Morphological variability and taxonomic concepts of Broad-leaved Helleborine ingroup Epipactis helleborine (L.) Crantz / Raznolikost morfoloških lastnosti in taksonomski koncepti oblikovnega kroga širokolistne močvirnice Epipactis helleborine (L.) Crantz

2020 ◽  
Vol 61 (2) ◽  
pp. 97-125
Author(s):  
Andreja Urbanek Krajnc ◽  
Anja Ivanuš ◽  
Zlata Luthar ◽  
Matej Lipovšek
Keyword(s):  
Molecular Markers ◽  
Genetic Relationships ◽  
Morphological Variability ◽  
Morphological Diversity ◽  
Review Article ◽  
Research Groups ◽  
Species Status ◽  
Phylogenetic Level ◽  
Molecular Phylogenetic ◽  
Epipactis Helleborine

The Broad-leaved Helleborine (Epipactis helleborine s.l.) represents a taxonomically complex group with wide morphological diversity and close genetic relationships among the Epipactis taxa. The transition of some taxa to self-pollination leads to initial speciation from the paraphyletic background of the Broad-leaved Helleborine. In the last twenty years, several new taxa have been awarded species status. However, these taxa are morphologically poorly defined, the descriptions are often contradictory and they actually merit the rank of a subspecies, variety or a form. The taxonomic issue is being explored by several research groups at molecular phylogenetic level. Their results are difficult to integrate into a precise phylogenetic tree, as researchers most often address few local taxa using a variety of more or less supportive molecular markers. In the present review article, the use of an appropriate combination of morphological and molecular markers has been emphasized as being crucial in resolving taxonomic relationships within the Broad-leaved Helleborine. Key words: Epipactis helleborine s.l., Broad-leaved Helleborine, taxonomic problems, speciation   Izvleček Oblikovni krog širokolistne močvirnice (Epipactis helleborine s.l.) predstavlja taksonomsko zapleteno skupino s široko morfološko raznolikostjo in ožjo genetsko sorodnostjo taksonov. Na težavnost določanja nekaterih taksonov vpliva prehod k samoopraševanju, ki vodi k začetni speciaciji iz parafiletskega ozadja širokolistne močvirnice. V zadnjih dvajsetih letih je bilo opisanih več novih taksonov, vendar so ti morfološko slabo opredeljeni, opisi so pogosto nasprotujoči. Taksonomski nivo vrste je večinoma neupravičen in bi jih morali umestiti na nivo podvrste ali različice. Filogenetsko problematiko znotraj oblikovnega kroga širokolistne močvirnice proučuje nekaj raziskovalnih skupin. Njihove rezultate je težko povezati v jasno filogenetsko drevo, saj raziskovalci največkrat obravnavajo nekaj lokalnih taksonov, pri čemer uporabljajo različne, bolj ali manj podporne molekulske metode. V preglednem članku izpostavljamo, da je pri reševanju taksonomskih odnosov ključna uporaba ustrezne kombinacije tako morfoloških kot tudi molekulskih markerjev. Ključne besede: Epipactis helleborine s.l., širokolistna močvirnica, taksonomska problematika, speciacija

scholarly journals Black spicules from a new interstitial opheliid polychaete Thoracophelia minuta sp. nov. (Annelida: Opheliidae)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Naoto Jimi ◽  
Shinta Fujimoto ◽  
Mami Takehara ◽  
Satoshi Imura
Keyword(s):  
Developmental Biology ◽  
Phylogenetic Analysis ◽  
New Species ◽  
Morphological Diversity ◽  
The Body ◽  
Evolutionary Significance ◽  
Research Subject ◽  
Ecological Diversity ◽  
Coelomic Cavity ◽  
Molecular Phylogenetic

AbstractThe phylum Annelida exhibits high morphological diversity coupled with its extensive ecological diversity, and the process of its evolution has been an attractive research subject for many researchers. Its representatives are also extensively studied in fields of ecology and developmental biology and important in many other biology related disciplines. The study of biomineralisation is one of them. Some annelid groups are well known to form calcified tubes but other forms of biomineralisation are also known. Herein, we report a new interstitial annelid species with black spicules, Thoracophelia minuta sp. nov., from Yoichi, Hokkaido, Japan. Spicules are minute calcium carbonate inclusions found across the body and in this new species, numerous black rod-like inclusions of calcium-rich composition are distributed in the coelomic cavity. The new species can be distinguished from other known species of the genus by these conspicuous spicules, shape of branchiae and body formula. Further, the new species’ body size is apparently smaller than its congeners. Based on our molecular phylogenetic analysis using 18S and 28S sequences, we discuss the evolutionary significance of the new species’ spicules and also the species' progenetic origin.

scholarly journals The Role of Botanical Families in Medicinal Ethnobotany: A Phylogenetic Perspective

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 163
Author(s):  
Airy Gras ◽  
Oriane Hidalgo ◽  
Ugo D’Ambrosio ◽  
Montse Parada ◽  
Teresa Garnatje ◽  
...  
Keyword(s):  
Diversity Indices ◽  
Specific Level ◽  
Medicinal Uses ◽  
The Family ◽  
Family Level ◽  
Phylogenetic Level ◽  
Molecular Phylogenetic ◽  
Quantitative Analyses ◽  
Medicinal Flora

Studies suggesting that medicinal plants are not chosen at random are becoming more common. The goal of this work is to shed light on the role of botanical families in ethnobotany, depicting in a molecular phylogenetic frame the relationships between families and medicinal uses of vascular plants in several Catalan-speaking territories. The simple quantitative analyses for ailments categories and the construction of families and disorders matrix were carried out in this study. A Bayesian approach was used to estimate the over- and underused families in the medicinal flora. Phylogenetically informed analyses were carried out to identify lineages in which there is an overrepresentation of families in a given category of use, i.e., hot nodes. The ethnobotanicity index, at a specific level, was calculated and also adapted to the family level. Two diversity indices to measure the richness of reported taxa within each family were calculated. A total of 47,630 use reports were analysed. These uses are grouped in 120 botanical families. The ethnobotanicity index for this area is 14.44% and the ethnobotanicity index at the family level is 68.21%. The most-reported families are Lamiaceae and Asteraceae and the most reported troubles are disorders of the digestive and nutritional system. Based on the meta-analytic results, indicating hot nodes of useful plants at the phylogenetic level, specific ethnopharmacological research may be suggested, including a phytochemical approach of particularly interesting taxa.

Species delimitation and population structure in three Onosma (Boraginaceae) species

Biologia ◽  
2015 ◽  
Vol 70 (10) ◽  
Author(s):  
Masoud Sheidai ◽  
Samira Sadeghi ◽  
Mahnaz-Arab Ameri ◽  
Ahmad-Reza Mehrabeian
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Species Delimitation ◽  
Genetic Difference ◽  
Morphological Variability ◽  
Morphological Diversity ◽  
Mantel Test ◽  
Geographical Distance ◽  
Nj Tree ◽  
Issr Molecular Markers

AbstractThe present study aimed to reveal genetic diversity and population structure in three Onosma species i.e., Onosma bulbotrichum DC., O. elwendicum Wettst. and O. sericeum Willd. (Boraginaceae) growing in Iran. This study was performed to check if the ISSR molecular markers could be used in the Onosma species delimitation. We also investigated the infra-specific morphological variability and if we can identify infra-species taxonomic forms. The present study revealed within species genetic and morphological diversity. ISSR data could delimit the studied species as they were separated from each other in NJ tree, maximum parsimony and STRUCTURE analysis. AMOVA and Hickory tests produced significant genetic difference among the studied species. The Mantel test showed no correlation between genetic distance and geographical distance of the populations for all three species. Reticulation analysis and Nm estimation showed gene flow among these populations. We could identify a few ISSR loci that are adaptive. Data obtained can be used in conservation of these medicinal plants in Iran.

scholarly journals In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

2017 ◽  
Vol 45 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Constantinos SALIS ◽  
Ioannis E. PAPADAKIS ◽  
Spyridon KINTZIOS ◽  
Marianna HAGIDIMITRIOU
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Root Length ◽  
In Vitro Propagation ◽  
Genetic Relationships ◽  
Shoot Proliferation ◽  
Poncirus Trifoliata ◽  
Naphthaleneacetic Acid ◽  
Issr Molecular Markers

The behavior of six citrus rootstocks, Volkameriana, Citrumelo ‘Swingle’, Citrange ‘Carrizo’, Poncirus trifoliata ‘Serra’, Poncirus trifoliata ‘Rubidoux’ and Poncirus trifoliata ‘Flying Dragon’, in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock ‘Swingle’ showed a higher number of roots per explant (4.2) followed by ‘Flying Dragon’ (3.93) and ‘Carrizo’ (3.23) rootstocks. The rootstocks ‘Swingle’ (140.8 mm), Volkameriana (148 mm) and ‘Flying Dragon’ (131.12 mm) had significantly higher root length per explant compared to ‘Carrizo’ (31 mm) and ‘Rubidoux’ (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata ‘Serra’ and Citrumelo ‘Swingle’) and 0.60 (Volkameriana and Citrumelo ‘Swingle’). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

scholarly journals Assessment of genetic diversity in Indian Barnyard millet (Echinochloa spp. complex) using morphological and molecular markers

2016 ◽  
Vol 8 (3) ◽  
pp. 1643-1648 ◽  
Author(s):  
M. P. Moharil ◽  
Dipti Gawai ◽  
N. Dikshit ◽  
M.S. Dudhare ◽  
P. V. Jadhav
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Improvement ◽  
Molecular Diversity ◽  
Genetic Relationships ◽  
Barnyard Millet ◽  
Clear Cut ◽  
Cultivated Species ◽  
High Degree

In the present study, morphological and molecular markers (RAPD primers) were used to analyze the genetic diversity and genetic relationships among 21 accessions of Echinochloa spp. complex comprising the wild and cultivated species collected from Melghat and adjoining regions of Vidarbha, Maharashtra. The availability of diverse genetic resources is a prerequisite for genetic improvement of any crop including barnyard millet. A high degree of molecular diversity among the landraces was detected. Among the 21 genotypes, two major groups (A and B) were formed, at 67.28 % similarity, which clearly encompasses 15 accessions of E. frumentacea and 6 accessions of E. colona. Higher similarity was observed in accessions of E. frumentacea. The accessions IC 597322 and IC 597323 also IC 597302 and IC 597304 showed more than 94% similarity among themselves. The classification of genetic diversity has enabled clear-cut grouping of barnyard millet accessions into two morphological races (E. frumentacea and E. colona).

scholarly journals Hidden tribe: A new species of Stream Toad of the genus Ansonia Stoliczka, 1870 (Anura: Bufonidae) from the poorly explored mountainous borderlands of western Thailand

2021 ◽  
Vol 71 ◽  
pp. 763-779
Author(s):  
Chatmongkon Suwannapoom ◽  
L. Lee Grismer ◽  
Parinya Pawangkhanant ◽  
Mali Naiduangchan ◽  
Platon V. Yushchenko ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Color Pattern ◽  
Sister Species ◽  
Mountain Region ◽  
Unique Combination ◽  
Species Status ◽  
Molecular Phylogenetic ◽  
A New Species

Abstract The integrated results of morphological and molecular phylogenetic analyses confirmed the new species status of a recently discovered population of Ansonia from Suan Phueng District, Ratchaburi Province, Thailand. Ansonia karensp. nov. is separated from all other species of Ansonia by a unique combination of mensural, discrete morphological, and color pattern characteristics and is the sister species of A. thinthinae from Tanintharyi Division, Myanmar. This discovery fills a geographic hiatus of 350 km between it and A. kraensis from Ranong Province, Thailand. Ansonia karensp. nov. is the newest member of a long list of range-restricted endemics having been recently discovered in the northern Tenasserim Mountain region of western Thailand and continues to underscore the unexplored nature of this region and its need for conservation.

Phylogenetics of the mycoheterotrophic genus Thismia (Thismiaceae: Dioscoreales) with a focus on the Old World taxa: delineation of novel natural groups and insights into the evolution of morphological traits

2020 ◽  
Vol 193 (3) ◽  
pp. 287-315 ◽  
Author(s):  
Ekaterina A Shepeleva ◽  
Mikhail I Schelkunov ◽  
Michal Hroneš ◽  
Michal Sochor ◽  
Martin Dančák ◽  
...  
Keyword(s):  
Significant Proportion ◽  
Morphological Diversity ◽  
Taxonomic Revision ◽  
Morphological Characters ◽  
Morphological Description ◽  
Molecular Evidence ◽  
Ancestral State ◽  
Old World ◽  
Molecular Phylogenetic ◽  
Floral Characters

Abstract Thismia is a genus of > 80 mycoheterotrophic species characterized by a peculiar appearance and complex floral morphology. A significant proportion of the species and morphological diversity of Thismia has only been uncovered in the past two decades, and new discoveries continue to be made. Given that many new data have recently become available, and the most comprehensive taxonomic revision of the genus from 1938 addresses less than half of the currently known species, previous hypotheses for species relationships and infrageneric taxonomic classification in Thismia was in need of review. Extensive molecular phylogenetic studies of Thismia at the genus level have never been presented. We investigate the phylogenetic relationships of 41 species (and one variety) of Thismia from the Old World. Our study comprises 68 specimens (for 28 of which the data were newly generated), including outgroup taxa broadly representing Thismiaceae (= Burmanniaceae p.p. sensuAPG IV, 2016), and is based on two nuclear and one mitochondrial marker. We use maximum likelihood and Bayesian inference to infer relationships among the taxa. We also constructed a morphological dataset of 12 mostly floral characters, comparing these characters to hypotheses based on molecular evidence to identify putative synapomorphies for major clades and to discuss hypotheses regarding the evolution of structural traits in the genus. Our analyses indicate that the majority of currently accepted infrageneric taxa of Thismia are polyphyletic. We find support for the monophyly of the Old World group, in which we recognize five well-supported lineages (clades); the only New World species studied appears to be related to the Neotropical genus Tiputinia. Ancestral state reconstructions demonstrate that the evolution of most morphological characters was homoplastic, but we identify characters that provide each of the five clades of Old World Thismia with a unique morphological description. The geographical distribution of the species under study is also shown to be consistent with the major clades. Our investigation provides a phylogenetic basis for the development of a novel sectional classification of Thismia reflecting morphological and geographical traits.

scholarly journals Strategies and Efforts towards the Total Synthesis of Palhinine Alkaloids

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4211
Author(s):  
Yu-Yan Liang ◽  
Shi-Chao Lu ◽  
Ya-Ling Gong ◽  
Shu Xu
Keyword(s):  
Total Synthesis ◽  
Review Article ◽  
Research Groups ◽  
Asymmetric Total Synthesis ◽  
Carbon Cage ◽  
Total Syntheses ◽  
The Past ◽  
Lycopodium Alkaloids

The palhinine family of Lycopodium alkaloids were first reported in 2010, which feature an intriguing isotwistane carbon cage and a nine-membered azonane ring. It is noteworthy that the tetracyclic 5/6/6/9 skeleton was unprecedented in Lycopodium alkaloids before their seminal discovery. Over the past decade, extensive synthetic efforts stemming from seven research groups have resulted in two racemic total syntheses to date. This review article takes the opportunity to survey these efforts and achievements so as to promote further research towards the asymmetric total synthesis of palhinine alkaloids.

scholarly journals A molecular phylogenetic analysis of strombid gastropod morphological diversity

2006 ◽  
Vol 41 (2) ◽  
pp. 436-444 ◽  
Author(s):  
Jared M. Latiolais ◽  
Michael S. Taylor ◽  
Kaustuv Roy ◽  
Michael E. Hellberg
Keyword(s):  
Phylogenetic Analysis ◽  
Morphological Diversity ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Phylogenetic
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