiently generated from BmNPV-infected silkworm larvae and pupae at average yields of 2.52 × 1012 VG/larva and 4.6 × 1012 VG/pupa, respectively. However, the vectors produced from the larvae and pupae had a high percentage of empty particles, which suggests that further optimization is required for this platform in the future. Our work shows that silkworm pupae, as an efficient bioreactor, have great potential for application in the production of gene therapy vectors.

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