Proteomic Fingerprinting Discriminates Cryptic Gastropod Species

Malacologia ◽  
2020 ◽  
Vol 63 (1) ◽  
pp. 131
Author(s):  
Thomas Wilke ◽  
Jasmin Renz ◽  
Torsten Hauffe ◽  
Diana Delicado ◽  
Janna Peters
Keyword(s):  
Gastropod Species ◽  
Proteomic Fingerprinting

scholarly journals Premating barriers in young sympatric snail species

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Arina L. Maltseva ◽  
Marina A. Varfolomeeva ◽  
Arseniy A. Lobov ◽  
Polina O. Tikanova ◽  
Egor A. Repkin ◽  
...  
Keyword(s):  
Gene Flow ◽  
Snail Species ◽  
Sympatric Populations ◽  
Gastropod Species ◽  
Shell Size ◽  
Closely Related Species ◽  
Partial Canonical Correspondence Analysis ◽  
In The Wild ◽  
Isolation Index

AbstractSympatric coexistence of recently diverged species raises the question of barriers restricting the gene flow between them. Reproductive isolation may be implemented at several levels, and the weakening of some, e.g. premating, barriers may require the strengthening of the others, e.g. postcopulatory ones. We analysed mating patterns and shell size of mates in recently diverged closely related species of the subgenus Littorina Neritrema (Littorinidae, Caenogastropoda) in order to assess the role of premating reproductive barriers between them. We compared mating frequencies observed in the wild with those expected based on relative densities using partial canonical correspondence analysis. We introduced the fidelity index (FI) to estimate the relative accuracy of mating with conspecific females and precopulatory isolation index (IPC) to characterize the strength of premating barriers. The species under study, with the exception of L. arcana, clearly demonstrated preferential mating with conspecifics. According to FI and IPC, L. fabalis and L. compressa appeared reliably isolated from their closest relatives within Neritrema. Individuals of these two species tend to be smaller than those of the others, highlighting the importance of shell size changes in gastropod species divergence. L. arcana males were often found in pairs with L. saxatilis females, and no interspecific size differences were revealed in this sibling species pair. We discuss the lack of discriminative mate choice in the sympatric populations of L. arcana and L. saxatilis, and possible additional mechanisms restricting gene flow between them.

scholarly journals Unveiling Putative Functions of Mucus Proteins and Their Tryptic Peptides in Seven Gastropod Species Using Comparative Proteomics and Machine Learning-Based Bioinformatics Predictions

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3475
Author(s):  
Viroj Tachapuripunya ◽  
Sittiruk Roytrakul ◽  
Pramote Chumnanpuen ◽  
Teerasak E-kobon
Keyword(s):  
Bioactive Peptides ◽  
Nearest Neighbor ◽  
Peptide Identification ◽  
Comparative Proteomics ◽  
Mass Spectrometric ◽  
Functional Categories ◽  
K Nearest Neighbor ◽  
Gastropod Species ◽  
Bioactive Properties ◽  
Sds Page

Gastropods are among the most diverse animals. Gastropod mucus contains several glycoproteins and peptides that vary by species and habitat. Some bioactive peptides from gastropod mucus were identified only in a few species. Therefore, using biochemical, mass spectrometric, and bioinformatics approaches, this study aimed to comprehensively identify putative bioactive peptides from the mucus proteomes of seven commonly found or commercially valuable gastropods. The mucus was collected in triplicate samples, and the proteins were separated by 1D-SDS-PAGE before tryptic digestion and peptide identification by nano LC-MS/MS. The mucus peptides were subsequently compared with R scripts. A total of 2818 different peptides constituting 1634 proteins from the mucus samples were identified, and 1218 of these peptides (43%) were core peptides found in the mucus of all examined species. Clustering and correspondence analyses of 1600 variable peptides showed unique mucous peptide patterns for each species. The high-throughput k-nearest neighbor and random forest-based prediction programs were developed with more than 95% averaged accuracy and could identify 11 functional categories of putative bioactive peptides and 268 peptides (9.5%) with at least five to seven bioactive properties. Antihypertensive, drug-delivering, and antiparasitic peptides were predominant. These peptides provide an understanding of gastropod mucus, and the putative bioactive peptides are expected to be experimentally validated for further medical, pharmaceutical, and cosmetic applications.

MicroCT protocols for scanning of embryos and juvenile Hexaplex trunculus v1

2021 ◽  
Author(s):  
Eva Chatzinikolaou ◽  
Kleoniki Keklikoglou
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
3D Imaging ◽  
Imaging Technique ◽  
Ct Scanning ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Gastropod Species ◽  
Biological Studies ◽  
Hexaplex Trunculus

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. This protocol aims to standardise micro-CT scanning procedures for embryos and juveniles of the marine gastropod species Hexaplex trunculus.

scholarly journals Bivalves and Gastropods of the Gulf of Tehuantepec, Mexico: A Checklist of Species with Notes on Their Habitat and Local Distribution

2009 ◽  
Vol 2009 ◽  
pp. 1-12 ◽  
Author(s):  
Eduardo Ríos-Jara ◽  
Ceciel-M. Navarro-Caravantes ◽  
Cristian-M. Galván-Villa ◽  
Ernesto Lopez-Uriarte
Keyword(s):  
Continental Shelf ◽  
Coastal Lagoons ◽  
Taxonomic Composition ◽  
Total Count ◽  
Bivalve Species ◽  
Local Distribution ◽  
Gastropod Species ◽  
Gulf Of Tehuantepec ◽  
Shallow Subtidal

The taxonomic composition of 160 species of bivalves and gastropods recorded in the Gulf of Tehuantepec is presented with information on their habitat and distribution along 10 different localities of the shoreline and 42 stations of the continental shelf. The species were on sandy and rocky beaches, coastal lagoons, estuaries, mangroves, rocky breakwaters of ports, and shallow subtidal areas (14–47 m depth). A total of 78 bivalve species and 82 gastropod species were recorded. Most of these were associated with sandy and rocky beaches and breakwaters of ports. The estuaries host 30 species and the coastal lagoons only two. In the shallow subtidal there were 18 gastropod species and 40 bivalve species representing 36.3% of all. This study adds 24 bivalve species and 29 gastropod species not recorded in previous studies for a total count of 213 species (102 bivalves and 111 gastropods) for Gulf of Tehuantepec.

scholarly journals Imposex in endemic volutid from Northeast Brazil (Mollusca: Gastropoda)

2008 ◽  
Vol 51 (5) ◽  
pp. 1065-1069 ◽  
Author(s):  
Ítalo Braga de Castro ◽  
Carlos Augusto Oliveira de Meirelles ◽  
Helena Matthews-Cascon ◽  
Cristina de Almeida Rocha-Barreira ◽  
Pablo Penchaszadeh ◽  
...  
Keyword(s):  
First Record ◽  
Reproductive Organs ◽  
Organotin Compounds ◽  
Northeast Brazil ◽  
Gastropod Species ◽  
Antifouling Paints ◽  
Tin Compounds ◽  
Stramonita Haemastoma ◽  
Mollusk Species ◽  
Female Reproductive Organs

Imposex is characterized by the development of masculine sexual organs in neogastropod females. Almost 120 mollusk species are known to present imposex when exposed to organic tin compounds as tributyltin (TBT) and triphenyltin (TPT). These compounds are used as biocide agents in antifouling paints to prevent the incrustations on boats. Five gastropod species are known to present imposex in Brazil: Stramonita haemastoma, Stramonita rustica, Leucozonia nassa, Cymathium parthenopeum and Olivancillaria vesica. This paper reports the first record of imposex observed in the endemic gastropod Voluta ebraea from Pacheco Beach, Northeast Brazil. Animals presenting imposex had regular female reproductive organs (capsule gland, oviduct and sperm-ingesting gland) and an abnormal penis. As imposex occurs in mollusks exposed to organotin compounds typically found at harbors, marinas, shipyards and areas with high shipping activities, probably contamination of Pacheco Beach is a consequence of a shipyard activity located in the nearest areas.

Intensified grazing affects endemic plant and gastropod diversity in alpine grasslands of the Southern Carpathian mountains (Romania)

Biologia ◽  
2007 ◽  
Vol 62 (4) ◽  
Author(s):  
Bruno Baur ◽  
Cristina Cremene ◽  
Gheorghe Groza ◽  
Anatoli Schileyko ◽  
Anette Baur ◽  
...  
Keyword(s):  
Species Richness ◽  
Vascular Plant ◽  
Calcareous Soils ◽  
Acid Soils ◽  
Endemic Plant ◽  
Alpine Grasslands ◽  
Gastropod Species ◽  
Mountain Area ◽  
The Carpathians ◽  
Steep Slopes

AbstractAlpine grasslands in the Southern Carpathian Mts, Romania, harbour an extraordinarily high diversity of plants and invertebrates, including Carpathic endemics. In the past decades, intensive sheep grazing has caused a dramatic decrease in biodiversity and even led to eroded soils at many places in the Carpathians. Because of limited food resources, sheep are increasingly forced to graze on steep slopes, which were formerly not grazed by livestock and are considered as local biodiversity hotspots. We examined species richness, abundance and number of endemic vascular plants and terrestrial gastropods on steep slopes that were either grazed by sheep or ungrazed by livestock in two areas of the Southern Carpathians. On calcareous soils in the Bucegi Mts, a total of 177 vascular plant and 19 gastropod species were recorded. Twelve plant species (6.8%) and three gastropod species (15.8%) were endemic to the Carpathians. Grazed sites had lower plant and gastropod species richness than ungrazed sites. Furthermore, grazed sites harboured fewer gastropod species endemic to the Carpathians than ungrazed sites. On acid soils in the Fagaras Mts, a total of 96 vascular plant and nine gastropod species were found. In this mountain area, however, grazed and ungrazed sites did not differ in species richness, abundance and number of endemic plant and gastropod species. Our findings confirm the high biodiversity of grasslands on steep slopes in the Southern Carpathian Mts and caution against increasing grazing pressure in these refuges for relic plants and gastropods as well as for other invertebrates.

scholarly journals Analysis of Gastropod Diversity as a Bioindicator of Waters in Situ Ciburuy Padalarang, West Bandung Regency, West Java, Indonesia

2021 ◽  
pp. 10-19
Author(s):  
Erwin Theofilius ◽  
Zahidah Hasan ◽  
Asep Agus Handaka ◽  
Herman Hamndani
Keyword(s):  
Water Quality ◽  
Species Diversity ◽  
Sampling Method ◽  
Diversity Index ◽  
Research Area ◽  
Survey Method ◽  
Gastropod Species ◽  
Evenness Index

This study conducted to determine the water quality of Situ Ciburuy based on the structure of the gastropod community as a bioindicator. The survey method used in this study based on collecting data directly at the research area (purpose sampling method).  For instance, species and density of gastropods were descriptively analyzed, using diversity index and evenes index. For instance, The findings showed that level of water quality in  Situ Ciburuy based on gastropods bioindicators was lightly polluted. That is indicated by the low to moderate diversity of gastropods, which ranges from 1.31-1.98. The Shannon evenness index (0,73-0.95) revealed low gastropod species diversity in Situ Ciburuy, indicating low evenness of gastropod in Situ Ciburuy.

scholarly journals First record of subterranean rissoidean gastropod assemblages in Southeast Asia (Mollusca, Gastropoda, Pomatiopsidae)

2018 ◽  
Vol 25 ◽  
pp. 9-34
Author(s):  
Jozef Grego
Keyword(s):  
Southeast Asia ◽  
New Genus ◽  
First Record ◽  
Karst Spring ◽  
Gastropod Species ◽  
The Third ◽  
Freshwater Gastropod

In February 2017 we investigated several caves and karstic springs in Laos for the presence of underground freshwater gastropod species. We report previously unrecorded freshwater gastropod assemblages in the largest cave in Laos, Tham Khon Dôn, and in the third largest cave, Pha Soung, in Khammouane Province, with single finds in Na Li Cave (Khammouane Province), an unnamed cave near Vieng Thong (Bolikhamsay Province) and a small karst spring near Phonsavan (Xianghouan Province). All 15 species recorded and described herein are new to science. Four species are assigned to the new genus Pseudoiglica: P. pseudoiglica sp. n., P. olsavskyi sp. n., P. kameniari sp. n., and P. phonsavanica sp. n. Three species are assigned to the new genus Thamkhondonia: T. moureti sp. n., T. vacquiei sp. n., and T. smidai sp. n. Eight species are assigned to the genus Tricula Benson, 1843: T. valenasi sp. n., T. davisi sp. n., T. spelaea sp. n., T. lenahani sp. n., T. reischuetzorum sp. n., T. phasoungensis sp. n., T. bannaensis sp. n., and T. viengthongensis sp. n.

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