scholarly journals A CRISPR/Cas9 cleavage system for capturing fungal secondary metabolite gene clusters

2020 ◽  
Author(s):  
Xinran Xu ◽  
Jin Feng ◽  
Peng Zhang ◽  
Jie Fan ◽  
Wen-Bing Yin
Keyword(s):  
Secondary Metabolite ◽  
Gene Clusters ◽  
Fungal Secondary Metabolite

scholarly journals Microbial communication leading to the activation of silent fungal secondary metabolite gene clusters

2015 ◽  
Vol 6 ◽  
Author(s):  
Tina Netzker ◽  
Juliane Fischer ◽  
Jakob Weber ◽  
Derek J. Mattern ◽  
Claudia C. König ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Gene Clusters ◽  
Microbial Communication ◽  
Fungal Secondary Metabolite

Evolutionary Mechanisms Involved in Development of Fungal Secondary Metabolite Gene Clusters

2014 ◽  
pp. 341-356 ◽  
Author(s):  
Geromy G. Moore ◽  
Jérôme Collemare ◽  
Marc-Henri Lebrun ◽  
Rosie E. Bradshaw
Keyword(s):  
Secondary Metabolite ◽  
Gene Clusters ◽  
Evolutionary Mechanisms ◽  
Fungal Secondary Metabolite

Analyzing Fungal Secondary Metabolite Genes and Gene Clusters

2014 ◽  
pp. 171-193 ◽  
Author(s):  
Yi-Ming Chiang ◽  
Clay C.C. Wang ◽  
Berl R. Oakley
Keyword(s):  
Secondary Metabolite ◽  
Gene Clusters ◽  
Fungal Secondary Metabolite

Genome sequence of Novosphingobium malaysiense strain MUSC 273T, novel alpha-proteobacterium isolated from intertidal soil

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Hooi-Leng Ser ◽  
Wai-Fong Yin ◽  
Kok-Gan Chan ◽  
Nurul-Syakima Ab Mutalib ◽  
Learn-Han Lee
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Genome Sequence ◽  
Catalase Activity ◽  
Gene Clusters ◽  
Rrna Genes ◽  
Gram Negative ◽  
Genomic Features

Novosphingobium malaysiense strain MUSC 273T is a recently identified Gram-negative, aerobic alpha-proteobacterium. The strain was isolated from intertidal soil with strong catalase activity. The genome sequence comprises 5,027,021 bp, with 50 tRNA and 3 rRNA genes. Further analysis identified presence of secondary metabolite gene clusters within genome of MUSC 273T. Knowledge of the genomic features of the strain may allow further biotechnological exploitation, particularly for production of secondary metabolites as well as production of industrially important enzymes

Identification of the kinanthraquinone biosynthetic gene cluster by expression of an atypical response regulator

2021 ◽  
Vol 85 (3) ◽  
pp. 714-721
Author(s):  
Risa Takao ◽  
Katsuyuki Sakai ◽  
Hiroyuki Koshino ◽  
Hiroyuki Osada ◽  
Shunji Takahashi
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Response Regulator ◽  
Bac Library ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Gene Organization ◽  
Biosynthetic Gene ◽  
Streptomyces Sp

ABSTRACT Recent advances in genome sequencing have revealed a variety of secondary metabolite biosynthetic gene clusters in actinomycetes. Understanding the biosynthetic mechanism controlling secondary metabolite production is important for utilizing these gene clusters. In this study, we focused on the kinanthraquinone biosynthetic gene cluster, which has not been identified yet in Streptomyces sp. SN-593. Based on chemical structure, 5 type II polyketide synthase gene clusters were listed from the genome sequence of Streptomyces sp. SN-593. Among them, a candidate gene cluster was selected by comparing the gene organization with grincamycin, which is synthesized through an intermediate similar to kinanthraquinone. We initially utilized a BAC library for subcloning the kiq gene cluster, performed heterologous expression in Streptomyces lividans TK23, and identified the production of kinanthraquinone and kinanthraquinone B. We also found that heterologous expression of kiqA, which belongs to the DNA-binding response regulator OmpR family, dramatically enhanced the production of kinanthraquinones.

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