scholarly journals Single-molecule Fluorescence Live-cell Imaging

2013 ◽  
Vol 22 (11) ◽  
pp. 28
Author(s):  
Sora YANG ◽  
Dong-Kyun KIM ◽  
Nam Ki LEE
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Single Molecule Fluorescence ◽  
Fluorescence Live Cell Imaging

scholarly journals Autofluorescent Proteins in Single-Molecule Research: Applications to Live Cell Imaging Microscopy

2001 ◽  
Vol 80 (5) ◽  
pp. 2396-2408 ◽  
Author(s):  
Gregory S. Harms ◽  
Laurent Cognet ◽  
Piet H.M. Lommerse ◽  
Gerhard A. Blab ◽  
Thomas Schmidt
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals “Probe, Sample, and Instrument (PSI)”: The Hat-Trick for Fluorescence Live Cell Imaging

Chemosensors ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 40 ◽  
Author(s):  
Ludovic Galas ◽  
Thibault Gallavardin ◽  
Magalie Bénard ◽  
Arnaud Lehner ◽  
Damien Schapman ◽  
...  
Keyword(s):  
Organic Synthesis ◽  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cell Labeling ◽  
Key Points ◽  
Research Infrastructures ◽  
New Strategies ◽  
Fluorescence Live Cell Imaging

Cell Imaging Platforms (CIPs) are research infrastructures offering support to a number of scientific projects including the choice of adapted fluorescent probes for live cell imaging. What to detect in what type of sample and for how long is a major issue with fluorescent probes and, for this, the “hat-trick” “Probe–Sample–Instrument” (PSI) has to be considered. We propose here to deal with key points usually discussed in CIPs including the properties of fluorescent organic probes, the modality of cell labeling, and the best equipment to obtain appropriate spectral, spatial, and temporal resolution. New strategies in organic synthesis and click chemistry for accessing probes with enhanced photophysical characteristics and targeting abilities will also be addressed. Finally, methods for image processing will be described to optimize exploitation of fluorescence signals.

scholarly journals tdLanYFP, a yellow, bright, photostable and pH insensitive fluorescent protein for live cell imaging and FRET-based sensing strategies

2021 ◽  
Author(s):  
Y. Bousmah ◽  
H. Valenta ◽  
G. Bertolin ◽  
U. Singh ◽  
V. Nicolas ◽  
...  
Keyword(s):  
Single Molecule ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Yellow Fluorescent Protein ◽  
Resonance Energy ◽  
Live Cell ◽  
Live Cells

AbstractYellow fluorescent proteins (YFP) are widely used as optical reporters in Förster Resonance Energy Transfer (FRET) based biosensors. Although great improvements have been done, the sensitivity of the biosensors is still limited by the low photostability and the poor fluorescence performances of YFPs at acidic pHs. In fact, today, there is no yellow variant derived from the EYFP with a pK1/2 below ∼5.5. Here, we characterize a new yellow fluorescent protein, tdLanYFP, derived from the tetrameric protein from the cephalochordate B. lanceolatum, LanYFP. With a quantum yield of 0.92 and an extinction coefficient of 133 000 mol−1.L.cm−1, it is, to our knowledge, the brightest dimeric fluorescent protein available, and brighter than most of the monomeric YFPs. Contrasting with EYFP and its derivatives, tdLanYFP has a very high photostability in vitro and preserves this property in live cells. As a consequence, tdLanYFP allows the imaging of cellular structures with sub-diffraction resolution with STED nanoscopy. We also demonstrate that the combination of high brightness and strong photostability is compatible with the use of spectro-microscopies in single molecule regimes. Its very low pK1/2 of 3.9 makes tdLanYFP an excellent tag even at acidic pHs. Finally, we show that tdLanYFP can be a FRET partner either as donor or acceptor in different biosensing modalities. Altogether, these assets make tdLanYFPa very attractive yellow fluorescent protein for long-term or single-molecule live-cell imaging that is also suitable for FRET experiment including at acidic pH.

scholarly journals TADF Dye-Loaded Nanoparticles for Fluorescence Live-Cell Imaging

2020 ◽  
Vol 8 ◽  
Author(s):  
Carina I. C. Crucho ◽  
João Avó ◽  
Ana M. Diniz ◽  
Sandra N. Pinto ◽  
José Barbosa ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Live Cell Imaging

Analysis of the influence of subcellular localization of the HIV Rev protein on Rev-dependent gene expression by multi-fluorescence live-cell imaging

2006 ◽  
Vol 312 (4) ◽  
pp. 443-456 ◽  
Author(s):  
Horst Wolff ◽  
Kamyar Hadian ◽  
Manja Ziegler ◽  
Claudia Weierich ◽  
Susanne Kramer-Hammerle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Subcellular Localization ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Rev Protein ◽  
Fluorescence Live Cell Imaging

Concentration-dependent fluorescence live-cell imaging and tracking of intracellular nanoparticles

2011 ◽  
Vol 22 (23) ◽  
pp. 235101 ◽  
Author(s):  
Ji Hye Seo ◽  
Keunchang Cho ◽  
So Yeong Lee ◽  
Sang-Woo Joo
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Live Cell Imaging
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