THE DIGESTIVE PROCESSES OF HAEMATOPHAGOUS INSECTS: IV. SECRETION OF TRYPSIN BY AEDES AEGYPTI (DIPTERA: CULICIDAE)

1973 ◽  
Vol 105 (4) ◽  
pp. 599-603 ◽  
Author(s):  
R. H. Gooding
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Aedes Aegypti ◽  
Blood Meal ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Trypsin Activity ◽  
Chymotrypsin Activity ◽  
Digestive Processes

AbstractThere is a correlation between the amount of blood ingested by Aedes aegypti (L.) and the trypsin activity (but not the chymotrypsin activity) in the midgut 16 and 24 hr after feeding. Puromycin and Dactinomycin ingested with a blood meal depress the level of midgut trypsin. Trypsin purified from the midguts of A. aegypti fed upon defibrinated blood containing 14C-amino acids contains radioactivity. Midguts from blood-fed mosquitoes did not produce significant amounts of trypsin when dissected from mosquitoes and maintained in a tissue culture medium for up to 50 hr.

EVIDENCE FOR THE INDUCTION OF URIDINE AND DEOXY-URIDINE PHOSPHORYLASES OF REGENERATING RAT LIVER IN VITRO

1965 ◽  
Vol 43 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Esther W. Yamada
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Rat Liver ◽  
Culture Medium ◽  
Specific Activity ◽  
Tissue Culture Medium ◽  
Uridine Phosphorylase ◽  
Regenerating Rat Liver ◽  
Specific Activities

Increases in the specific activities of undine and deoxyuridine phosphorylases of slices of regenerating rat liver were found 4 hours after incubation in tissue-culture medium containing uridine or 6-azauridine. These increases were not found when the tissue-culture medium contained either 8-azaguanine or puromycin, or when it lacked amino acids. Although both uridine and 6-azauridine were more effective in increasing the specific activity of uridine phosphorylase than that of deoxyuridine phosphorylase, azauridine was more effective than uridine in increasing the specific activities of both enzymes.In time studies, in which slices of regenerating rat liver were incubated in tissue-culture medium containing optimal concentrations of uridine, the specific activities of the two enzymes reached maximum levels at 3–4 hours. Puromycin prevented these increases.

Cultivation of Trypanosoma brucei sspp. in semi-defined and defined media

Parasitology ◽  
1973 ◽  
Vol 67 (3) ◽  
pp. 315-331 ◽  
Author(s):  
G. A. M. Cross ◽  
J. C. Manning
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Trypanosoma Brucei ◽  
Culture Medium ◽  
Defined Medium ◽  
Tissue Culture Medium ◽  
Optimum Temperature ◽  
Acid Hydrolysate ◽  
Defined Media

Semi-defined and defined media for the growth of culture forms of Trypanosoma brucei sspp. have been developed by enrichment of tissue culture medium 199 with additional vitamins, amino acids, salts and other compounds. The semi-defined medium contains an acid hydrolysate of casein: in the empirically devised defined medium the casein requirement has been circumvented by inclusion of additional vitamins and amino acids. Both media are very hypertonic. Control of pH was found to be particularly critical for growth. The optimum temperature for growth in the semidefined medium was between 25°C and 28°C, but cells would undergo one or two division cycles at 37°C.

ON TETHELIN AS A TISSUE CULTURE MEDIUM

1930 ◽  
Vol 1 (9) ◽  
pp. 289-290
Author(s):  
K. C. Richardson ◽  
E. S. Horning
Keyword(s):  
Tissue Culture ◽  
Culture Medium ◽  
Tissue Culture Medium

scholarly journals THE ANTIGENIC POTENCY OF EPIDEMIC INFLUENZA VIRUS FOLLOWING INACTIVATION BY ULTRAVIOLET RADIATION

1940 ◽  
Vol 72 (6) ◽  
pp. 729-745 ◽  
Author(s):  
Jonas E. Salk ◽  
G. I. Lavin ◽  
Thomas Francis
Keyword(s):  
Tissue Culture ◽  
Influenza Virus ◽  
Ultraviolet Radiation ◽  
Culture Medium ◽  
Tissue Culture Medium ◽  
Intraperitoneal Route ◽  
Active Virus ◽  
High Concentrations

A study of the antigenic potency of influenza virus inactivated by ultraviolet radiation has been made. Virus so inactivated is still capable of functioning as an immunizing agent when given to mice by the intraperitoneal route. In high concentrations inactivated virus appears to be nearly as effective as active virus but when quantitative comparisons of the immunity induced by different dilutions are made, it is seen that a hundredfold loss in immunizing capacity occurs during inactivation. Virus in suspensions prepared from the lungs of infected mice is inactivated more rapidly than virus in tissue culture medium. A standard for the comparison of vaccines of epidemic influenza virus is proposed.

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