Cytokine production inhibition from human peripheral blood monocytes by adding procaterol and aminophylline.

Factor XIII is the fibrin-stabilizing factor that covalently cross- links fibrin monomers to form a highly organized, stable fibrin clot. The plasma form of factor XIII is a heterodimer, a2b2, consisting of two a-chains and two b-chains; the intracellular form, such as in platelets and placenta, is a dimer, a2, consisting of a-chains only. The catalytic function of factor XIII, a transglutaminase, resides in the a-chain. To address questions regarding sites of synthesis of factor XIII a-chain, an EcoRI restriction fragment from the protein- coding region of the factor XIII a-chain cDNA was used as a probe for Northern blot analysis. The cDNA probe showed hybridization with a single approximately 4.0-kilobase (kb) message in poly (A)+ mRNA prepared from normal human peripheral blood monocytes and normal human liver. The results demonstrate conclusively that factor XIII a-chains are actively synthesized in circulating monocytes and in liver. To our knowledge, these data represent the first demonstration of synthesis of any blood coagulation factor in primary uncultured and unstimulated monocytes or macrophage cells.

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Peroxynitrite reduces endotoxin-induced tissue factor expression in human peripheral blood monocytes

Critical Care ◽

10.1186/cc29 ◽

1997 ◽

Vol 1 (Suppl 1) ◽

pp. P023

Author(s):

M Gerlach ◽

D Keh ◽

S Spielmann ◽

T Kerner ◽

R Peter ◽

...

Keyword(s):

Tissue Factor ◽

Peripheral Blood ◽

Human Peripheral Blood ◽

Tissue Factor Expression ◽

Blood Monocytes ◽

Peripheral Blood Monocytes ◽

Factor Expression

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Evidence for specific annexin I-binding proteins on human monocytes

Biochemical Journal ◽

10.1042/bj3160593 ◽

1996 ◽

Vol 316 (2) ◽

pp. 593-597 ◽

Cited By ~ 38

Author(s):

Nicolas J. GOULDING ◽

L PAN ◽

Kathleen WARDWELL ◽

Veronica C. GUYRE ◽

Paul M. GUYRE

Keyword(s):

Monoclonal Antibody ◽

Peripheral Blood ◽

Binding Proteins ◽

Human Peripheral Blood ◽

Flow Cytometric Analysis ◽

Blood Monocytes ◽

Surface Binding ◽

Peripheral Blood Monocytes ◽

Cell Functions ◽

Annexin I

Recombinant human annexin I and a monoclonal antibody specific for this protein (mAb 1B) were used to investigate surface binding of this member of the annexin family of proteins to peripheral blood monocytes. Flow cytometric analysis demonstrated trypsin-sensitive, saturable binding of annexin I to human peripheral blood monocytes but not to admixed lymphocytes. A monoclonal antibody that blocks the anti-phospholipase activity of annexin I also blocked its binding to monocytes. These findings suggest the presence of specific binding sites on monocytes. Furthermore, surface iodination, immunoprecipitation and SDS/PAGE analysis were used to identify two annexin I-binding proteins on the surface of monocytes with molecular masses of 15 kDa and 18 kDa respectively. The identification and characterization of these annexin I-binding molecules should help us to better understand the specific interactions of annexin I with monocytes that lead to down-regulation of pro-inflammatory cell functions.

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