scholarly journals Indicator Amino Acid–Derived Estimate of Dietary Protein Requirement for Male Bodybuilders on a Nontraining Day Is Several-Fold Greater than the Current Recommended Dietary Allowance

2017 ◽  
Vol 147 (5) ◽  
pp. 850-857 ◽  
Author(s):  
Arash Bandegan ◽  
Glenda Courtney-Martin ◽  
Mahroukh Rafii ◽  
Paul B Pencharz ◽  
Peter WR Lemon
2019 ◽  
Vol 316 (5) ◽  
pp. E741-E748 ◽  
Author(s):  
Arash Bandegan ◽  
Glenda Courtney-Martin ◽  
Mahroukh Rafii ◽  
Paul B. Pencharz ◽  
Peter W. R. Lemon

Despite studies indicating increased protein requirements in strength-trained or endurance-trained (ET) individuals, the Institute of Medicine has concluded that “no additional dietary protein is suggested for healthy adults undertaking resistance or endurance exercise,” and the controversy regarding exercise effects on protein requirements continues. The objective of this study was to determine the dietary protein requirement of healthy young ET men (≥1 yr training experience) 24 h post exercise (to minimize any acute effects of the previous training session) by measuring the oxidation of ingested l-[1-13C]phenylalanine to 13CO2 in response to graded intakes of protein (indicator amino acid oxidation technique). Eight men [maximal oxygen consumption 64.1 ml·kg−1·min−1 (SD 3.7)] were each studied 24 h postexercise repeatedly with protein intakes ranging from 0.3 to 3.5 g·kg−1·day−1. Protein was fed as an amino acid mixture based on the protein pattern in egg, except for phenylalanine and tyrosine, which were maintained at constant amounts across all protein intakes. For 2 days before the study day, all participants consumed 1.6 g protein·kg−1·day−1. The estimated average requirement (EAR) for protein was determined by applying a nonlinear mixed-effects change-point regression analysis to F13CO2 (label tracer oxidation in 13CO2 breath), which identified a breakpoint in the F13CO2 in response to the graded amounts of protein. The EAR for protein and the upper 95% confidence interval were 2.1 and 2.6 g·kg−1·day−1, respectively. These data suggest that the protein EAR for ET men 24 h postexercise exceeds the Institute of Medicine EAR and established athlete guidelines by ~3.5- and 1.3-fold, respectively.


2015 ◽  
Vol 146 (4) ◽  
pp. 681-687 ◽  
Author(s):  
Mahroukh Rafii ◽  
Karen Chapman ◽  
Rajavel Elango ◽  
Wayne W Campbell ◽  
Ronald O Ball ◽  
...  

2014 ◽  
Vol 145 (1) ◽  
pp. 18-24 ◽  
Author(s):  
Mahroukh Rafii ◽  
Karen Chapman ◽  
Jillian Owens ◽  
Rajavel Elango ◽  
Wayne W Campbell ◽  
...  

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Glenda Courtney‐Martin ◽  
Mahroukh Rafii ◽  
Karen Chapman ◽  
Rajavel Elango ◽  
Wayne W Campbell ◽  
...  

1993 ◽  
Vol 71 (10) ◽  
pp. 2111-2120 ◽  
Author(s):  
Mary E. Murphy

Assumptions about potential mismatches between protein demand and protein availability are implicit in many hypotheses pertaining to the ecology of wild birds. However, few direct measures of the protein requirements of wild birds have been made. I evaluated the requirement for protein for maintenance of a wild granivorous passerine, the White-crowned Sparrow (Zonotrichia leucophrys gambelii). I estimated protein requirement from measures of endogenous nitrogen (N) loss, N balance, and changes in body mass in wintering adult birds. The experimental birds were kept indoors at 23 °C (8.5 h light: 15.5 h dark) and fed semisynthetic diets that differed only in their concentrations of protein (0–17.3%) and starch, which were reciprocally adjusted. The amino acid profiles of the protein in the diets were identical and were formulated to match the average amino acid profile of the mixed proteins of a diet containing grains, seeds, insects, and fruits. In White-crowned Sparrows, the protein requirements for maintenance of body mass (366 mg/d) and a positive N balance equal to that of birds ingesting the acclimation diets (436 mg/d) were 7.3 and 8.7% protein, respectively, in a diet containing 12.4 kJ apparent metabolizable energy per gram dry mass. The estimated minimum maintenance requirement for protein for N equilibrium, derived from regressing N excretion on N intake, equalled 264 mg protein/d, or ca. 5.3% dietary protein. Measures of endogenous N loss (endogenous urinary N loss plus metabolic fecal N loss) equalled 14.7 mg N/d, indicating that White-crowned Sparrows need to replace at least 92 mg body protein/d. Dietary protein content had little influence on the birds' utilization of dietary energy and daily metabolized energy. The apparent contradiction in the requirement for a positive N balance to maintain body mass is discussed.


2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Mahroukh Rafii ◽  
Glenda Courtney‐Martin ◽  
Karen Chapman ◽  
Jillian Owens ◽  
Rajavel Elango ◽  
...  

2009 ◽  
Vol 33 (4) ◽  
pp. 674-681 ◽  
Author(s):  
Xiao-Zhi LIN ◽  
Xiao-Jun XIE ◽  
Yi-Ping LUO

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