scholarly journals Assessment of oxidative stress, genotoxicity and histopathological responses in the digestive gland of Ruditapes decussatus collected from northern Tunisian lagoons

2020 ◽  
Vol 84 (4) ◽  
pp. 403-420
Author(s):  
Safa Bejaoui ◽  
Imen Rabeh ◽  
Khaoula Telahigue ◽  
Mariem Tir ◽  
Imene Chetoui ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Dna Damage ◽  
Protein Oxidation ◽  
Warm Season ◽  
Ruditapes Decussatus ◽  
Anthropogenic Pressure ◽  
Antioxidant Defence ◽  
Histological Alterations ◽  
Contaminated Area

The aim of the present study was to investigate the combined effects of seasonality and anthropogenic pressure on a battery of oxidative stress, lipid peroxidation, protein oxidation, DNA damage and histological alterations in the native clam Ruditapes decussatus collected from a less contaminated area (LCA), Ghar El Melh, a moderately contaminated area (MCA), the North Lake, and a highly contaminated area (HCA), the South Lake, all located in the southern Mediterranean Sea. The accumulation of cadmium, lead, copper, iron and zinc was higher in the digestive glandsof clams collected from the MCA and the HCAthan in those from the LCA, particularly during the warm season. Our results reveal that metallothionein, lipid peroxidation, protein oxidation levels and antioxidant defence systems were higher, while acetylcholinesterase activity was lower, in clams from the MCAand HCAthan in those from the LCA. The results also indicate that clams from the MCA and the HCAare characterized by histological alterations and DNA damage. In conclusion, the evident changes of antioxidant defence systems and macromolecules between the studied lagoons reveal the perturbation of the physiological states of clams from polluted sites thatcope with seasonal changes and trace element accumulations.

scholarly journals Relation between Gastric Cancer and Protein Oxidation, DNA Damage, and Lipid Peroxidation

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Yongsheng Ma ◽  
Lin Zhang ◽  
Shengzhong Rong ◽  
Hongyan Qu ◽  
Yannan Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gastric Cancer ◽  
Lipid Peroxidation ◽  
Dna Damage ◽  
Protein Oxidation ◽  
Antioxidant Defense System ◽  
Protein Carbonyl ◽  
Treatment And Prevention ◽  
Healthy Control ◽  
The Relationship

Objects.The aim of this study is to evaluate protein oxidation, DNA damage, and lipid peroxidation in patients with gastric cancer and to investigate the relationship between oxidative stress and gastric cancer.Methods. We investigated changes in serum protein carbonyl (PC), advanced oxidation protein products (AOPP), and 3-nitrotyrosine (3-NT) levels, as indicators of protein oxidation, serum 8-hydroxydeoxyguanosine (8-OHdG), as a biomarker of DNA damage, and malondialdehyde (MDA), conjugated diene (CD), 4-hydroxynonenal (4-HNE), and 8-ISO-prostaglandinF2α(8-PGF) in serum, as lipid peroxidation markers in gastric cancer (GC) patients and healthy control.Results. Compared with control, a statistically significant higher values of 8-OHdG, PC, AOPP, and 3-NT were observed in the GC patients (P<0.05). The products of lipid peroxidation, MDA, CD, 4-HNE, and 8-PGF, were significantly lower in the GC patients compared to those of control (P<0.05). In addition, the products of oxidative stress were similar between the Helicobacter pylori positive and the negative subgroups of GC patients.Conclusions. GC patients were characterized by increased protein oxidation and DNA damage, and decreased lipid peroxidation. Assessment of oxidative stress and augmentation of the antioxidant defense system may be important for the treatment and prevention of gastric carcinogenesis.

scholarly journals Protective activity of Hertia cheirifolia extracts against DNA damage, lipid peroxidation and protein oxidation

2016 ◽  
Vol 55 (1) ◽  
pp. 330-337 ◽  
Author(s):  
Seoussen Kada ◽  
Hamama Bouriche ◽  
Abderrahmane Senator ◽  
Ibrahim Demirtaş ◽  
Tevfik Özen ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Dna Damage ◽  
Protein Oxidation ◽  
Protective Activity

scholarly journals Strawberry Intake Ameliorates Oxidative Stress and Decreases GABA Levels Induced by High-Fat Diet in Frontal Cortex of Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 70 ◽  
Author(s):  
Cuauhtémoc Sandoval-Salazar ◽  
Cecilia Oviedo-Solís ◽  
Edmundo Lozoya-Gloria ◽  
Herlinda Aguilar-Zavala ◽  
Martha Solís-Ortiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Oxidative Damage ◽  
Frontal Cortex ◽  
Protein Oxidation ◽  
High Fat Diet ◽  
High Fat ◽  
Gaba Concentration ◽  
Obese Rats ◽  
Lipid And Protein Oxidation

It has been proposed that there is a correlation between high-fat diet (HFD), oxidative stress and decreased γ-aminobutyric acid (GABA) levels, but this has not been thoroughly demonstrated. In the present study, we determined the effects of strawberry extract intake on the oxidative stress and GABA levels in the frontal cortex (FC) of obese rats. We observed that an HFD increased lipid and protein oxidation, and decreased GABA levels. Moreover, UV-irradiated strawberry extract (UViSE) decreased lipid peroxidation but not protein oxidation, whereas non-irradiated strawberry extract (NSE) reduced protein oxidation but not lipid peroxidation. Interestingly, NSE increased GABA concentration, whereas UViSE was not as effective. In conclusion, our results suggest that an HFD increases oxidative damage in the FC, whereas strawberry extract intake may ameliorate the disturbances associated with HFD-induced oxidative damage.

scholarly journals Oxidative Stress Induction by Lead in Leaves of Radish (Raphanus sativus) Seedlings

2011 ◽  
Vol 3 (4) ◽  
pp. 93-99 ◽  
Author(s):  
Nadjet BITEUR ◽  
Abdelkader AOUES ◽  
Omar KHAROUBI ◽  
Miloud SLIMANI
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Oxidation ◽  
Raphanus Sativus ◽  
Lead Concentration ◽  
Control Treatment ◽  
Enzymatic Antioxidants ◽  
Carotenoid Concentration ◽  
Hydroponic System ◽  
Physiological Processes

Oxidative stress was induced by lead acetate (Pb) in Raphanus sativus seedlings grown in a hydroponic system using sand as substrate. Thirty day old acclimated seeds were treated for 7 days with five Pb levels (0 as control, 100, 200, 500 and 1000 mg l-1). Parameters such as growth, oxidative damage markers (lipid peroxidation, protein oxidation and hydrogen peroxide contents) and enzymatic activities of catalase (CAT) and peroxidase (POD) were investigated. Lead concentration in plant tissues increased with increasing of Pb levels. Shoot fresh weight, chlorophyll and carotenoid concentration were significantly decreased at 100 mg l-1 Pb. Lipid peroxidation, protein oxidation and H2O2 levels were increased at 500 and 1000 mg l-1 Pb compared to control treatment, in shoots. Peroxidase activity showed a straight correlation with H2O2 concentration, whereas CAT activity decreased only in shoots. These changes in enzymatic and non-enzymatic antioxidants showed that the Pb exposition had a significant disturbance on Raphanus sativus plantlets and affect the biochemical and physiological processes.

Lipid Peroxidation, Protein Oxidation, Gelatinases, and Their Inhibitors in a Group of Adults with Obesity

2019 ◽  
Vol 51 (06) ◽  
pp. 389-395 ◽  
Author(s):  
Gregorio Caimi ◽  
Baldassare Canino ◽  
Maria Montana ◽  
Caterina Urso ◽  
Vincenzo Calandrino ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Protein Oxidation ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
The Body ◽  
Control Group ◽  
The Matrix ◽  
Markers Of Oxidative Stress ◽  
Obese Subjects

AbstractThe association between obesity and cardiovascular diseases has a multifactorial pathogenesis, including the synthesis of inflammatory molecules, the increase in oxidative stress and the dysregulation of the matrix metalloprotease (MMP) concentration and activity. In a group of adults with obesity, divided in 2 subgroups according to the body mass index (BMI), we examined lipid peroxidation, expressed as thiobarbituric acid-reactive substances (TBARS), protein oxidation, expressed as protein carbonyl groups (PCs), plasma gelatinases (MMP-2 and MMP-9), and their tissue inhibitors (TIMP-1 and TIMP-2). In the whole group, as well as in the 2 subgroups (with BMI 30–35 or BMI>35) of obese subjects, we observed an increase in TBARS, PCs, MMP-2, and MMP-9, and also TIMP-1 and TIMP-2 in comparison with the control group. A positive correlation between TBARS and PCs emerged in obese subjects and persisted after dividing obese subjects according to BMI. The correlation between MMP-2 and TIMP-2 was not statistically significant, while a significant correlation was present between MMP-9 and TIMP-1. The correlations between the markers of oxidative stress (TBARS and PCs) and those of the MMP/TIMP profile indicated a more marked influence of protein oxidation on MMPs and TIMPs in comparison with TBARS. The innovative aspect of our study was the simultaneous evaluation of oxidative stress markers and MMP/TIMP profile in adult obese subjects. We observed significant alterations and correlations that may negatively influence the clinical course of the disease.

scholarly journals Impact of dietary oxidized protein on oxidative status and performance in growing pigs

2020 ◽  
Vol 98 (5) ◽  
Author(s):  
Carl A Frame ◽  
Erika Johnson ◽  
Logan Kilburn ◽  
Elisabeth Huff-Lonergan ◽  
Brian J Kerr ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Lipid Oxidation ◽  
Growth Performance ◽  
Linear Relationship ◽  
Dietary Protein ◽  
Protein Oxidation ◽  
Oxidative Status ◽  
Growing Pigs ◽  
Positive Linear Relationship

Abstract Rendered products from the meat industry can provide economical quality sources of proteins to the animal and feed industry. Similar to lipids, rendered proteins are susceptible to oxidation, yet the stability of these proteins is unclear. In addition, interest in understanding how oxidative stress can impact efficiency in production animals is increasing. Recent studies show that consumption of oxidized lipids can lead to a change in the oxidative status of the animal as well as decreases in production efficiency. To date, little is known about how consumption of oxidized proteins impacts oxidative status and growth performance. The objectives of this study were to determine if feeding diets high in oxidized protein to growing pigs would: 1) impact growth performance and 2) induce oxidative stress. Thirty pigs (42 d old; initial body weight [BW] 12.49 ± 1.45 kg) were randomly assigned to one of three dietary treatments with increasing levels of oxidized protein. Spray-dried bovine plasma was used as the protein source and was either unheated upon arrival, heated at 45 °C for 4 d, or heated at 100 °C for 3 d. Diets were fed for 19 d and growth performance was measured. Blood plasma (days 0 and 18), jejunum, colon, and liver tissues (day 19) were collected to analyze for markers of oxidative stress (e.g., protein oxidation, lipid oxidation, DNA damage, and glutathione peroxidase activity). Average daily gain (ADG;P &lt; 0.01) and average daily feed intake (ADFI;P &lt; 0.01) had a positive linear relationship to increased protein oxidation, but there was no effect on gain to feed ratio. Furthermore, protein (P = 0.03) and fat (P &lt; 0.01) digestibility were reduced with increased protein oxidation in the diet. Crypt depth showed a positive linear relationship with dietary protein oxidation levels (P = 0.02). A trend was observed in liver samples where pigs fed the plasma heated to 45 °C had increased lipid oxidation compared with pigs fed the plasma either unheated or heated to 100 °C (P = 0.09). DNA damage in the jejunum tended to have a linear relationship with the dietary protein oxidation level (P = 0.07). Even though results suggest dietary oxidized protein did not induce oxidative stress during short-term feeding, differences in performance, gut morphology, and digestibility are likely a result of reduced protein availability.

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