Eukaryotic Expression and Biochemical Characterization of Intestinal Mucin SeIIM8 fromSpodoptera exigua(Hübner) (Lepidoptera: Noctuidae)

2012 ◽  
Vol 20 (2) ◽  
pp. 252-258
Author(s):  
X.N. Li ◽  
R.J. Li ◽  
D.Q. Xu ◽  
W. Guo ◽  
W.M. Sun ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Eukaryotic Expression ◽  
Intestinal Mucin ◽  
Lepidoptera Noctuidae

Biochemical characterization of α- and β-glucosidases in alimentary canal, salivary glands and haemolymph of the rice green caterpillar, Naranga aenescens M. (Lepidoptera: Noctuidae)

Biologia ◽  
2012 ◽  
Vol 67 (6) ◽  
Author(s):  
Ameneh Asadi ◽  
Mohammad Ghadamyari ◽  
Reza Sajedi ◽  
Jalal Sendi ◽  
Mehrdad Tabari
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Alimentary Canal ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Biochemical Properties ◽  
Maximum Activity ◽  
Lepidoptera Noctuidae ◽  
Optimal Ph

AbstractThe biochemical properties of α- and β-glucosidase in salivary glands, alimentary canal and haemolymph of Naranga aenescens larvae, one of the most damaging pests of the rice crop in Iran, were investigated. The specific activity of α-glucosidases were 3.88, 2.74 and 1.58 μmol/min per mg protein in the alimentary canal, salivary glands and haemolymph of last instar larvae, respectively. The specific activity of β-glucosidases were 1.27, 0.077 and 0.414 μmol/min per mg protein in the alimentary canal, salivary glands and haemolymph of last instar larvae, respectively. The optimal pH for α-glucosidases were 6.0, 6.0–8.0 and 6.0 and the maximum activity for β-glucosidases were obtained at pH 6.0, 5.0–7.0 and 5.0 in alimentary canal, salivary glands and haemolymph, respectively. The optimum temperatures for β-glucosidases were determined at 55°C in alimentary canal, 35–45°C in salivary glands and 55°C in haemolymph, whereas the α-glucosidases reached their optimum at 45°C in all three tissues. Effect of metal ions on the activity of α- and β-glucosidases showed that K+ (20 mM) and Mg2+ (10 and 20 mM) increased N. aenescens α- and β-glucosidases activities from salivary glands, while Ca2+ increased α- and β-glucosidases activities in haemolymph. In the presence of Fe2+, Mn2+, Hg+ and Zn2+ (10, 20 mM) and Hg2+ (20 mM), these enzymes from all tissues were completely inactivated. K m values were estimated for the α-glucosidases as 3.96, 0.547 and 3.084 mM and for β-glucosidases as 1.93, 1.014 and 1.93 mM in the alimentary canal, salivary gland and haemolymph, respectively. The zymogram analyses of N. aenescens crude extracts indicated the presence of at least two isoforms for α-glucosidase and one isoform for β-glucosidase.

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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