scholarly journals Increased antibiotic resistance of methicillin-resistance Staphylococcus aureus USA300 Δpsm mutants and a complementation study of Δpsm mutants using synthetic phenol soluble modulins

2020 ◽  
Author(s):  
Hun-Suk Song ◽  
Shashi Kant Bhatia ◽  
Tae-Rim Choi ◽  
Ranjit Gurav ◽  
Hyun Joong Kim ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Methicillin Resistance ◽  
Complementation Study

Investigation of the antibiotic resistance of staphylococcus species isolated from foods

2021 ◽  
Vol 67 (2) ◽  
pp. 3372-3382
Author(s):  
Brigitta Horváth ◽  
Ferenc Peles ◽  
Judit Gasparikné Reichardt ◽  
Edit Pocklán ◽  
Rita Sipos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Methicillin Resistance ◽  
Multidrug Resistant ◽  
Farm Animals ◽  
The European Union ◽  
Methicillin Resistant ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

The presence of methicillin-resistant Staphylococcus aureus (MRSA) strains in the food chain has been confirmed by several studies in the European Union, but there are only limited data available in Hungary. The objective of the present study was to investigate the antibiotic resistance of Staphylococcus strains isolated from foods, using classical microbiological, molecular biological methods and the MALDI-TOF-MS technique, as well as the multi-locus sequence typing (MLST) of antibiotic resistant strains. During the study, 47 coagulase-positive (CPS) and 30 coagulase-negative (CNS) Staphylococcus isolates were collected. In the course of the MALDI-TOF-MS investigations, all CPS isolates (n=47) were found to be S. aureus species, while 8 different species were identified in the case of the CNS strains. Methicillin resistance was confirmed in two S. aureus strains, one of which had a sequence type not yet known, while the other MRSA strain was type ST398, which is the most common type of MRSA strain isolated from farm animals in the EU/EEA. (The abbreviation “MRSA” is often used in common parlance, but occasionally in the literature to denote “multidrug-resistant Staphylococcus aureus”. In the authors’ manuscript - the methicillin-resistant pathogen is correctly designated as such. Ed.)

scholarly journals Carriage Methicillin-Resistant Staphylococcus aureus in Poultry and Cattle in Northern Algeria

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Saliha Bounar-Kechih ◽  
Mossadak Taha Hamdi ◽  
Hebib Aggad ◽  
Nacima Meguenni ◽  
Zafer Cantekin
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Broiler Chickens ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Laying Hens ◽  
Methicillin Resistance ◽  
Public Health Problem ◽  
Cross Resistance ◽  
Isolation And Identification ◽  
Methicillin Resistant

Multiresistant and especially Methicillin-Resistant Staphylococcus aureus (MRSA) poses a serious public health problem that requires their immediate identification and antibiotic resistance characteristics. In order to determine antibiotic resistance S. aureus poultry and bovine origin, 8840 samples were collected from slaughterhouses in the northern region of Algeria between years 2009 and 2014. 8375 samples were from an avian origin (1875 from laying hens and 6500 from broiler chickens) and the rest was from bovine origin. Bacteriological isolation and identification were made by classical culture method and antibiotic resistance patterns were determined by disc diffusion test. The prevalence of S. aureus was 42% in laying hens, 12% in broilers, and 55% in bovine samples. The prevalence of MRSA was 57%, 50%, and 31% in laying hens, broiler chickens, and bovine, respectively. While MRSA strains isolated from poultry showed cross-resistance to aminoglycosides, fluoroquinolones, macrolides, sulphonamides, and cyclins, those isolated from bovine also revealed similar multiresistance except for sulphonamide. This high percentage of methicillin resistance and multidrug resistance in S. aureus poultry and bovine origin may have importance for human health and curing of human infections.

Large-scale mass spectrometry data combined with demographics analysis rapidly predicts methicillin resistance in Staphylococcus aureus

2020 ◽  
Author(s):  
Zhuo Wang ◽  
Hsin-Yao Wang ◽  
Chia-Ru Chung ◽  
Jorng-Tzong Horng ◽  
Jang-Jih Lu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Large Scale ◽  
Operating Characteristic ◽  
Susceptibility Testing ◽  
Methicillin Resistance ◽  
Characteristic Curve ◽  
Mass Spectrometry Data ◽  
Treatment Efficiency

Abstract Background A mass spectrometry-based assessment of methicillin resistance in Staphylococcus aureus would have huge potential in addressing fast and effective prediction of antibiotic resistance. Since delays in the traditional antibiotic susceptibility testing, methicillin-resistant S. aureus remains a serious threat to human health. Results Here, linking a 7 years of longitudinal study from two cohorts in the Taiwan area of over 20 000 individually resolved methicillin susceptibility testing results, we identify associations of methicillin resistance with the demographics and mass spectrometry data. When combined together, these connections allow for machine-learning-based predictions of methicillin resistance, with an area under the receiver operating characteristic curve of >0.85 in both the discovery [95% confidence interval (CI) 0.88–0.90] and replication (95% CI 0.84–0.86) populations. Conclusions Our predictive model facilitates early detection for methicillin resistance of patients with S. aureus infection. The large-scale antibiotic resistance study has unbiasedly highlighted putative candidates that could improve trials of treatment efficiency and inform on prescriptions.

scholarly journals Prevalence of methicillin resistance and superantigenic toxins in Staphylococcus aureus strains isolated from patients with cancer

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Effat Abbasi Montazeri ◽  
Azar Dokht Khosravi ◽  
Saeedeh Khazaei ◽  
Ali Sabbagh
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Cancer Patients ◽  
Methicillin Resistance ◽  
Resistance Pattern ◽  
Patients With Cancer ◽  
Resistance Patterns ◽  
Mrsa Strains ◽  
Southwest Iran ◽  
Antibiotic Resistance Patterns

Abstract Background This study aimed to determine the frequency of methicillin-resistant Staphylococcus aureus (MRSA), antibiotic resistance patterns, superantigenic toxins profile, and clonality of this pathogen in patients with cancer. Results In total, 79 (25.7%) isolates were confirmed as Staphylococcus species, from which 38 (48.1%) isolates were S. aureus, and 29 (76.3%) isolates were confirmed as MRSA. The highest resistance in MRSA strains was seen against ciprofloxacin (86.2%) and erythromycin (82.8%). Teicoplanin, and linezolid were the most effective antibiotics. From all MRSA isolates, 3 strains (10.3%) were resistant to vancomycin with minimum inhibitory concentration values of 128 μg/ml. The prevalence of superantigenic toxins genes was as follows: pvl (10.5%), tsst-1 (36.8%), etA (23.7%), and etB (23.7%). The t14870 spa type with frequency of 39.5% was the most prevalent clone type circulating in the cancer patients. Conclusions This study showed the circulating of spa t14870 as the most predominant MRSA clone in cancer patients of southwest Iran. Also, a diverse antibiotic resistance pattern and toxin profiles were seen among MRSA isolates.

Molecular Investigation of Staphylococcal Cassette Chromosome mec (SCCmec) Elements Isolated from Intensive Care Unit

2020 ◽  
Author(s):  
Fahimeh Nourbakhsh ◽  
Vajiheh Nourbakhsh ◽  
Samaneh Borooni ◽  
Elaheh Tajbakhsh ◽  
Dana Daneshmand
Keyword(s):  
Intensive Care Unit ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Intensive Care ◽  
Methicillin Resistance ◽  
Diffusion Method ◽  
Clinical Samples ◽  
High Morbidity ◽  
Molecular Investigation ◽  
Mrsa Strains

Background and Aims: Based on the results, Staphylococcus aureus is one of the serious infectious agents found in community and hospitals with remarkable potential for high morbidity and mortality around the globe. The present study was carried out for molecular investigation of methicillinresistant Staphylococcus aureus strains and Staphylococcal Chromosomal Cassette mec (SCCmec) phenotypes isolated from the intensive care unit in Hazrat Fatemeh Zahra hospital of Isfahan. Materials and Methods: A total of 76 clinical wound samples were collected from Hazrat Fatemeh Zahra Hospital in Isfahan and evaluated by polymerase chain reaction (PCR) methods. The Methicillin resistance Staphylococcus aureus (MRSA) screening was performed by genotypic and phenotypic methods; also antibiotic resistance pattern was determined by using the disk diffusion method and related genes by PCR. Results: Totally, 53 (69.7%) out of 76 clinical samples were positive for MRSA. Of the 76 MRSA strains, 39 (63.51%) were PVL positive (51.3%). The most commonly infected samples were collected from wounds (40.8%). The most commonly detected antibiotic resistance genes were mecA (89.61%), tetK (88.23%), tetM (49.15%) and msrA (46.93%). Resultantly, it was shown that MRSA has the highest level of resistance against methicillin (98%), penicillin (97.24%), tetracycline (89.64%). It was also revealed that the most commonly detected SCCmec types in the MRSA strains are types II (14.53%) and III (16.82%). Conclusions: In summary, this paper argues that the orderly surveillance of hospital-associated infections and initial management and supervision of the antibiotic resistance patterns are required to control the prevalence of MRSA.

scholarly journals Prevalence of Staphylococcus aureus carriage and pattern of antibiotic resistance, including methicillin resistance, among contact sport athletes in Italy

2019 ◽  
Vol Volume 12 ◽  
pp. 1161-1170 ◽  
Author(s):  
Valentina Mascaro ◽  
Maria Simona Capano ◽  
Teresa Iona ◽  
Carmelo Giuseppe Angelo Nobile ◽  
Antonio Ammendolia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Methicillin Resistance ◽  
Contact Sport

scholarly journals An overview of Staphylococcus epidermidis and Staphylococcus aureus with a focus on developing countries

2015 ◽  
Vol 9 (06) ◽  
pp. 547-550 ◽  
Author(s):  
Daniela Chessa ◽  
Giulia Ganau ◽  
Vittorio Mazzarello
Keyword(s):  
Staphylococcus Aureus ◽  
Developing Countries ◽  
Antibiotic Resistance ◽  
Life Cycle ◽  
Virulence Factors ◽  
Staphylococcus Epidermidis ◽  
Methicillin Resistance ◽  
The World ◽  
Spa Gene ◽  
And Staphylococcus Aureus

Most nosocomial infections by Staphylococcus epidermidis and Staphylococcus aureus have gained considerable attention due to an increase of infections caused by these strains that have been reported in recent years throughout the world. Most notably, it is important to underline the presence of S. epidermidis and S. aureus in the human epithelia microflora and to highlight that it is impossible to eradicate them from humans. There are various virulence factors that normally sustain the infection life cycle, such as antibiotic resistance (methicillin resistance). Furthermore, it is important to evaluate the usefulness of typing the spa gene from isolated strains in order to study genotypes and geographical distributions. In the present review, different cases related to patients infected by Staphylococci and an overview of this problem worldwide are reported.

Cloning and Nucleotide Sequence Determination of the Entire mec DNA of Pre-Methicillin-Resistant Staphylococcus aureus N315

1999 ◽  
Vol 43 (6) ◽  
pp. 1449-1458 ◽  
Author(s):  
T. Ito ◽  
Y. Katayama ◽  
K. Hiramatsu
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Methicillin Resistance ◽  
Integration Site ◽  
Bacterial Species ◽  
Antibiotic Resistance Genes ◽  
Direct Repeat ◽  
Methicillin Resistant ◽  
Reading Frame

ABSTRACT In methicillin-resistant Staphylococcus aureus, the methicillin resistance gene mecA is localized within a large chromosomal region which is absent in the methicillin-susceptibleS. aureus chromosome. The region, designatedmec DNA, is speculated to have originated from the genome of another bacterial species and become integrated into the chromosome of the S. aureus cell in the past. We report here cloning and determination of the structure of the entire mec DNA sequence from a Japanese S. aureus strain, N315. Themec DNA was found to be 51,669 bp long, including terminal inverted repeats of 27 bp and a characteristic pair of direct repeat sequences of 15 bp each: one is situated in the right extremity ofmec DNA, and the other is situated outside themec DNA and abuts the left boundary of mec DNA. The integration site of mec DNA was found to be located in an open reading frame (ORF) of unknown function, designatedorfX. Clusters of antibiotic resistance genes were noted inmec DNA carried by transposon Tn554 and an integrated copy of plasmid pUB110. Both the transposon and plasmid were integrated in the proximity of the mecA gene, the latter being flanked by a pair of insertion sequence IS431elements. Many ORFs other than those encoding antibiotic resistance were considered nonfunctional because of the acquired mutations or partial deletions found in the ORFs. Two ORFs potentially encoding novel site-specific recombinases were found in mec DNA. However, there was no ORF that might encode mecDNA-specific transposase or integrase proteins, indicating that themec DNA is not a transposon or a bacteriophage in nature.

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