scholarly journals Development of the Mammalian Expression Vector System that can be Induced by IPTG and/or Lactose

2020 ◽  
Vol 30 (8) ◽  
pp. 1124-1131 ◽  
Author(s):  
Seung-Hyun Myung ◽  
Junghee Park ◽  
Ji-Hye Han ◽  
Tae-Hyoung kim
Keyword(s):  
Expression Vector ◽  
Vector System ◽  
Mammalian Expression ◽  
Mammalian Expression Vector

High-level synthesis of biologically active reovirus protein σ1in a mammalian expression vector system

Virology ◽  
1988 ◽  
Vol 167 (2) ◽  
pp. 601-612
Author(s):  
A BANERJEA ◽  
K BRECHLING ◽  
C RAY ◽  
H ERIKSON ◽  
D PICKUP ◽  
...  
Keyword(s):  
Expression Vector ◽  
Biologically Active ◽  
High Level Synthesis ◽  
Vector System ◽  
Mammalian Expression ◽  
Mammalian Expression Vector ◽  
High Level

High-level synthesis of biologically active reovirus protein σ1 in a mammalian expression vector system

Virology ◽  
1988 ◽  
Vol 167 (2) ◽  
pp. 601-612 ◽  
Author(s):  
Akhil C. Banerjea ◽  
Kathleen A. Brechling ◽  
Caroline A. Ray ◽  
Harold Erikson ◽  
David J. Pickup ◽  
...  
Keyword(s):  
Expression Vector ◽  
Biologically Active ◽  
High Level Synthesis ◽  
Vector System ◽  
Mammalian Expression ◽  
Mammalian Expression Vector ◽  
High Level

scholarly journals Characterization of LL25X, a Newly Isolated Anti-SIV Monoclonal Antibody, to Determine Binding Specificity

Elements ◽  
2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Zackary Tajin Park
Keyword(s):  
Phage Display ◽  
Mammalian Cells ◽  
Expression Vector ◽  
Restriction Enzymes ◽  
Phage Display Library ◽  
Single Chain ◽  
Phage Display Technology ◽  
Mammalian Expression ◽  
Mammalian Expression Vector ◽  
Single Chain Fv

A phage display library was previously constructed from an SIV-infected rhesus macaque. Several single chain Fv (scFv), including SU24, SU343 and LL25X, were selected using phage display technology. Sequences corresponding to SU24, SU343 and LL25X were optimized for expression in a mammalian system and commercially synthesized. SU24 and SU343 had previously been cloned into a mammalian expression vector. In this study, we aimed to characterize the specificity of SU24, SU343, and LL25X.. The codon-optimized version of the scFv LL25X gene sequence was cloned into a mammalian expression vector (pCEP4).  LL25X DNA was amplified by PCR, and the PCR product and mammalian expression vector were both digested with KpnI/SapI restriction enzymes. Digested fragments were purified, and the fragments were ligated using T4DNA ligase. E. coli cells were transformed with the ligation reaction. Single colonies were selected on LB agar plates containing the selective antibiotic (ampicillin). Positive colonies were identified after DNA mini-preparation and test-digestion with KpnI and SapI. Sanger sequencing confirmed cloning results and DNA sequence accuracy. Following transfection of mammalian cells (293T), LL25X-Fc cells, and purifying our protein, the binding of LL25X-Fc to the SIV gp140 envelope protein was confirmed via ELISA and Western Blotting.

scholarly journals Imaging grafted cells with [18F]FHBG using an optimized HSV1-TK mammalian expression vector in a brain injury rodent model

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0184630 ◽  
Author(s):  
Anne-Sophie Salabert ◽  
Laurence Vaysse ◽  
Marie Beaurain ◽  
Mathieu Alonso ◽  
Germain Arribarat ◽  
...  
Keyword(s):  
Brain Injury ◽  
Expression Vector ◽  
Rodent Model ◽  
Mammalian Expression ◽  
Mammalian Expression Vector

Plasmid pLTRpoly: a versatile high-efficiency mammalian expression vector

Gene ◽  
1992 ◽  
Vol 118 (2) ◽  
pp. 293-294 ◽  
Author(s):  
Tomi P. Mäkelä ◽  
Juha Partanen ◽  
Manfred Schwab ◽  
Kari Alitalo
Keyword(s):  
Expression Vector ◽  
High Efficiency ◽  
Mammalian Expression ◽  
Mammalian Expression Vector

scholarly journals Efficient Production of HIV-1 Virus-Like Particles from a Mammalian Expression Vector Requires the N-Terminal Capsid Domain

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e28314 ◽  
Author(s):  
Pascal Jalaguier ◽  
Karine Turcotte ◽  
Alexis Danylo ◽  
Réjean Cantin ◽  
Michel J. Tremblay
Keyword(s):  
Expression Vector ◽  
Efficient Production ◽  
Virus Like Particles ◽  
Mammalian Expression ◽  
Mammalian Expression Vector ◽  
Hiv 1
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