FEEDING BEHAVIOUR OF THE PALES WEEVIL: II. RESPONSE TO SOME NEUTRAL LIPID FRACTIONS OF LOBLOLLY PINE PHLOEM

1972 ◽  
Vol 104 (3) ◽  
pp. 427-432
Author(s):  
H. A. Thomas
Keyword(s):  
Neutral Lipid ◽  
Loblolly Pine ◽  
Feeding Behaviour ◽  
Neutral Lipids ◽  
Feeding Stimulant ◽  
Lipid Fractions ◽  
Hylobius Pales

AbstractThe lipids of loblolly pine phloem, particularly the neutral lipids, were fractionated and bioassayed for feeding stimulant activity with the pales weevil, Hylobius pales (Herbst). Activity was evidently reduced by the fractionation procedures. Significant feeding occurred in response to the fraction whose principal constituent was β-sitosterol. Also, evidence indicated that substances occurring with the sterols may play a part in inducing feeding.

Pairing and aggregation of Amblosoma suwaense (Trematoda: Brachylaimidae) metacercariae in vitro and partial characterization of lipids involved in chemo-attraction

Parasitology ◽  
1981 ◽  
Vol 82 (2) ◽  
pp. 225-229 ◽  
Author(s):  
B. Fried ◽  
G. A. Robinson
Keyword(s):  
Thin Layer ◽  
Neutral Lipid ◽  
Sterol Ester ◽  
Neutral Lipids ◽  
Sterol Esters ◽  
Free Sterols ◽  
Lipid Fractions ◽  
Oil Red O

SUMMARYHistochemical and thin-layer chromatographic (t.l.c.) analyses were made on neutral lipids in the free (unencysted) metacercariae of Amblosoma suwaense (Brachylaimidae). As determined by t.l.c. the major neutral lipid fractions in metacercariae removed directly from Campeloma decisum snails were free sterols and sterol esters. Metacercariae incubated for 1 h at 37±1° C in sterile Locke's solution released mainly sterol esters and a lesser amount of free sterols into the medium. As determined by Oil Red O (ORO) staining, metacercariae accumulated neutral lipid in the intestinal caeca during incubation and the excretory system was ORO negative. Behavioural studies showed that metacereariae paired and aggregated in vitro and were attracted to lipophilic but not to hydrophilic worm products. Following t.l.c. preparative analysis it was demonstrated that metacercariae were attracted to sterol ester worm products but not to free sterol products.

scholarly journals Impact of Antiandrogen Treatment on the Fatty Acid Profile of Neutral Lipids in Human Meibomian Gland Secretions1

2000 ◽  
Vol 85 (12) ◽  
pp. 4866-4873 ◽  
Author(s):  
Benjamin D. Sullivan ◽  
James E. Evans ◽  
Kathleen L. Krenzer ◽  
M. Reza Dana ◽  
David A. Sullivan
Keyword(s):  
Fatty Acid ◽  
Neutral Lipid ◽  
High Performance ◽  
Neutral Lipids ◽  
Correlation Coefficients ◽  
Meibomian Gland ◽  
Age Related ◽  
Lipid Fractions ◽  
Antiandrogen Therapy ◽  
Very High

The purpose of this study was to determine whether the use of antiandrogen medications is associated with significant alterations in the fatty acid (FA) profiles of neutral lipids in human meibomian gland secretions. Meibomian gland secretions were obtained from both eyes of patients receiving antiandrogen therapy and from age-related controls. Samples were processed for high-performance liquid chromatography/mass spectrometry and an evaluation of the mass/charge ratios of neutral lipid FA. Our results demonstrate that antiandrogen therapy is associated with significant and consistent alterations in the mass/charge ratios of neutral lipid fractions of meibomian gland secretions. Patients taking antiandrogen medications had significant changes in the occurrence of numerous diglyceride, triglyceride, and wax/cholesterol ester FA products, compared with age-matched controls. Statistical analyses of data within groups demonstrated very high correlation coefficients, and cross-correlation analyses revealed characteristic shifts in FA patterns between groups. Our findings show that antiandrogen use is paralleled by significant changes in the FA profiles of neutral lipid fractions in meibomian gland secretions.

FEEDING BEHAVIOUR OF THE PALES WEEVIL, HYLOBIUS PALES (COLEOPTERA: CURCULIONIDAE): I. SYNERGISTIC EFFECTS WITH LOBLOLLY PINE PHLOEM EXTRACTS

1971 ◽  
Vol 103 (1) ◽  
pp. 74-79 ◽  
Author(s):  
H. A. Thomas ◽  
J. D. White
Keyword(s):  
Synergistic Effect ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Feeding Behaviour ◽  
Synergistic Effects ◽  
Lipid Fraction ◽  
Neutral Lipid Fraction ◽  
Polar Lipid Fraction ◽  
Hylobius Pales ◽  
Intensity Of Feeding

AbstractA bioassay with elder pith wafers was developed to measure sustained feeding of the pales weevil, Hylobius pales (Herbst). Sucrose, fructose, glucose, and extracts of phloem of loblolly pine (Pinus taeda L.) were compared in three-choice experiments. Intensity of feeding indicated a synergistic effect occurred when sucrose and phloem lipid were present together. The non-polar or neutral lipid fraction of phloem stimulated more feeding than did the polar lipid fraction.

scholarly journals Fatty Acids Profiles of Stipe and Blade from the Norwegian Brown Macroalga Laminaria hyperborea, Using Off-Line SPE and GC-MS

2016 ◽  
Author(s):  
Lena Oksdøl Foseid ◽  
Hanne Devle ◽  
Yngve Stenstrøm ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Lipid Fraction ◽  
Environmental Benefits ◽  
Laminaria Hyperborea ◽  
Alpha Linolenic Acid ◽  
Lipid Fractions

A thorough analysis and comparison of the fatty acid profiles of stipe and blade from Laminaria hyperborea, a kelp species found in the northern Atlantic, is presented. Lipids were extracted and fractionated into neutral lipids, free fatty acids and polar lipids, then derivatized to fatty acid methyl esters prior to GC-MS analysis. A total of 42 fatty acids were identified and quantified, including the n-3 fatty acids α-linolenic acid, stearidonic acid and eicosapentaenoic acid. An n-6/n-3 ratio of 0.8:1 was found in blade and 3.5:1 in stipe, respectively. The ratios vary between the lipid fractions within stipe and blade, with the lowest ratio in the polar lipid fraction of blade. The fatty acid amounts are higher in blade than in stipe, and the highest amounts of n-3 fatty acids are found within the neutral lipid fractions. The amounts of polyunsaturated fatty acids are 3.4 times higher in blade than stipe. This study highlights the compositional differences between the lipid fractions of stipe and blade from L. hyperborea. The amount of polyunsaturated fatty acids, compared to saturated- and monounsaturated fatty acids, as well as the n-6/n-3-ratio, is known to influence human health. In the pharmaceutical, food, and feed industries this can be of importance for production and sale of different health products. Additionally, lipids are today among the unused by products of alginate production, exploiting this material for commercial interest should give both economical and environmental benefits.

scholarly journals An essential vesicular-trafficking phospholipase mediates neutral lipid synthesis and contributes to hemozoin formation in Plasmodium falciparum

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Mohd Asad ◽  
Yoshiki Yamaryo-Botté ◽  
Mohammad E. Hossain ◽  
Vandana Thakur ◽  
Shaifali Jain ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Neutral Lipid ◽  
Neutral Lipids ◽  
Lipid Synthesis ◽  
Large Family ◽  
Degradation Pathway ◽  
Food Vacuole ◽  
Parasite Growth ◽  
Lipid Homeostasis ◽  
Parasite Development

Abstract Background Plasmodium falciparum is the pathogen responsible for the most devastating form of human malaria. As it replicates asexually in the erythrocytes of its human host, the parasite feeds on haemoglobin uptaken from these cells. Heme, a toxic by-product of haemoglobin utilization by the parasite, is neutralized into inert hemozoin in the food vacuole of the parasite. Lipid homeostasis and phospholipid metabolism are crucial for this process, as well as for the parasite’s survival and propagation within the host. P. falciparum harbours a uniquely large family of phospholipases, which are suggested to play key roles in lipid metabolism and utilization. Results Here, we show that one of the parasite phospholipase (P. falciparum lysophospholipase, PfLPL1) plays an essential role in lipid homeostasis linked with the haemoglobin degradation and heme conversion pathway. Fluorescence tagging showed that the PfLPL1 in infected blood cells localizes to dynamic vesicular structures that traffic from the host-parasite interface at the parasite periphery, through the cytosol, to get incorporated into a large vesicular lipid rich body next to the food-vacuole. PfLPL1 is shown to harbour enzymatic activity to catabolize phospholipids, and its transient downregulation in the parasite caused a significant reduction of neutral lipids in the food vacuole-associated lipid bodies. This hindered the conversion of heme, originating from host haemoglobin, into the hemozoin, and disrupted the parasite development cycle and parasite growth. Detailed lipidomic analyses of inducible knock-down parasites deciphered the functional role of PfLPL1 in generation of neutral lipid through recycling of phospholipids. Further, exogenous fatty-acids were able to complement downregulation of PfLPL1 to rescue the parasite growth as well as restore hemozoin levels. Conclusions We found that the transient downregulation of PfLPL1 in the parasite disrupted lipid homeostasis and caused a reduction in neutral lipids essentially required for heme to hemozoin conversion. Our study suggests a crucial link between phospholipid catabolism and generation of neutral lipids (TAGs) with the host haemoglobin degradation pathway.

scholarly journals The genetics of neutral lipid biosynthesis: an evolutionary perspective

2009 ◽  
Vol 297 (1) ◽  
pp. E19-E27 ◽  
Author(s):  
Aaron R. Turkish ◽  
Stephen L. Sturley
Keyword(s):  
Neutral Lipid ◽  
Metabolic Pathways ◽  
Fatty Liver Disease ◽  
Molecular Mechanisms ◽  
Neutral Lipids ◽  
Lipid Synthesis ◽  
Therapeutic Interventions ◽  
Wax Ester ◽  
Evolutionary Perspective ◽  
Nonalcoholic Fatty Liver

The storage of fatty acids and fatty alcohols in the form of neutral lipids such as triacylglycerol (TAG), cholesteryl ester (CE), and wax ester (WE) serves to provide reservoirs for membrane formation and maintenance, lipoprotein trafficking, lipid detoxification, evaporation barriers, and fuel in times of stress or nutrient deprivation. This ancient process likely originated in actinomycetes and has persisted in eukaryotes, albeit by different molecular mechanisms. A surfeit of neutral lipids is strongly, perhaps causally, related to several human diseases such as diabetes mellitus, obesity, atherosclerosis and nonalcoholic fatty liver disease. Therefore, understanding the metabolic pathways of neutral lipid synthesis and the roles of the enzymes involved may facilitate the development of new therapeutic interventions for these syndromes.

Lipid metabolism in Achlya: changes in lipid composition during development

1976 ◽  
Vol 22 (12) ◽  
pp. 1716-1719 ◽  
Author(s):  
Simon W. T. Law ◽  
David N. Burton
Keyword(s):  
Fatty Acids ◽  
Life Cycle ◽  
Fatty Acid ◽  
Neutral Lipid ◽  
Total Lipid ◽  
Eicosatetraenoic Acid ◽  
Developmental Cycle ◽  
Lipid Fractions ◽  
Low Levels

Fractionation of total lipid extracted from Achlya sp. at various stages of its developmental cycle revealed that in spores total lipid was composed of 62% neutral lipid, 13% phospholipid, and 25% glycolipid. After germination, the proportion of neutral lipid rose slightly after 2 h then fell sharply to 10% after 8 h, whereupon it rose to 55% of total lipid after 30 h of growth, when sporulation was completed. Conversely, phospholipid rose to 77% of total lipid after 8 h, then declined to 40% after 30 h. Glycolipid was maintained at 10–20% of total lipid throughout the life cycle after spore germination. Quantitative determination of neutral lipid components by photoreflectometry showed that triglycerides accounted for 20% of neutral lipid in spores, and free fatty acids made up 50%. During growth, the absolute levels of both components fell precipitously on germination, remained at low levels throughout vegetative growth, and rose at the time of sporulation.The fatty acid composition of total lipid, phospholipid, neutral lipid, and free fatty acid fractions extracted from vegetative and sporulating Achlya cells was determined. The principal fatty acids present in all fractions at both stages of the life cycle were hexadecanoic and octadecanoic acids. Hydroxyhexadecanoic acid, eicosatetraenoic acid, and an unidentified long-chain acid were completely absent from the phospholipids of vegetative cells, although they were found in significant quantities in lipid fractions from other stages of growth.

scholarly journals Influence of diets containing different natural oils on the incorporation of [1-14C]acetate in the various lipid fractions of rat liver

1966 ◽  
Vol 20 (3) ◽  
pp. 553-559 ◽  
Author(s):  
E. P. M. Bhattathiry
Keyword(s):  
Fatty Acids ◽  
Olive Oil ◽  
Palm Oil ◽  
Neutral Lipids ◽  
Total Lipids ◽  
Stock Diet ◽  
Natural Oils ◽  
Lipid Fractions ◽  
Normal Stock

1. A comparative study was undertaken with rats on the effect of various diets (normal stock, fat-free, palm oil and olive oil) on the in vitro incorporation of [14C]acetate by the liver into cholesterol and into the fatty acids of phospholipids and neutral fats. 2. The total lipids extracted from the incubation mixtures were fractionated into acetone-precipitable and digi- tonin-precipitable portions and also into the fatty acids of neutral lipids. 3. The incorporation of [14C]acetate into the acetone-precipitable fraction and into fatty acids of neutral fats was greatest in livers of rats given the fat-free diet, followed by those of the groups given olive oil, the normal stock diet, and palm oil. Livers from the group given the fat-free diet also exhibited the highest percentage of 14C activity in the digitonin-precipitable fraction and were closely followed by the group on the normal stock diet. Compared with those of the other two groups, the livers of the groups given olive oil and palm oil showed much less activity in the digitonin- precipitable fraction. 4. The greater the amount of a specific type of fatty acid in the diet, the less was the 14C activity incorporated into that type of fattyacid in the ncutral fats of liver slices, hut this was not so with the fatty acids obtained froin the acetone-precipitahlc fraction of the lipids.

scholarly journals The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets

2011 ◽  
Vol 192 (6) ◽  
pp. 1043-1055 ◽  
Author(s):  
Oludotun Adeyo ◽  
Patrick J. Horn ◽  
SungKyung Lee ◽  
Derk D. Binns ◽  
Anita Chandrahas ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Neutral Lipid ◽  
Strong Evidence ◽  
Lipid Droplets ◽  
Neutral Lipids ◽  
Glucose Medium ◽  
Wild Type ◽  
Lipid Levels ◽  
Total Neutral Lipid ◽  
A Site

Lipins are phosphatidate phosphatases that generate diacylglycerol (DAG). In this study, we report that yeast lipin, Pah1p, controls the formation of cytosolic lipid droplets. Disruption of PAH1 resulted in a 63% decrease in droplet number, although total neutral lipid levels did not change. This was accompanied by an accumulation of neutral lipids in the endoplasmic reticulum (ER). The droplet biogenesis defect was not a result of alterations in neutral lipid ratios. No droplets were visible in the absence of both PAH1 and steryl acyltransferases when grown in glucose medium, even though the strain produces as much triacylglycerol as wild type. The requirement of PAH1 for normal droplet formation can be bypassed by a knockout of DGK1. Nem1p, the activator of Pah1p, localizes to a single punctum per cell on the ER that is usually next to a droplet, suggesting that it is a site of droplet assembly. Overall, this study provides strong evidence that DAG generated by Pah1p is important for droplet biogenesis.

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