scholarly journals Super-resolution Fluorescence Microscopy Based on Single-molecule Localization

2013 ◽  
Vol 22 (11) ◽  
pp. 34
Author(s):  
Jinwoo LEE ◽  
Sungchul HOHNG
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Super Resolution

scholarly journals Super-resolution Localization and Defocused Fluorescence Microscopy on Resonantly Coupled Single-Molecule, Single-Nanorod Hybrids

ACS Nano ◽  
2016 ◽  
Vol 10 (2) ◽  
pp. 2455-2466 ◽  
Author(s):  
Liang Su ◽  
Haifeng Yuan ◽  
Gang Lu ◽  
Susana Rocha ◽  
Michel Orrit ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Super Resolution

scholarly journals Resolving the internal morphology of core–shell microgels with super-resolution fluorescence microscopy

2020 ◽  
Vol 2 (1) ◽  
pp. 323-331 ◽  
Author(s):  
Pia Otto ◽  
Stephan Bergmann ◽  
Alice Sandmeyer ◽  
Maxim Dirksen ◽  
Oliver Wrede ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Internal Structure ◽  
Single Molecule ◽  
Fluorescent Dyes ◽  
Super Resolution ◽  
Core Shell ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Internal Morphology

We investigate the internal structure of smart core–shell microgels by super-resolution fluorescence microscopy by combining of 3D single molecule localization and structured illumination microscopy using freely diffusing fluorescent dyes.

scholarly journals Polarized Resolved Single-Molecule Localization-Based Super-Resolution Fluorescence Microscopy Reveals Orientation Order in Bio-Molecular Assemblies

2014 ◽  
Vol 106 (2) ◽  
pp. 203a-204a
Author(s):  
Haitham Ahmed Shaban ◽  
Cesar A. Valades-Cruz ◽  
Julien Savatier ◽  
Serge Monneret ◽  
Herve Rigneault ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Super Resolution ◽  
Molecular Assemblies ◽  
Orientation Order

scholarly journals 3D Single-Molecule Super-Resolution Fluorescence Microscopy with the Corkscrew Point Spread Function

2016 ◽  
Vol 110 (3) ◽  
pp. 176a
Author(s):  
Maurice Lee ◽  
Matthew Lew ◽  
Alex von Diezmann ◽  
Lucien Weiss ◽  
Yoav Shechtman ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Point Spread Function ◽  
Super Resolution ◽  
Point Spread ◽  
Spread Function

scholarly journals SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Yunqing Tang ◽  
Luru Dai ◽  
Xiaoming Zhang ◽  
Junbai Li ◽  
Johnny Hendriks ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Real Time ◽  
Single Molecule ◽  
Super Resolution ◽  
Localization Algorithm

scholarly journals Developing super-resolution fluorescent microscopy for virology research

2021 ◽  
Vol 63 (11) ◽  
pp. 6-12
Author(s):  
Trong Nghia Nguyen ◽  
◽  
Thi Bich Ngoc Nguyen ◽  
Hong Nhung Tran ◽  
Duc Toan Nguyen ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Dengue Virus ◽  
Single Molecule ◽  
Fluorescent Microscopy ◽  
Super Resolution ◽  
Hemorrhagic Fever ◽  
Reconstruction Process ◽  
Virus Particles ◽  
Microscopy Technique ◽  
Localisation Microscopy

Taking advantage of the use of photoswitchable probes and high precision localisation of single molecules to surpass the diffraction limit, super-resolution fluorescence microscopy allows observing non-invasive live-cell at sub-diffraction size (<200 nm). Given the advantage of super-resolution fluorescence microscopy, our group has reconstructed the super-resolution fluorescence microscopybased on the single-molecule localisation microscopy technique with a resolution of 20 nm. In this research, the authors present the reconstruction process of the microscopy system and its application in observing hemorrhagic fever Dengue virus. Dengue virus was cultured in baby hamster kidney (BHK-21) cells and was then negative stained for transmission electron microscope (TEM) or immunofluorescent labeled for stochastic optical reconstruction microscopy (STORM). The diameter of the Dengue virus particles is 45-60 nm measured using TEM and is 84±12 nm measured using STORM. After subtraction of the length of the antibody attached to the virus particles, the diameter of Dengue virus particles measured using STORM are close to which measured using TEM. In conclusion, the authors highlight the findings of super-resolution fluorescence microscopy-based Dengue virus studies and their contributions to the understanding of Dengue virus particles. The current advances in super-resolution microscopy may open new avenues for future virology teaching and research.

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