BACILLUS THURINGIENSIS FOR CONTROLLING THREE SPECIES OF MOTHS IN STORED GRAIN

1976 ◽  
Vol 108 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Wm. H. McGaughey
Keyword(s):  
Bacillus Thuringiensis ◽  
Trichoplusia Ni ◽  
Aqueous Suspension ◽  
Stored Grain ◽  
Complete Control ◽  
Angoumois Grain Moth ◽  
Cadra Cautella ◽  
Wettable Powder ◽  
Indian Meal ◽  
Almond Moth

AbstractIn laboratory studies, an aqueous suspension of a commercial wettable powder formulation of Bacillus thuringiensis var. kurstaki (strain HD-1) containing at least 25 billion viable spores/g and 16000 International Units of Potency (Trichoplusia ni (Hübner))/mg prevented infestations of Indian meal moths, Plodia interpunctella (Hübner), and almond moths, Cadra cautella (Walker), in corn and wheat at a dose of ca. 120 mg of formulation/kg of grain. Treatment of a 100-mm-deep surface layer was more effective than treatments 33 or 67 mm deep and as effective as treatment of the entire grain mass Lack of uniformity in applying B. thuringiensis to the grain kernels caused a small decrease in effectiveness. Dust and bait formulations at about the same doses were as effective as the suspension, but the bait was undesirable because it supported the development of beetle larvae. The formulation was less effective in controlling the Angoumois grain moth, Sitotroga cerealella (Olivier): doses that gave complete control of the Indian meal moth and the almond moth reduced emergence of adult Angoumois grain moths by only about one-third.

Courtship behavior of phycitid moths. II. Behavioral and pheromonal isolation of Plodia interpunctella and Cadra cautella in the laboratory

1975 ◽  
Vol 53 (6) ◽  
pp. 827-832 ◽  
Author(s):  
G. G. Grant ◽  
E. B. Smithwick ◽  
U. E. Brady
Keyword(s):  
Sex Pheromone ◽  
Courtship Behavior ◽  
Species Recognition ◽  
Plodia Interpunctella ◽  
Indian Meal Moth ◽  
Cadra Cautella ◽  
Indian Meal ◽  
Almond Moth ◽  
Interspecific Courtship ◽  
Isolation Mechanisms

Interspecific courtship was observed between the Indian meal moth, Plodia interpunctella, and the almond moth, Cadra cautella. Successful cross-mating did not occur, however, because of the following isolation mechanisms: (1) incompatible courtship behavior, (2) male sex pheromone as a species recognition signal, (3) inhibition of males to female sex pheromone stimulation, and (4) mechanical or physiological barriers to insemination.Male Indian meal moths were strongly excited by calling almond moth females and courted them vigorously. However, because the courtship behavior of this combination was incompatible, copulation did not occur. Occasionally courtship continued to a later stage in the sequence but then the female rejected the male apparently because he releases an inappropriate aphrodisiac from his scent scales.In the reciprocal cross, fully two-thirds of the almond moth males failed to be sufficiently excited by Indian meal moth females to court them. Most of these males were inhibited, as indicated by their subsequent lack of response to pheromone extracts from their own female. Those males which were excited by Indian meal moth females courted them successfully and copulated, but insemination apparently did not occur. In a few cases the females rejected the males, as in the reverse cross.

Ovipositional Preference and Larval Establishment of the Indian Meal Moth and Almond Moth on Selected Peanut Genotypes1

1990 ◽  
Vol 17 (1) ◽  
pp. 12-15 ◽  
Author(s):  
R. K. Kashyap ◽  
W. V. Campbell
Keyword(s):  
Additional Data ◽  
Germplasm Collection ◽  
Indian Meal Moth ◽  
Cadra Cautella ◽  
Ovipositional Preference ◽  
Arachis Hypogaea L ◽  
Indian Meal ◽  
Almond Moth ◽  
Ovipositional Response ◽  
Larval Establishment

Abstract Thirty-nine selected peanut (Arachis hypogaea L.) genotypes from a diverse germplasm collection with known resistance and susceptibility to preharvest insects were tested in the laboratory for postharvest resistance to the Indian meal moth, Plodia interpunctella (Hubner), and the almond moth Cadra cautella (Walker). After the initial test the number of genotypes was reduced to 20 to reduce duplication of pedigree and omit some of the genotypes with intermediate resistance. Additional data were collected from the 20 genotypes on ovipositional preference and larval establishment on shelled and unshelled (farmers stock) peanuts. Genotypes with resistance to moth species included NC 6, GP-NC 343 × NC 5, PI 467307, NC 7 × NC 10247, GP-NC 343 × NC 17367 and breeding line 10-P10-B1-B1-B1-B1-B2. Resistance to preharvest and postharvest insect complexes suggest some common chemicals may be present in the green crop and the harvested seed that elicit ovipositional response.

scholarly journals Bacillus thuringiensis Bel Protein Enhances the Toxicity of Cry1Ac Protein to Helicoverpa armigera Larvae by Degrading Insect Intestinal Mucin

2009 ◽  
Vol 75 (16) ◽  
pp. 5237-5243 ◽  
Author(s):  
Shangling Fang ◽  
Li Wang ◽  
Wei Guo ◽  
Xia Zhang ◽  
Donghai Peng ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Armigera ◽  
Trichoplusia Ni ◽  
Knockout Mutant ◽  
Gene Encoding ◽  
In Vitro Degradation ◽  
Intestinal Mucin ◽  
Helicoverpa Armígera ◽  
Cry1ac Protein

ABSTRACT Bacillus thuringiensis has been used as a bioinsecticide to control agricultural insects. Bacillus cereus group genomes were found to have a Bacillus enhancin-like (bel) gene, encoding a peptide with 20 to 30% identity to viral enhancin protein, which can enhance viral infection by degradation of the peritrophic matrix (PM) of the insect midgut. In this study, the bel gene was found to have an activity similar to that of the viral enhancin gene. A bel knockout mutant was constructed by using a plasmid-free B. thuringiensis derivative, BMB171. The 50% lethal concentrations of this mutant plus the cry1Ac insecticidal protein gene were about 5.8-fold higher than those of the BMB171 strain. When purified Bel was mixed with the Cry1Ac protein and fed to Helicoverpa armigera larvae, 3 μg/ml Cry1Ac alone induced 34.2% mortality. Meanwhile, the mortality rate rose to 74.4% when the same amount of Cry1Ac was mixed with 0.8 μg/ml of Bel. Microscopic observation showed a significant disruption detected on the midgut PM of H. armigera larvae after they were fed Bel. In vitro degradation assays showed that Bel digested the intestinal mucin (IIM) of Trichoplusia ni and H. armigera larvae to various degrading products, similar to findings for viral enhancin. These results imply Bel toxicity enhancement depends on the destruction of midgut PM and IIM, similar to the case with viral enhancin. This discovery showed that Bel has the potential to enhance insecticidal activity of B. thuringiensis-based biopesticides and transgenic crops.

Efficacy of Cyfluthrin, Cyfluthrin + Piperonyl Butoxide, and Cyfluthrin + Piperonyl Butoxide + Chlorpyrifos-Methyl as Protectants of Stored Peanuts1

1994 ◽  
Vol 21 (1) ◽  
pp. 44-48 ◽  
Author(s):  
Frank H. Arthur
Keyword(s):  
Tribolium Castaneum ◽  
Plodia Interpunctella ◽  
Piperonyl Butoxide ◽  
Red Flour Beetle ◽  
Indianmeal Moth ◽  
Flour Beetle ◽  
Cadra Cautella ◽  
Almond Moth

Abstract Inshell peanuts were treated with 0.5, 1.0, 2.0, and 4.0 ppm cyfluthrin, each rate of cyfluthrin + 8.0 ppm piperonyl butoxide, and each rate of cyfluthrin + piperonyl butoxide + 25 ppm chlorpyrifos-methyl. After 10 months red flour beetle, Tribolium castaneum (Herbst), populations in peanuts treated with 0.5 and 1.0 ppm cyfluthrin averaged 89.5 and 34.2 adults per 12.7 kg peanuts; populations in peanuts treated with 1.0 and 1.5 ppm cyfluthrin + piperonyl butoxide averaged 72.0 amd 41.5 adults per 12.7 kg peanuts. Populations in the remaining 8 treatments ranged from 0.5 to 7.2 adults. Indianmeal moth, Plodia interpunctella (Hübner), and almond moth, Cadra cautella (Walker), populations remained low in all treatments. At 10 months the percentage of insectdamaged kernels from cracked pods ranged from 8.7 to 28.8% in the cyfluthrin and cyfluthrin + piperonyl butoxide treatments, while the percentage of damaged kernels was 4.4 to 6.1% in the 4 treatments with chlorpyrifos-methyl.

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