Enhancement of Herboxidiene Production in Streptomyces chromofuscus ATCC 49982

2014 ◽  
Vol 24 (1) ◽  
pp. 52-58 ◽  
Author(s):  
Amit Kumar Jha ◽  
Janardan Lamichhane ◽  
Jae Kyung Sohng
Keyword(s):  
Streptomyces Chromofuscus

scholarly journals Transphosphatidylation capacity of phospholipase D from cabbage (Brassica oleracea L. var. capitata L.) leaves and Streptomyces chromofuscus

1999 ◽  
Vol 25 (5) ◽  
pp. 229-237
Author(s):  
Toshihiro WATANABE ◽  
Hiroaki SATO ◽  
Yozo NAKAZAWA ◽  
Yoshimasa SAGANE ◽  
T. Tsuneo KOZIMA ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Phospholipase D ◽  
Streptomyces Chromofuscus ◽  
Brassica Oleracea L

scholarly journals Symmetrically substituted dichlorophenes inhibit N-acyl-phosphatidylethanolamine phospholipase D

2020 ◽  
Vol 295 (21) ◽  
pp. 7289-7300 ◽  
Author(s):  
Geetika Aggarwal ◽  
Jonah E. Zarrow ◽  
Zahra Mashhadi ◽  
C. Robb Flynn ◽  
Paige Vinson ◽  
...  
Keyword(s):  
Bile Acids ◽  
Phospholipase D ◽  
Cultured Cells ◽  
Lithocholic Acid ◽  
Hek293 Cells ◽  
Serum Lipase ◽  
Structure Activity ◽  
Molecule Inhibitor ◽  
Streptomyces Chromofuscus

N-Acyl-phosphatidylethanolamine phospholipase D (NAPE-PLD) (EC 3.1.4.4) catalyzes the final step in the biosynthesis of N-acyl-ethanolamides. Reduced NAPE-PLD expression and activity may contribute to obesity and inflammation, but a lack of effective NAPE-PLD inhibitors has been a major obstacle to elucidating the role of NAPE-PLD and N-acyl-ethanolamide biosynthesis in these processes. The endogenous bile acid lithocholic acid (LCA) inhibits NAPE-PLD activity (with an IC50 of 68 μm), but LCA is also a highly potent ligand for TGR5 (EC50 0.52 μm). Recently, the first selective small-molecule inhibitor of NAPE-PLD, ARN19874, has been reported (having an IC50 of 34 μm). To identify more potent inhibitors of NAPE-PLD, here we used a quenched fluorescent NAPE analog, PED-A1, as a substrate for recombinant mouse Nape-pld to screen a panel of bile acids and a library of experimental compounds (the Spectrum Collection). Muricholic acids and several other bile acids inhibited Nape-pld with potency similar to that of LCA. We identified 14 potent Nape-pld inhibitors in the Spectrum Collection, with the two most potent (IC50 = ∼2 μm) being symmetrically substituted dichlorophenes, i.e. hexachlorophene and bithionol. Structure–activity relationship assays using additional substituted dichlorophenes identified key moieties needed for Nape-pld inhibition. Both hexachlorophene and bithionol exhibited significant selectivity for Nape-pld compared with nontarget lipase activities such as Streptomyces chromofuscus PLD or serum lipase. Both also effectively inhibited NAPE-PLD activity in cultured HEK293 cells. We conclude that symmetrically substituted dichlorophenes potently inhibit NAPE-PLD in cultured cells and have significant selectivity for NAPE-PLD versus other tissue-associated lipases.

Enhanced degradation of ammonium-pretreated wheat straw by lignocellulolytic Streptomyces spp.

1992 ◽  
Vol 38 (10) ◽  
pp. 1022-1025 ◽  
Author(s):  
Marina Basaglia ◽  
Giuseppe Concheri ◽  
Stefano Cardinali ◽  
Maria B. Pasti-Grigsby ◽  
Marco P. Nuti
Keyword(s):  
Weight Loss ◽  
Wheat Straw ◽  
Lignin Degradation ◽  
Cellulose Degradation ◽  
Lignocellulose Degradation ◽  
Cellulose Content ◽  
Streptomyces Spp ◽  
Initial Substrate ◽  
Streptomyces Chromofuscus ◽  
Pretreated Wheat Straw

Eleven actinomycetes, isolated from the gut of worker termites (Macrotermes, Armitermes, Microcerotermes, Odontotermes), were identified as Streptomyces chromofuscus, S. chromogenus, S. diastaticus, and S. rochei. Their ability to grow on natural lignocellulosic substrates was tested in solid state fermentation experiments using wheat straw (C/N = 49.8) as a sole carbon source. Weight loss was 4.7–20.9% of the initial substrate, after 5 weeks at 30 °C; lignin and cellulose content decreased 2.0–16.1 and 3.5–32.9%, respectively. When the 11 Streptomyces were grown on wheat straw pretreated with (NH4)HCO3 (C/N = 28.2), weight loss was 9.3–29.9% of the initial substrate, indicating an overall enhancement of lignocellulose degradation. Weight, lignin, and cellulose losses were enhanced when S. chromofuscus (strain A2 and A11) and S. rochei A4 were grown on pretreated wheat straw instead of the untreated substrate. With S. rochei A10 the weight loss and lignin degradation were enhanced, while cellulolysis was slightly depressed. Weight loss and cellulose degradation were both enhanced when the remaining strains were grown on pretreated wheat straw. In this case, lignin degradation was depressed (S. chromofuscus A6 and A8, S. diastaticus A12, S. rochei A14) or remained essentially the same (S. diastaticus A3 and S. chromogenus A7). Key words: Streptomyces, wheat straw, degradation, lignin, cellulose.

scholarly journals Herboxidiene, a new herbicidal substance from Streptomyces chromofuscus A7847. Taxonomy, fermentation, isolation, physico-chemical and biological properties.

1992 ◽  
Vol 45 (6) ◽  
pp. 914-921 ◽  
Author(s):  
MARGARET MILLER-WIDEMAN ◽  
NARINDER MAKKAR ◽  
MINHTIEN TRAN ◽  
BARBARA ISAAC ◽  
NANCY BIEST ◽  
...  
Keyword(s):  
Biological Properties ◽  
Physico Chemical ◽  
Streptomyces Chromofuscus

scholarly journals Transphosphatidylation activity of Streptomyces chromofuscus phospholipase D in biomimetic membranes

2003 ◽  
Vol 270 (22) ◽  
pp. 4523-4530 ◽  
Author(s):  
Karim El Kirat ◽  
Annie-France Prigent ◽  
Jean-Paul Chauvet ◽  
Bernard Roux ◽  
Francoise Besson
Keyword(s):  
Phospholipase D ◽  
Biomimetic Membranes ◽  
Streptomyces Chromofuscus

scholarly journals Identification of the Herboxidiene Biosynthetic Gene Cluster in Streptomyces chromofuscus ATCC 49982

2012 ◽  
Vol 78 (6) ◽  
pp. 2034-2038 ◽  
Author(s):  
Lei Shao ◽  
Jiachen Zi ◽  
Jia Zeng ◽  
Jixun Zhan
Keyword(s):  
Gene Cluster ◽  
Genome Sequencing ◽  
Fermentation Broth ◽  
Biosynthetic Gene Cluster ◽  
The Novel ◽  
Biosynthetic Gene ◽  
Content Type ◽  
Minor Metabolite ◽  
Streptomyces Chromofuscus ◽  
A Minor

ABSTRACTThe 53-kb biosynthetic gene cluster for the novel anticholesterol natural product herboxidiene was identified inStreptomyces chromofuscusATCC 49982 by genome sequencing and gene inactivation. In addition to herboxidiene, a biosynthetic intermediate, 18-deoxy-herboxidiene, was also isolated from the fermentation broth ofS. chromofuscusATCC 49982 as a minor metabolite.

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