Antibiofilm and Anti-��-Lactamase Activities of Burdock Root Extract and Chlorogenic Acid against Klebsiella pneumoniae

2017 ◽  
Vol 27 (3) ◽  
pp. 542-551 ◽  
Author(s):  
Satish Kumar Rajasekharan ◽  
Samiraj Ramesh ◽  
Ann Susan Satish ◽  
Jintae Lee
Keyword(s):  
Klebsiella Pneumoniae ◽  
Chlorogenic Acid ◽  
Root Extract

scholarly journals Smilax aristolochiifolia Root Extract and Its Compounds Chlorogenic Acid and Astilbin Inhibit the Activity of α-Amylase and α-Glucosidase Enzymes

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Viridiana Candelaria Pérez-Nájera ◽  
Janet Alejandra Gutiérrez-Uribe ◽  
Marilena Antunes-Ricardo ◽  
Sergio Hidalgo-Figueroa ◽  
Carmen Lizette Del-Toro-Sánchez ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Metabolic Effects ◽  
Root Extract ◽  
Enzymatic Assays ◽  
Glucosidase Activity ◽  
Hydrolyzing Enzymes ◽  
Hydroethanolic Extract ◽  
Fast Centrifugal Partition Chromatography ◽  
Main Strategy

Regulating activities of α-amylase and α-glucosidase through the use of specific inhibitors is a main strategy for controlling type 2 diabetes. Smilax aristolochiifolia root decoctions are traditionally used in Mexico as hypoglycemic and for weight loss, but the active principles and mechanisms underlying such putative metabolic effects are yet unknown. Here, we isolated the major bioactive compounds from a hydroethanolic extract of S. aristolochiifolia root by fast centrifugal partition chromatography and evaluated their effects against pancreatic α-amylase and yeast α-glucosidase. A chlorogenic acid-rich fraction (CAF) inhibited α-amylase activity with an IC50 value of 59.28 μg/mL in an uncompetitive manner and α-glucosidase activity with an IC50 value of 9.27 μg/mL in a noncompetitive mode. Also, an astilbin-rich fraction (ABF) inhibited α-glucosidase activity with an IC50 value of 12.30 μg/mL, in a noncompetitive manner. CAF inhibition α-amylase was as active as acarbose while both CAF and ABF were 50-fold more potent inhibitors of α-glucosidase than acarbose. The molecular docking results of chlorogenic acid and astilbin with α-amylase and α-glucosidase enzymes correlated with the inhibition mechanisms suggested by enzymatic assays. Our results prove that S. aristolochiifolia roots contain chlorogenic acid and astilbin, which inhibit carbohydrates-hydrolyzing enzymes, suggesting a new mechanism for the hypoglycemic effect reported for this plant.

Chlorogenic acid ameliorates Klebsiella pneumoniae-induced pneumonia in immunosuppressed mice via inhibiting the activation of NLRP3 inflammasomes

2021 ◽  
Author(s):  
Junhao Zeng ◽  
Xiaoyu Wan ◽  
Ting Liu ◽  
Ying Xiong ◽  
Gan Xiang ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Chlorogenic Acid ◽  
Bioactive Properties ◽  
Nlrp3 Inflammasomes ◽  
Anti Inflammation ◽  
Immunosuppressed Mice

Chlorogenic acid (CGA) possesses a wide variety of bioactive properties, such as antioxidation, anti-inflammation and anti-bacteria. This study was aimed at exploring the effects of CGA of anti-inflammation and anti-bacteria...

ANTI-BACTERIAL ACTIVITY OF ROOT EXTRACT OF ACONITUM HETEROPHYLLUM AGAINST SOME PATHOGENIC BACTERIA

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (09) ◽  
pp. 36-39
Author(s):  
K Kalaichelvi ◽  
◽  
S. Menaga
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Maximum Activity ◽  
Bacterial Activity ◽  
Root Extract ◽  
Aconitum Heterophyllum ◽  
Ethanol Extracts ◽  
Aqueous Root Extract

Aqueous and ethanol extracts of Aconitum heterophyllum on five test bacteria such as (Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescences and Staphylococcus faecalis) exhibited concentration dependent antibacterial activity. All fractions showed a promising activity towards Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescences and Staphylococcus faecalis, however less inhibition was observed in Bacillus cereus. Similarly when compared to aqueous root extract, ethanol root extract showed maximum activity against all the tested organisms.

scholarly journals Using Phytochemicals to Investigate the Activation of Nicotine Detoxification via Upregulation of CYP2A6 in Animal Models Exposed Tobacco Smoke Condensate by Intratracheal Instillation

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Dayeon Lee ◽  
Seung-Beom Seo ◽  
Hyun Jeong Lee ◽  
Tae-Sik Park ◽  
Soon-Mi Shim
Keyword(s):  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Tobacco Smoke ◽  
A549 Cells ◽  
Intratracheal Instillation ◽  
Root Extract ◽  
Ionization Mass ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Tobacco Smoke Condensate

This study examined the efficacy of standardized Smilax china L. root extract (SSCR) containing chlorogenic acid on detoxifying nicotine from tobacco smoke condensate (TSC) in vitro and in vivo. Chlorogenic acid is an identified bioactive component in SSCR by ultraperformance liquid chromatography/photodiode array/electrospray ionization/mass spectroscopy (UPLC/PDA/ESI/MS). HepG2 liver cells and A549 lung cells were carried for measuring ROS and antioxidant enzymes. Sprague-Dawley rats were treated with nicotine by intratracheal instillation (ITI). Cell viabilities by pretreatments of 5, 12.5, and 25, 50 μg SSCR/mL ranged from 41 to 76% in HepG2 and 65 to 95% in A549. Pretreatments of SSCR inhibited TSC-mediated production of reactive oxygen species (ROS) by 8 and 10% in HepG2 and A549 cells, respectively. However, the expression of CAT, SOD1, and AOX1 was downregulated by SSCR in the both cells. The highest conversion of cotinine was observed at 50 μg/mL of SSCR after 120 min of incubation. SSCR upregulated CYP2A6 3-fold in A549 cells regardless of TSC cotreatment. When Sprague-Dawley rats were treated with nicotine by ITI or subjected to SSCR administration for 14 days, the levels of cotinine in urine increased in SSCR treatment only. The cellular level of antioxidant capacity at 10 or 100 mg/kg body weight/day of SSCR treatment was 1.89 and 1.86 times higher than those of nicotine-control. Results suggest that the intake of SSCR can detoxify nicotine by elevating nicotine conversion to cotinine and antioxidant capacity.

Tobacco Mosaic Virus-Infected Tissue

1985 ◽  
Vol 43 ◽  
pp. 510-511
Author(s):  
Egbert W. Henry
Keyword(s):  
Nicotiana Tabacum ◽  
Tobacco Mosaic Virus ◽  
Chlorogenic Acid ◽  
Mosaic Virus ◽  
Host Resistance ◽  
Oxidative Metabolism ◽  
Leaf Tissue ◽  
Vein Clearing ◽  
Basal Septum ◽  
Concentration Levels

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.

Triterpenoid saponins from the cytotoxic root extract of Sideroxylon foetidissimum, an endemic Yucatecan medicinal plant

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
A Sánchez-Medina ◽  
PC Stevenson ◽  
S Habtemariam ◽  
LM Peña-Rodríguez ◽  
O Corcoran ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Root Extract ◽  
Triterpenoid Saponins

Protective activities of the aqueous root extract of Harungana madagascariensis in acute and repeated acetaminophen hepatotoxic rats

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
AA Adeneye ◽  
JA Olagunju ◽  
SO Elias ◽  
OD Olatunbosun ◽  
AO Mustafa ◽  
...  
Keyword(s):  
Root Extract ◽  
Aqueous Root Extract
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