Porphyrin Derivatives from a Recombinant Escherichia coli Grown on Chemically Defined Medium

2012 ◽  
Vol 22 (12) ◽  
pp. 1653-1658 ◽  
Author(s):  
Min Ju Lee
Keyword(s):  
Escherichia Coli ◽  
Defined Medium ◽  
Recombinant Escherichia Coli ◽  
Chemically Defined Medium ◽  
Porphyrin Derivatives

scholarly journals Metabolic engineering of Escherichia coli W for isobutanol production on chemically defined medium and cheese whey as alternative raw material

2020 ◽  
Vol 47 (12) ◽  
pp. 1117-1132
Author(s):  
Katharina Novak ◽  
Juliane Baar ◽  
Philipp Freitag ◽  
Stefan Pflügl
Keyword(s):  
Escherichia Coli ◽  
Cheese Whey ◽  
Strain Improvement ◽  
Defined Medium ◽  
Raw Material ◽  
Efficient Production ◽  
Substrate Uptake ◽  
Chemically Defined Medium ◽  
E Coli ◽  
Plasmid Library

AbstractThe aim of this study was to establish isobutanol production on chemically defined medium in Escherichia coli. By individually expressing each gene of the pathway, we constructed a plasmid library for isobutanol production. Strain screening on chemically defined medium showed successful production in the robust E. coli W strain, and expression vector IB 4 was selected as the most promising construct due to its high isobutanol yields and efficient substrate uptake. The investigation of different aeration strategies in combination with strain improvement and the implementation of a pulsed fed-batch were key for the development of an efficient production process. E. coli W ΔldhA ΔadhE Δpta ΔfrdA enabled aerobic isobutanol production at 38% of the theoretical maximum. Use of cheese whey as raw material resulted in longer process stability, which allowed production of 20 g l−1 isobutanol. Demonstrating isobutanol production on both chemically defined medium and a residual waste stream, this study provides valuable information for further development of industrially relevant isobutanol production processes.

CAPSULAR POLYSACCHARIDES OF ESCHERICHIA COLI, TYPES K28A AND K34A

1958 ◽  
Vol 4 (5) ◽  
pp. 505-516 ◽  
Author(s):  
Bill B. Wiley ◽  
Henry W. Scherp
Keyword(s):  
Escherichia Coli ◽  
Defined Medium ◽  
Blood Cultures ◽  
The Other ◽  
Capsular Polysaccharides ◽  
Chemically Defined Medium ◽  
Hexuronic Acid ◽  
Other Hand ◽  
Human Sera

Examination of Escherichia coli isolates from 100 diagnostic stool, urine, or blood cultures yielded 13 mucoid strains, identified by strict biochemical criteria. Specific capsular and agglutination reactions identified four strains as K28A (O group 8), four as K34A (O group 9), and two as K42A, whereas three strains were serologically distinct from each other and from the recorded A types.Polysaccharides, identified as the respective capsular materials by inhibition of the specific capsular reactions, were isolated from a K28A strain and a K34A strain, grown in a chemically defined medium. The serological individuality of these polysaccharides correlated with a difference in their component monosaccharides, as determined by chromatography and colorimetry. Both contained galactose, glucose, mannose, and from 10 to 14% of a hexuronic acid, but the K28A polysaccharide contained in addition 13% of fucose.Normal human sera collected in the Rochester area contained antibodies to the K28A and K34A polysaccharides very infrequently and in very low titer. On the other hand, two thirds of such sera contained O group 8 antibody and the other third contained O group 9 antibody, both in much higher titer.

Minimum nutritional requirements for growth of host-independent derivatives of Bdellovibrio bacteriovorus strain 109 Davis

1974 ◽  
Vol 20 (2) ◽  
pp. 263-265 ◽  
Author(s):  
Edward E. Ishiguro
Keyword(s):  
Escherichia Coli ◽  
Growth Factors ◽  
Carbon Source ◽  
Defined Medium ◽  
Nutritional Requirements ◽  
Chemically Defined Medium ◽  
Carbon Source Utilization ◽  
Bdellovibrio Bacteriovorus ◽  
Derivatives Of ◽  
I Cells

A chemically defined medium for host-independent (H-I) derivatives of Bdellovibrio bacteriovorus strain 109 Davis has been developed. Carbon-source utilization studies indicated that H-I cells can assimilate a wider variety of compounds than host-dependent bdellovibrios. The defined medium did not support growth of freshly isolated H-I strains which required unidentified growth factors present in Escherichia coli cell-free extracts and in yeast extract.

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