Tissue Extraction Methods for Metabolic Profiling of a Freshwater Bivalve,Elliptio complanata(Lightfoot, 1786)

2016 ◽  
Vol 33 (2) ◽  
pp. 185-194 ◽  
Author(s):  
Jennifer L. Hurley-Sanders ◽  
Michael K. Stoskopf ◽  
Stacy A. C. Nelson ◽  
William Showers ◽  
J. Mac Law ◽  
...  
Keyword(s):  
Metabolic Profiling ◽  
Extraction Methods ◽  
Freshwater Bivalve ◽  
Tissue Extraction ◽  
Elliptio Complanata

scholarly journals Evaluation of a nonlethal technique for hemolymph collection in Elliptio complanata, a freshwater bivalve (Mollusca: Unionidae)

2005 ◽  
Vol 65 ◽  
pp. 159-165 ◽  
Author(s):  
LL Gustafson ◽  
MK Stoskopf ◽  
AE Bogan ◽  
W Showers ◽  
TJ Kwak ◽  
...  
Keyword(s):  
Freshwater Bivalve ◽  
Elliptio Complanata

Energy distribution in biomass estimates within a freshwater bivalve community

1982 ◽  
Vol 60 (11) ◽  
pp. 2753-2756 ◽  
Author(s):  
C. G. Paterson
Keyword(s):  
Energy Distribution ◽  
New Brunswick ◽  
Standing Stock ◽  
Freshwater Bivalve ◽  
Elliptio Complanata ◽  
Stock Biomass ◽  
Shell Fragments

In an association of the bivalves Elliptio complanata, Anodonta cataracta, and Lampsilis ochracea in a New Brunswick lake, the distribution of standing stock biomass is among tissue (64.6%), shells of living individuals (19.4%), and empty shells and shell fragments (16.0%). Failure to consider the latter two energy compartments would result in serious underestimates of standing stock biomass.

Function of the Neural Sheath in Marine and Freshwater Molluscs. Evidence for Restriction of Sodium Loss in Freshwater Species

1972 ◽  
Vol 56 (2) ◽  
pp. 433-439
Author(s):  
BETTY M. TWAROG ◽  
T. HIDAKA
Keyword(s):  
Action Potentials ◽  
Marine Bivalve ◽  
Common Denominator ◽  
Freshwater Species ◽  
Freshwater Bivalve ◽  
Elliptio Complanata ◽  
Freshwater Molluscs ◽  
Freshwater Clams ◽  
Sodium Loss ◽  
Neural Sheath

1. Action potentials were observed in cerebrovisceral connectives of a marine bivalve, Mytilus edulis L., and two species of freshwater bivalve, Anodonta cataracta and Elliptio complanata. 2. In Mytilus nerve, responses to stimulation in both the intact and the de-sheathed nerve were blocked after two minutes in a sodium-free saline. Restoration of normal sodium levels reversed the block within 5 min. 3. In nerves of the freshwater species, Anodonta and Elliptio, the action potential of the sheathed nerve altered very slightly after one hour of stimulation in a sodium-free solution. The completely de-sheathed nerve, however, was blocked within one minute in sodium-free saline. The block was rapidly reversible. 4. It is concluded, contrary to conclusions of previous investigators, that the neural sheath in freshwater clams is just as vital to restriction of sodium loss as it is in amphibia and insects, and it would seem logical to seek out a common denominator of sheath function. Several possible mechanisms are put forth and discussed.

scholarly journals Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia

2014 ◽  
Vol 32 (1) ◽  
pp. 7-13
Author(s):  
Jorge Evelio Angel ◽  
Erick Geovanni Hernández ◽  
Néstor Andrés Herrera ◽  
Linda Yhiset Gómez ◽  
Ángela Patricia Castro ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Plant Tissue ◽  
Extraction Method ◽  
Extraction Methods ◽  
Quality Parameters ◽  
Validation Process ◽  
Citrus Plant ◽  
Tissue Extraction ◽  
Citrus Huanglongbing ◽  
Dna Extraction Methods

Four DNA citrus plant tissue extraction protocols and three methods of DNA extraction from vector psyllid Diaphorina citriKuwayama (Hemiptera: Psyllidae) were compared as part of the validation process and standardization for detection of huanglongbing (HLB). The comparison was done using several criterias such as integrity, purity and concentration. The best quality parameters presented in terms of extraction of DNA from plant midribs tissue of citrus, were cited by Murray and Thompson (1980) and Rodríguez et al. (2010), while for the DNA extraction from psyllid vectors of HLB, the best extraction method was suggested by Manjunath et al.(2008).

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