Method of extraction influences the detected short chain fatty acid concentration in human fecal samples

SciVee ◽  
2008 ◽  
Keyword(s):  
Fatty Acid ◽  
Acid Concentration ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Fatty Acid Concentration ◽  
Fecal Samples ◽  
Method Of Extraction

scholarly journals Effects of probiotics on loperamide-induced constipation in rats

2020 ◽  
Author(s):  
Takio Inatomi
Keyword(s):  
Body Weight ◽  
Fatty Acid ◽  
Oral Administration ◽  
Acid Concentration ◽  
Short Chain Fatty Acid ◽  
Phosphate Buffer Solution ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Fatty Acid Concentration ◽  
Sd Rats

AbstractConstipation, a common health problem, causes discomfort and affects quality of life. This study aimed to evaluate the potential effect of probiotics on loperamide (LP)-induced constipation in Sprague-Dawley (SD) rats, a well-established animal model of spastic constipation. In total, 100 male SD rats (age, 8 weeks; Japan SLC, Inc., Hamamatsu, Japan) were used in the experiments following a 12-day acclimatisation period. They were randomly divided into two treatment groups (groups 1 and 2) of 50 rats each. Spastic constipation was induced via oral administration of LP (3 mg/kg) for 6 days 1 hour before the administration of each test compound. Similarly, a probiotics solution (4 ml/kg body weight) was orally administered to the rats once a day for 6 days in group 2. In group 1, phosphate buffer solution was orally administered once a day for 6 days 1 hour after each LP administration. The changes in body weight, faecal parameters, short-chain fatty acid concentration in faeces, and faecal immunoglobulin (Ig)-A concentration were recorded. In the present study, the oral administration of probiotics improved faecal parameters, short-chain fatty acid concentration in faeces, and faecal IgA concentration. Our results indicate that probiotics increase the levels of intestinal short-chain fatty acids, especially butyric acid, thereby improving constipation and intestinal immunity.

A dietary sodium gluconate supplement improves growth performance and prebiotic activity in the small intestine of nursery pigs grown under tropical conditions

2011 ◽  
Vol 51 (8) ◽  
pp. 702 ◽  
Author(s):  
T. Poeikhampha ◽  
C. Bunchasak
Keyword(s):  
Fatty Acid ◽  
Growth Performance ◽  
Acid Concentration ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Intestinal Morphology ◽  
Short Chain ◽  
Fatty Acid Concentration ◽  
Sodium Gluconate ◽  
Nursery Pigs

This study was conducted to evaluate the effect of sodium gluconate (SG) supplementation in diets on growth performance, small intestinal morphology and short chain fatty acid concentration in the caecum of nursery pigs. One-hundred and forty-four piglets (bodyweight ~10.5 kg) were raised in conventional open housing and divided into four treatments with six replications. The pigs received diet supplemented without (control), or with SG at a level of 1000, 2500 or 5000 ppm for 6 weeks. The results indicated that increasing SG supplementation from 0 to 5000 ppm linearly improved final bodyweight, average daily gain, feed : gain ratio, caecal propionic acid and total short-chain fatty acid concentration (linear P < 0.05). Adding SG in diet tended to reduce Escherichia coli counts in the caecum (P = 0.09) and adding SG at 2500–5000 ppm significantly increased the villous height in the duodenum (linear, P < 0.01), whereas there were no increase in caecal acetic acid and lactic acid bacterial counts in the caecum and rectum, and E. coli counts in the rectum when compared with the control group. SG supplementation did not affect the pH values in gastrointestinal tract of nursery pigs. This study implied that SG supplementation improved growth rate and may be useful as a prebiotic to accomplish the maximum growth performances in nursery pigs.

scholarly journals Effect of Lactosucrose Feeding on Cecal pH, Short-Chain Fatty Acid Concentration and Microflora in Rats.

1992 ◽  
Vol 45 (2) ◽  
pp. 109-115 ◽  
Author(s):  
Masaru YONEYAMA ◽  
Takahiko MANDAI ◽  
Hajime AGA ◽  
Kazuko FUJII ◽  
Shuzo SAKAI ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Concentration ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Fatty Acid Concentration

scholarly journals 80 Effects of miscanthus fiber on nutrient digestibility and fecal metabolites of adult dogs

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 58-58
Author(s):  
Shannon E Finet ◽  
Fei He ◽  
Heather Mangian ◽  
Maria R C de Godoy
Keyword(s):  
Fatty Acid ◽  
Dietary Fiber ◽  
Acid Concentration ◽  
Resistant Starch ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Fatty Acid Concentration ◽  
Tomato Pomace ◽  
Beet Pulp

Abstract Dietary fiber is an important component of pet food that helps to promote colonocyte health by favoring saccharolytic fermentation and short-chain fatty acid production in addition to modulating gut microbiota and aiding laxation. The objective of this study was to evaluate and compare the effects of miscanthus grass fiber, prebiotic fiber blends, and traditional dietary fiber sources on apparent total tract digestibility, fecal metabolites, and gastrointestinal intolerance in adult canines. Animal procedures were approved by the University of Illinois Institutional Animal Care and Use Committee. Twelve female adult beagles were randomly assigned to one of six treatments containing cellulose, beet pulp, miscanthus fiber, or a blend of miscanthus fiber and tomato pomace, miscanthus fiber and resistant starch, or miscanthus fiber and fructooligosaccharide in a replicated 6x6 Latin square design. All treatment diets were formulated to meet or exceed AAFCO nutrient profile (2018). The dogs received each treatment for 21 d, with 17 d of diet adaptation followed by 4 d of total and fresh fecal collection. No difference in fecal score was observed (P &lt; 0.05). Dogs fed the beet pulp diet had the highest organic matter digestibility and total dietary fiber digestibility (P &lt; 0.05) when compared to all other treatments. Crude protein digestibility was significantly higher (P &lt; 0.05) for the cellulose, miscanthus fiber, and miscanthus and resistant starch blend than the beet pulp diet. Fecal total short-chain fatty acid concentration was greatest for beet pulp and lowest for the cellulose treatment (P &lt; 0.05) with the miscanthus fiber and miscanthus fiber blends with resistant starch, fructooligosaccharide, and tomato pomace being intermediate. No difference was observed in fecal total branched-chain fatty acid concentration (P &lt; 0.05). The data suggest that miscanthus fiber is an adequate and functional source of fiber in extruded canine diets, and comparable to the traditional fiber source, cellulose.

scholarly journals Analysis of fecal short chain fatty acid concentration in miniature dachshunds with inflammatory colorectal polyps

2017 ◽  
Vol 79 (10) ◽  
pp. 1727-1734 ◽  
Author(s):  
Hirotaka IGARASHI ◽  
Koichi OHNO ◽  
Naoaki MATSUKI ◽  
Aki FUJIWARA-IGARASHI ◽  
Hideyuki KANEMOTO ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Acid Concentration ◽  
Short Chain Fatty Acid ◽  
Colorectal Polyps ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Fatty Acid Concentration
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