3D Super-resolution Microscopy by Computational Optical Imaging

SciVee ◽  
2011 ◽  
Author(s):  
Rafael Piestun
Keyword(s):  
Optical Imaging ◽  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals All-Optical Imaging of Gold Nanoparticle Geometry Using Super-Resolution Microscopy

2018 ◽  
Vol 122 (4) ◽  
pp. 2336-2342 ◽  
Author(s):  
Adam Taylor ◽  
René Verhoef ◽  
Michael Beuwer ◽  
Yuyang Wang ◽  
Peter Zijlstra
Keyword(s):  
Optical Imaging ◽  
Gold Nanoparticle ◽  
Super Resolution ◽  
All Optical ◽  
Super Resolution Microscopy

scholarly journals The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Hieng Chiong Tie ◽  
Alexander Ludwig ◽  
Sara Sandin ◽  
Lei Lu
Keyword(s):  
Optical Imaging ◽  
Golgi Complex ◽  
Spatial Organization ◽  
Super Resolution ◽  
Spatial Separation ◽  
Dense Packing ◽  
Golgi Stack ◽  
Central Disk ◽  
En Face ◽  
Super Resolution Microscopy

It is unclear how the two principal functions of the Golgi complex, processing and transport, are spatially organized. Studying such spatial organization by optical imaging is challenging, partially due to the dense packing of stochastically oriented Golgi stacks. Using super-resolution microscopy and markers such as Giantin, we developed a method to identify en face and side views of individual nocodazole-induced Golgi mini-stacks. Our imaging uncovered that Golgi enzymes preferentially localize to the cisternal interior, appearing as a central disk or inner-ring, whereas components of the trafficking machinery reside at the periphery of the stack, including the cisternal rim. Interestingly, conventional secretory cargos appeared at the cisternal interior during their intra-Golgi trafficking and transiently localized to the cisternal rim before exiting the Golgi. In contrast, bulky cargos were found only at the rim. Our study therefore directly demonstrates the spatial separation of processing and transport functions within the Golgi complex.

scholarly journals High-Resolution Optical Imaging and Sensing Using Quantum Emitters in Hexagonal Boron-Nitride

2021 ◽  
Vol 9 ◽  
Author(s):  
Carlo Bradac
Keyword(s):  
High Resolution ◽  
Boron Nitride ◽  
Optical Imaging ◽  
Hexagonal Boron Nitride ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Sensing Applications ◽  
Resolution Imaging ◽  
Super Resolution Microscopy ◽  
Quantum Emitters

Super-resolution microscopy has allowed optical imaging to reach resolutions well beyond the limit imposed by the diffraction of light. The advancement of super-resolution techniques is often an application-driven endeavor. However, progress in material science plays a central role too, as it allows for the synthesis and engineering of nanomaterials with the unique chemical and physical properties required to realize super-resolution imaging strategies. This aspect is the focus of this review. We show that quantum emitters in two-dimensional hexagonal boron nitride are proving to be excellent candidate systems for the realization of advanced high-resolution imaging techniques, and spin-based quantum sensing applications.

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy

Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins

2019 ◽  
Author(s):  
Jeffrey Chang ◽  
Matthew Romei ◽  
Steven Boxer
Keyword(s):  
Rational Design ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Unit Cell Size ◽  
Chlorine Substituent ◽  
Gfp Chromophore ◽  
The One ◽  
Green Fluorescent ◽  
Super Resolution Microscopy

<p>Double-bond photoisomerization in molecules such as the green fluorescent protein (GFP) chromophore can occur either via a volume-demanding one-bond-flip pathway or via a volume-conserving hula-twist pathway. Understanding the factors that determine the pathway of photoisomerization would inform the rational design of photoswitchable GFPs as improved tools for super-resolution microscopy. In this communication, we reveal the photoisomerization pathway of a photoswitchable GFP, rsEGFP2, by solving crystal structures of <i>cis</i> and <i>trans</i> rsEGFP2 containing a monochlorinated chromophore. The position of the chlorine substituent in the <i>trans</i> state breaks the symmetry of the phenolate ring of the chromophore and allows us to distinguish the two pathways. Surprisingly, we find that the pathway depends on the arrangement of protein monomers within the crystal lattice: in a looser packing, the one-bond-flip occurs, whereas in a tighter packing (7% smaller unit cell size), the hula-twist occurs.</p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p> <p> </p>

Novel organic dyes for multicolor localization-based super-resolution microscopy

2015 ◽  
Vol 9 (1-2) ◽  
pp. 161-170 ◽  
Author(s):  
Martin Lehmann ◽  
Gregor Lichtner ◽  
Haider Klenz ◽  
Jan Schmoranzer
Keyword(s):  
Organic Dyes ◽  
Super Resolution ◽  
Super Resolution Microscopy
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