Conidia Shelf-Life and Pathogenicity of Strains of Hirsutella citriformis Speare1 Against Diaphorina citri Kywayama2 Produced on Rice

2019 ◽  
Vol 44 (1) ◽  
pp. 165
Author(s):  
Orquídea Pérez-González ◽  
María Guadalupe Maldonado-Blanco ◽  
Arcadio Valdes-Gonzalez
Keyword(s):  
Shelf Life ◽  
Diaphorina Citri ◽  
Hirsutella Citriformis

scholarly journals Mycelial compatibility, anastomosis, and nucleus numbers of eight Mexican Hirsutella citriformis strains isolated from Diaphorina citri

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11080
Author(s):  
Orquídea Pérez-González ◽  
Ricardo Gomez-Flores ◽  
Patricia Tamez-Guerra
Keyword(s):  
Biocontrol Agent ◽  
Diaphorina Citri ◽  
Potential Biocontrol Agent ◽  
Mycelial Compatibility ◽  
Compatibility Group ◽  
Hirsutella Citriformis ◽  
Safranin O ◽  
Sequence Similarities ◽  
Minimum Medium ◽  
Nuclei Number

Background Among entomopathogenic fungi, H. citriformis has been recognized as potential biocontrol agent against the Asian citrus psyllid Diaphorina citri (Hemiptera: Liviidae). Nevertheless, this fungus is poorly characterized. Previous molecular studies have shown high sequence similarities among strains, but significant differences in Diaphorina citri virulence. Objective The aim of the present study was to determine mycelial compatibility and anastomosis, and nucleus numbers in mycelium and conidia of eight H. citriformis strains isolated from mycosed D. citri adults collected from several Mexican states. Methods Mycelial compatibility and anastomosis evaluation was performed after pairing strains, leading to 36 confrontations, and cultured in chlorate minimum medium to obtain mutants for vegetative compatibility group. Results Hypha or conidia nuclei were visualized with safranin-O and 3% KOH, and 0.05% trypan blue–lactophenol solution. H. citriformis strains showed compatibly and anastomosis events after confrontation. In addition, they showed one nucleus per conidium and mycelium section. It was not possible to obtain H. citriformis nit mutants from the chlorate concentrations tested. Conclusions To date, this is the first report demonstrating mycelial compatibility, anastomosis occurrence, and hyphae and conidia nuclei number among H. citriformis strains.

Observations on the entomopathogenic fungus Hirsutella citriformis attacking adult Diaphorina citri (Hemiptera: Psyllidae) in a managed citrus grove

BioControl ◽  
2012 ◽  
Vol 57 (5) ◽  
pp. 663-675 ◽  
Author(s):  
David G. Hall ◽  
Matthew G. Hentz ◽  
Jason M. Meyer ◽  
Alissa B. Kriss ◽  
Tim R. Gottwald ◽  
...  
Keyword(s):  
Entomopathogenic Fungus ◽  
Diaphorina Citri ◽  
Citrus Grove ◽  
Hirsutella Citriformis

Susceptibility of nymphs and adults of Diaphorina citri to the entomophathogenic fungus Hirsutella citriformis

2017 ◽  
Vol 27 (3) ◽  
pp. 433-438 ◽  
Author(s):  
Karla H. Ibarra-Cortés ◽  
Héctor González-Hernández ◽  
Ariel W. Guzmán-Franco
Keyword(s):  
Diaphorina Citri ◽  
Hirsutella Citriformis

scholarly journals Morphological characterization of Hirsutella citriformis Speare Mexican isolates and evaluation against Diaphorina citri Kuwayama (Hemiptera: Psyllidae)

2014 ◽  
Vol 1 (1) ◽  
Author(s):  
O. Pérez-González ◽  
M. G. Maldonado-Blanco ◽  
R. I. Torres-Acosta ◽  
R. Rodríguez-Guerra ◽  
M. Elías-Santos ◽  
...  
Keyword(s):  
Morphological Characterization ◽  
Diaphorina Citri ◽  
Hirsutella Citriformis

scholarly journals Selektivitas Pestisida Terhadap Perkembangan Cendawan Entomopatogen Hirsutella citriformis

2016 ◽  
Vol 24 (2) ◽  
pp. 162
Author(s):  
Mutia Erti Dwiastuti ◽  
Muhammad Iqbal
Keyword(s):  
Diaphorina Citri ◽  
Hirsutella Citriformis

Pemanfaatan cendawan entomopatogen Hirsutella citriformis dalam mengendalikan hama utama tanaman jeruk Diaphorina citri mempunyai arti penting dalam mendukung konsep pengendalian hama terpadu. Entomopatogen dapat mengendalikan imago Diaphorina citri pada konsentrasi 108 konidia/ml, terutama pada musim penghujan. Aplikasi pestisida (insektisida dan fungisida) di lapang  dilakukan oleh petani diduga berpengaruh terhadap H.citriformis belum pernah diteliti. Tujuan penelitian ini adalah mengetahui pengaruh insektisida dan fungisida terhadap perkembangan H.citriformis secara in vitro dan in vivo. Penelitian dilaksanakan di Laboratorium Terpadu dan Rumah Kasa Balai Penelitian Tanaman Jeruk dan Buah Subtropika, Tlekung, mulai Juni 2008 sampai dengan Juni 2009. Penelitian in vitro, menggunakan rancangan acak lengkap dengan sembilan perlakuan, yaitu insektisida berbahan aktif abamektin, sipermetrin, profenofos, dimetoat serta fungisida berbahan aktif  propinep, mankozeb, benomil, bupirimat serta kontrol, dengan tiga ulangan. Penelitian in vivo,  dilakukan pada benih jeruk menggunakan rancangan acak kelompok dengan tiga perlakuan: satu macam insektisida  dan fungisida  terbaik dari hasil penelitian in vitro yaitu yang tidak menekan pertumbuhan H citriformis dan kontrol, masing-masing dengan lima ulangan.  Parameter pengamatan in vitro meliputi diameter koloni H.citriformis dan persentase tingkat hambatan, sedang pengamatan in vivo di rumah kasa  meliputi periode inkubasi pada D.citri dan jumlah imago terinfeksi yang mati. Hasil penelitian laboratorium menunjukkan bahwa  H. citriformis dapat tumbuh cukup optimal pada media buatan yang dicampur dengan insektisida abamektin, sipermetrin, dan profenofos serta fungisida berbahan aktif propinep dan mankozeb. Hasil penelitian di rumah kasa menunjukkan bahwa insektisida profenofos lebih kompatibel disemprot bersama-sama dengan H.citriformis dibanding fungisida bupirimat dalam mengendalikan serangga imago D.citri

A Man's Shelf Life

2007 ◽  
Author(s):  
Darren Braun
Keyword(s):  
Shelf Life

Gum Ghatti Coating Enriched with Clove Oil extends the post Harvest Shelf Life of Banana (Musa acuminata., cv. Robusta) Fruit Stored at Ambient Condition

2018 ◽  
Vol 34 (2) ◽  
pp. 173-183 ◽  
Author(s):  
Arpit V. Joshi ◽  
◽  
Nilanjana S. Baraiya ◽  
Pinal B. Vyas ◽  
T. V. Ramana Rao ◽  
...  
Keyword(s):  
Shelf Life ◽  
Musa Acuminata ◽  
Ambient Condition ◽  
Clove Oil ◽  
Post Harvest ◽  
Gum Ghatti

EFFECT OF LEMON PEEL AND PULP EXTRACT ON SOME LOCAL KURDISH RAM MEAT QUALITIES AND ITS SHELF LIFE

2020 ◽  
pp. 140-153
Keyword(s):  
Shelf Life ◽  
Chemical Properties ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Meat Sample ◽  
Refrigerated Storage ◽  
Immersion Method ◽  
Quality Properties ◽  
Lemon Peel ◽  
Meat Samples

To investigate the effectiveness of adding lemon peels and pulp extracts on some quality properties and shelf-life of the sheep longisimussdorsi muscle during refrigerated storage at 4±1°C for 0, 4, and 8 days for this purpose am meat trim of visible fat and connective tissue, they cut in small cubes. The meat samples divide into four equal proportions and mix with different concentrations of lemon peel and pulp extract according to the following formulations: Control; T1 1%; T2 2% and T3 3% of lemon peel and pulp extract, by applied immersion method. The results showed acceptable results of moisture content, Water-holding capacity cooking loss, thiobarbituric acid, met-myoglobin, myoglobin, and sensory traits of the samples treated with lemon peel in comparison to the control group. The phiso-chemical traits changed during the storage periods but the meat sample treated with lemon extract was more stable than control groups. These results suggested that using lemon peels and pulp extracts to maintain physio-chemical properties of ram meat and extend shelf-life during refrigerated storage, which may have implications of meat processors.

Thysanoptera diversity associated with Mexican lemon (Citrus aurantifolia Christm.)

2020 ◽  
Vol 13 (9) ◽  
Author(s):  
Francisco Javier Avendaño-Gutiérrez ◽  
Salvador Aguirre Paleo ◽  
Alejandro Morales Hernández ◽  
Venecia Quesadas-Béjar
Keyword(s):  
Biosphere Reserve ◽  
Simple Random Sampling ◽  
Diversity Indices ◽  
Diaphorina Citri ◽  
Program Calculation ◽  
Homogeneous Relation ◽  
Candidatus Liberibacter ◽  
Scirtothrips Citri ◽  
Species Richness And Abundance ◽  
First Time

Objective: To calculate the monthly relative abundance of Thysanoptera species, according to the Margalef, Simpson and Shannon-Wiener diversity indices. Design / Methodology / Approach: The work was carried out in three geographic units with conventional management, during January-December, 2019 in the Reserva de la Biosfera Zicuirán-Infiernillo (Biosphere Reserve), Michoacán, Mexico. In each unit, 10 trees were selected through simple random sampling. Thrips counts were performed on ten shoots per tree every 15 d, for a total of 7200 shoots in the three geographic units. Thysanoptera individuals were placed in entomological jars. The variables were: number of thrips collected per shoot in sampled tree and geographic unit (orchard). To estimate the specific richness and structure of species, the program "calculation of diversity indices DIVERS" was used. Results: In the three geographical units studied, the recorded presence of Thysanoptera accounted for 12 to 17 species. For Nueva Italia 12 recorded species, two were permanent (16.66%), five abundant (41.66%), one scarce (8.3%) and four rare (33.33%). In Zicuirán, three species were permanent (17.64%), six abundant (35.29%), two scarce (11.76%) and six rare (35.29%). In Los Hoyos, four species were permanent (26.66%), four abundant (26.66%) and seven rare (46.66%). The abundance of species was represented by the genus Frankliniella and the species Scolothrips sexmaculatus and Scirtothrips citri. The highest species richness and abundance was found from January to May. In October and November, the value of the calculated indices was zero, which shows less richness and abundance of individuals. The best species uniformity was recorded during January and December, which meant a more stable and homogeneous relation. Study limitations/Implications. Pest resurgence, presence of Candidatus Liberibacter spp. and its vector Diaphorina citri. Findings / Conclusions: in Nueva Italia, 12 species were taxonomically determined; in Los Hoyos 15, and in Zicuirán 17 species, which are reported for the first time in the state of Michoacán, Mexico. At the geographic unit "Los Hoyos" diversity was higher, uniform and stable.

PENGARUH VIRGIN COCONUT OIL (VCO) TERHADAP KARAKTERISTIK DAN UMUR SIMPAN ROTI MANIS

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Aulia Alfi
Keyword(s):  
Moisture Content ◽  
Shelf Life ◽  
Coconut Oil ◽  
Temperature Treatment ◽  
Ash Content ◽  
Virgin Coconut Oil ◽  
Food Ingredients ◽  
Life Analysis ◽  
Antibacterial And Antifungal ◽  
Physical And Chemical

Virgin Coconut Oil (VCO) adalah bahan alami yang memiliki sifat antimikroba (antivirus, antibakteri, dan antijamur). Sehingga VCO dapat memberikan efek pengawet pada bahan makanan, salah satunya adalah roti manis. Penelitian ini dilakukan untuk mengevaluasi pengaruh VCO terhadap karakteristik (fisik dan kimia) dan umur simpan roti manis. Roti manis dianalisis secara fisik (tekstur dan porositas) dan kimia (kadar air, kadar abu, kadar lemak, kadar protein, dan kandungan karbohidrat), dan analisis umur simpan dengan FFA, uji organoleptik dan jamur setiap dua hari selama delapan hari penyimpanan di suhu ruang. Variasi perlakuan roti manis adalah dari rasio konsentrasi VCO: margarin: mentega, K (0%: 8%: 8%); A (4%: 6%: 6%); B (8%: 4%: 4%), C (12%: 2%: 2%); D (16%: 0%: 0%). Hasil penelitian menunjukkan bahwa VCO tidak memiliki pengaruh yang signifikan terhadap karakteristik fisik dan karakteristik kimia roti manis. Namun, VCO berpengaruh signifikan terhadap kadar air roti manis yang dihasilkan, roti manis K memiliki kadar air tertinggi (22,36%) dan berbeda dengan sampel roti manis lainnya. VCO secara efektif menghambat pertumbuhan jamur di roti manis pada konsentrasi 8%, 12%, dan 16%. Roti manis K dan A memiliki masa simpan 4 hari, sedangkan roti manis B, C, dan D memiliki masa simpan 6 hari.Kata kunci: VCO, roti manis, karakteristik, umur simpanABSTRACTVirgin Coconut Oil (VCO) is a natural ingredient that has antimicrobial (antiviral, antibacterial, and antifungal) properties. So that VCO can provide a preservative effect on food ingredients, one of which is sweet bread. This research was conducted to evaluate the effect of VCO on characteristics (physical and chemical) and shelf life of sweet bread. Sweet bread was analyzed physically (texture and porosity) and chemistry (moisture content, ash content, fat content, protein content, and carbohydrate content), and shelf life analysis with FFA, organoleptic and mold tests every two days for eight days of storage at ambient temperature. Treatment variations of sweet breads is from the ratio of the concentration of VCO: margarine: butter, K (0%: 8%: 8%); A (4%: 6%: 6%); B (8%: 4%: 4%), C (12%: 2%: 2%); D (16%: 0%: 0%). The results showed that VCO did not have a significant effect on the physical characteristics and chemical characteristics of sweet bread. However, the VCO has a significant effect on the water content of the sweet bread produced, sweet bread K has the highest moisture content (22,36%) and it is different from other sweet bread samples. VCO effectively inhibits the growth of sweet bread mold at concentrations of 8%, 12%, and 16%. K and A sweet bread has a shelf life of 4 days, while sweet breads B, C, and D have a shelf life of 6 days.Keywords: VCO, sweet bread, characteristics, shelf life

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