scholarly journals Production of lipids and natural antioxidants from passion fruit seeds

2020 ◽  
Vol 71 (4) ◽  
pp. 385
Author(s):  
C. C. Reis ◽  
A. M.G.N. Mamede ◽  
A. Soares ◽  
S. P. Freitas

The wild passion fruit species Passiflora setacea, Passiflora alata, and Passiflora tenuifila are native to the Brazilian biomass. The seed waste generated from the extraction of passion fruit juice contains functional polyunsaturated fatty acids and phenolic compounds. The aims of this study were to obtain lipids and natural antioxidants from passion fruit seeds. Passion seed oils were extracted using a lab-scale continuous press and their oxidative stability was evaluated using the Rancimat® method. Higher antioxidant extract capacity was observed when using an ethanol-water solution (70:30) at 45 ºC. In these cases, the total phenolic contents expressed as gallic acid equivalents from P. setacea, P. alata, and P. tenuifila cakes were approximately 1800, 600 and 900 mg·100g−1 of extract. Induction periods increased up to two-fold when adding these extracts to their respective seed oil. Therefore, passion fruit seed extract can contribute to increasing the oxidative stability of polyunsaturated oils.

Author(s):  
RASNA GUPTA ◽  
RAM LAKHAN SINGH ◽  
ANKIT GUPTA

Objective: The present study was undertaken to investigate the antioxidative activity of fruit pulp, seed and bark of T. bellerica. Methods: Methanol (70%) and water were used for the extraction, and the respective extracts were analyzed for total phenolic contents along with the antioxidant activities through different assays. Results: Amongst the tested extracts, methanolic extracts were found to be better than aqueous with the highest total phenolic contents (TPC). Methanolic fruit pulp showed lower IC50 for free radical (118.7 µg/ml), superoxide anion radical (77.65 µg/ml) and hydroxyl radical (73.76 µg/ml) scavenging activities. Methanolic fruit pulp also showed lower IC50 for lipid peroxidation (115.6 µg/ml) and ferric thiocyanate (184.98 µg/ml) assay than aqueous extracts in comparison to standard quercetin. Further, correlation between TPC and antioxidant studies revealed that phenolics are mainly responsible for antioxidant activity of T. bellerica. Conclusion: The current study suggests that the methanolic extract of T. bellerica could be a potential source of natural antioxidants for food and pharmaceutical companies.


2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Muhammad Asam Raza ◽  
Rukhsana Kausar ◽  
Faraz Ali Rana ◽  
Muhammad Danish ◽  
Durre Shahwar ◽  
...  

This study was designed to evaluate the antioxidant potential ofLoranthus pulverulentus. Stem bark, leaves, and seeds ofLoranthus pulverulentuswere extracted in methanol:water (90 : 10) and partitioned with n-hexane, chloroform, ethyl acetate, and n-butanol successively using partition chromatography. Total phenolic contents and antioxidant potential were checked using standard protocols. Total phenolic contents of all extracts were determined, using Folin–Ciocalteu reagent, and ranged between 151 ± 2.1 and 396 ± 1.6 for stem bark, 137 ± 0.9 and 430 ± 2.2 for, and 39 ± 0.6 and 231 ± 1.7 for seeds. The antioxidant potential of extracts was evaluated; namely, DPPH, FRAP, and total antioxidant models. The ethyl acetate extract of stem-bark, leaves, and seeds showed the highest activity in DPPH (94.5 ± 2.1%, 96.30 ± 0.9%, and 92.30 ± 1.1%, IC5015.9 ± 0.5 μg, 14.5 ± 0.8, and 102.7 ± 1.3, resp.), FRAP (7.7 ± 0.6, 7.5 ± 0.7 and 6.6 ± 0.7, resp.), and total antioxidant (0.95 ± 0.09, 1.19 ± 0.09, and 0.686 ± 0.08, resp.). Strong correlations were observed between total phenols versus total antioxidant activity, DPPH, and FRAP withR2values ranging from 0.8185 to 0.9951 (stem-bark), 0.6728 to 0.8648 (leaves), and 0.8658 to 0.9910 (seed) which indicated that phenolic contents are the major constituents responsible for antioxidant activity.


2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Farouk Mraihi ◽  
Mohamed Journi ◽  
Jamila Kalthoum Chérif ◽  
Munevver Sokmen ◽  
Atalay Sokmen ◽  
...  

Crataegusfruit is one of most important fruits in Tunisian flora. Some fruits of this genus are edible. This study was undertaken in order to examine the benefits of these fruits in human health and their composition of antioxidants including total polyphenol, flavonoids, proanthocyanidins content, and total anthocyanins. The antioxidative properties of the ultrasonic methanolic extract were assessed by different in vitro methods such as the FRAP, DPPH, andβ-carotene/linoleic acid assay. We concluded that peel fraction of red fruits possessed relatively high antioxidant activity and might be a rich source of natural antioxidants in comparison with the pulp and seed fruit extract. The results also showed that hawthorn yellow fruit presents lower amounts of phenolic content, absence of anthocyanins, and less antioxidant capacity. Most of peel and seed fractions were stronger than the pulp fractions in antioxidant activity based on their DPPH IC50, FRAP values, and results ofβ-carotene/linoleic acid. The total phenolic compounds contents were also highly correlated with the DPPH method and the FRAP assay.


2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Omidreza Firuzi ◽  
Katayoun Javidnia ◽  
Maryam Gholami ◽  
Mohammad Soltani ◽  
Ramin Miri

The antioxidant activities of the methanolic extracts of 9 Salvia species and 15 other Lamiaceae plants growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values ranged form 8.5 to 79.0 μM quercetin equivalents/g dry weight, and IC50 values in the DPPH assay from 115.7 to 1350.2 μg dry weight/mL. Salvia species showed the highest antioxidant activities. S. santolinifolia, S. eremophila and S. palestina, which have not been studied before, were the most active plants. These were more active than the previously studied species from this family, such as S. multicaulis and Marrubium vulgare. S. hydrangea and Gontscharovia popovii also showed high antioxidant activities. FRAP and DPPH assay results showed good correlations with the total phenolic contents of the plants, measured by the Folin-Ciocalteau assay (r2 = 0.925 and 0.799, respectively, p < 0.0001). In conclusion, our study shows that some Lamiaceae plants growing in Iran represent good potential sources of natural antioxidants useful for either prevention or treatment of oxidative stress-related diseases.


2004 ◽  
Vol 10 (4) ◽  
pp. 277-281 ◽  
Author(s):  
G. özkan ◽  
O. Sagdiç ◽  
N. G. Baydar ◽  
H. Baydar

Rosa damascena Mill. is one of the most important Rosa species for the flavour and fragrance industries. The high amount of residues of spent flowers after steam distillation and the potential use of their essential oils as natural antioxidants and antimicrobials lead to determine the antioxidant and antibacterial activities of fresh and spent Rosa damascena flower extracts. The total phenolic contents were 276.02±2.93mg gallic acid equivalent (GAE)/g in FF (fresh flower) extract and 248.97±2.96mg GAE/g in SF (spent flower) extract. FF and SF extracts showed 74.51±1.65 and 75.94±1.72% antiradical activities at 100ppm. The antioxidant activity of FF extract (372.26±0.96mg/g) was higher than that of SF extract (351.36± 0.84mg/g). Antibacterial activity of the extracts was determined by the agar diffusion method against 15 species of bacteria: Aeromonas hydrophila, Bacillus cereus, Enterobacter aerogenes, Enterococcus feacalis, Escherichia coli, Escherichia coli O157:H7, Klebsiella pneumoniae, Mycobacterium smegmatis, Proteus vulgaris, Pseudomonas aeruginosa, Pseudomonas fluorescens, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus aureus and Yersinia enterocolitica. Statistical differences among bacteria were significant at p 0.05. Both extracts were effective against all the bacteria except E. coli O157:H7, although the FF extract was more effective than the SF extract. FF and SF extracts showed the strongest effects against S. enteritidis and M. smegmatis, respectively.


2019 ◽  
Vol 70 (1) ◽  
pp. 295 ◽  
Author(s):  
M. Kiralan ◽  
G. Çalik ◽  
S. Kiralan ◽  
A. Özaydin ◽  
G. Özkan ◽  
...  

The old-pressed oils (CPO) from grape seeds (GSO), flax seeds (FSO) and black cumin seeds (BSO) were analyzed for their fatty acid profiles, tocopherols, total phenolics, bioactives and phenolic compositions. The stability of CPO under thermal oxidation conditions was evaluated. The main fatty acid in FSO was linolenic acid (56.5% of total fatty acids); while GSO and BSO were rich in linoleic acid, which accounted for 66.8 and 56.8%, respectively. GSO was rich in ?-tocopherol (123.0 mg/kg), while ?-tocopherol was a prevalent isomer in FSO and BSO (137.9 and 128.9 mg/kg, respectively). The total phenolic contents in the oils ranged from 554 mg GAE/kg oil (FSO) to 1140 mg GAE/kg oil (BSO). Luteolin, dihydroquercetin and benzoic acids were the dominant bioactives and phenolics in FSO, GSO and BSO, respectively. Based on the oxidative stability index (OSI) value, BSO showed the highest value (6.14 h) among the other oils. The oxidative stability of FSO and BSO were higher than GSO according to peroxide value (PV) and conjugated diene (CD) values of the oils during storage at 60 °C. Hexanal, 2,4-heptadienal and (E,E)-2,4-heptadienal were the major volatile oxidation compounds (VOC) in FSO. Hexanal and (E)-2-heptanal were the main identified VOC in the GSO and BSO under the same oxidation conditions.


Author(s):  
Semra Turan ◽  
Deniz Günal Köroğlu

In this study, oxidative stability of soybean oil (SBO) enriched with ethyl ecetate extracts of olive by-products was investigated. Total phenolic contents, phenolic profiles and antioxidant activities of olive wastewater (OMWW) and olive pomace (OP) extracts were also determined. Total phenolic contents of extracts obtained from OMWW and OP were 134.45 and 281.43 mg gallic acid equivalent (GAE)/g extract, respectively. While antioxidant activities of OMWW extracts in the linoleic acid emulsion were in the range of 85.79 % and 88.54 %, OP extracts had 83.30 % and 90.09 % at different concentrations (0.5, 1, 2 ve 3 mg/mL) after incubation at 37 °C. β-carotene bleaching activities of the extracts at 50 °C were found as 26.80-66.63% in OMWW extracts and 18.76-53.32% in OP extracts, respectively. 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities of OP extracts were higher than those of OMWW extracts and ranged from 30.6% to 87.7% in OP extracts and 16.6% to 54.1% in OMWW extracts at these concentrations. Both the antioxidant and antiradical activities of extracts significantly increased with increased concentration (p


Author(s):  
Khalil Ullah Hasni ◽  
Zul Ilham Zulkiflee Lubes ◽  
Jamaludin Mohamad ◽  
Mahendra Varman

Biodiesel is one of the most promising renewable energy derived from biological sources. Biodiesel is clean and renewable biofuel but its oxidative stability is low. To increase oxidative stability of biodiesel, natural antioxidants are considered as potential compounds to be used. The purpose of the  work was to investigate and determine the antioxidation potential of Brucea javanica seeds by applying different antioxidant assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), metal chelating and ferric reducing antioxidant power (FRAP). Furthermore, Total phenolic content (TPC) and Total flavonoid content (TFC) tests were done. Among the three extracts of Brucea javanica seeds, ethyl acetate extract showed the highest total phenolic content (98.5 ±0.1mg GAE/g dry extract). The highest total flavonoid content (20 ± 0.4mg QAE/g of dry weight) and DPPH inhibition activity 90%. Ethyl acetate extract also exhibited the highest chelating activity with 59% inhibition in the metal chelating assay. However, the Rancimat showed a maximum of 69hour induction period (IP) in biodiesel when gallic acid is added.


2009 ◽  
Vol 1 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Priyanka Chandra ◽  
Daljit Singh Arora

The study was carried outto investigate the antioxidant activity of fungi isolated from soil of different areas of Punjab, India and compare their efficiency with a known antioxidant, ascorbic acid. The antioxidant potential of fungal extracts was quantified by DPPH and reducing power assay. Total phenolic contents were estimated using Folins-Ciocalteau (FC) reagent. Out of 113 fungal isolates selected, 51 were having antioxidant potential and these were further assayed quantitatively. All of these showed good activity against DPPH radical while 32 of these isolates demonstrated reducing potential also. In addition, some were equally good as ascorbic acid. The present study demonstrated potential of soil fungi to have antioxidant activity similar to plants and mushrooms. High phenolic content of fungi further highlight their significance as new sources of natural antioxidants. These fungi may provide easier set up for production and purification of natural antioxidants as compared to higher plants.


Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5853
Author(s):  
Tuong Thi Le ◽  
Xavier Framboisier ◽  
Arnaud Aymes ◽  
Armelle Ropars ◽  
Jean-Pol Frippiat ◽  
...  

In this study, phenolic compounds from an aqueous protein by-product from rapeseed meal (RSM) were identified by HPLC-DAD and HPLC-ESI-MS, including sinapine, sinapic acid, sinapoyl glucose, and 1,2-di-sinapoyl gentibiose. The main phenolic compound in this by-product was sinapine. We also performed acid hydrolysis to convert sinapine, and sinapic acid derivatives present in the permeate, to sinapic acid. The adsorption of phenolic compounds was investigated using five macroporous resins, including XAD4, XAD7, XAD16, XAD1180, and HP20. Among them, XAD16 showed the highest total phenolic contents adsorption capacities. The adsorption behavior of phenolic compounds was described by pseudo-second-order and Langmuir models. Moreover, thermodynamics tests demonstrated that the adsorption process of phenolic compounds was exothermic and spontaneous. The highest desorption ratio was obtained with 30% (v/v) and 70% (v/v) ethanol for sinapine and sinapic acid, respectively, with a desorption ratio of 63.19 ± 0.03% and 94.68 ± 0.013%. DPPH and ABTS tests revealed that the antioxidant activity of the hydrolyzed fraction was higher than the non-hydrolyzed fraction and higher than the one of vitamin C. Antioxidant tests demonstrated that these phenolic compounds could be used as natural antioxidants, which can be applied in the food industry.


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