Insecticidal Activity and Histopathological Effects of Vip3Aa Protein from Bacillus thuringiensis on Spodoptera litura

2016 ◽  
Vol 26 (10) ◽  
pp. 1774-1780 ◽  
Author(s):  
Feifei Song ◽  
Yunfeng Lin ◽  
Chen Chen ◽  
Ensi Shao ◽  
Xiong Guan ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Spodoptera Litura ◽  
Histopathological Effects

scholarly journals Oligomer Formation and Insecticidal Activity of Bacillus thuringiensis Vip3Aa Toxin

Toxins ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 274 ◽  
Author(s):  
Ensi Shao ◽  
Aishan Zhang ◽  
Yaqi Yan ◽  
Yaomin Wang ◽  
Xinyi Jia ◽  
...  
Keyword(s):  
Amino Acid ◽  
Bacillus Thuringiensis ◽  
Complex Formation ◽  
Insecticidal Activity ◽  
Spodoptera Litura ◽  
Insecticidal Proteins ◽  
Proteolytic Activation ◽  
Oligomer Formation ◽  
Lepidopteran Insects ◽  
Substitution Mutations

Bacillus thuringiensis (Bt) Vip3A proteins are important insecticidal proteins used for control of lepidopteran insects. However, the mode of action of Vip3A toxin is still unclear. In this study, the amino acid residue S164 in Vip3Aa was identified to be critical for the toxicity in Spodoptera litura. Results from substitution mutations of the S164 indicate that the insecticidal activity of Vip3Aa correlated with the formation of a >240 kDa complex of the toxin upon proteolytic activation. The >240 kDa complex was found to be composed of the 19 kDa and the 65 kDa fragments of Vip3Aa. Substitution of the S164 in Vip3Aa protein with Ala or Pro resulted in loss of the >240 kDa complex and loss of toxicity in Spodoptera litura. In contrast, substitution of S164 with Thr did not affect the >240 kDa complex formation, and the toxicity of the mutant was only reduced by 35%. Therefore, the results from this study indicated that formation of the >240 kDa complex correlates with the toxicity of Vip3Aa in insects and the residue S164 is important for the formation of the complex.

Engineered Bacillus thuringiensis GO33A with broad insecticidal activity against lepidopteran and coleopteran pests

2006 ◽  
Vol 72 (5) ◽  
pp. 924-930 ◽  
Author(s):  
Guangjun Wang ◽  
Jie Zhang ◽  
Fuping Song ◽  
Jun Wu ◽  
Shuliang Feng ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity

Dual Insecticidal Activity of Spodoptera-Toxic Bacillus thuringiensis Strain Transformed with Lepidopteran-Specific Cry Toxin

2007 ◽  
Vol 10 (2) ◽  
pp. 137-143
Author(s):  
Joong Nam Kang ◽  
Jong Yul Roh ◽  
Sang Chul Shin ◽  
Sang-Hyun Koh ◽  
Yeong Jin Chung ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Cry Toxin ◽  
Thuringiensis Strain
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