scholarly journals Characterization of Vibrio spp. in environmental water samples collected from flood prone areas of Bangladesh and their antibiotic resistance profile

2021 ◽  
Vol 7 (4) ◽  
pp. 471-480
Author(s):  
Md. Aoulad Hosen ◽  
◽  
Fozol Korim Ovi ◽  
Harunur Rashid ◽  
MD. Hasibul Hasan ◽  
...  
Keyword(s):  
Water Samples ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Pond Water ◽  
Environmental Water ◽  
Well Water ◽  
Isolation And Identification ◽  
Alkaline Peptone Water ◽  
Antibiotic Resistance Profile ◽  
Tube Well

<abstract> <p>Last cholera epidemic has been recorded in Bangladesh between 1992–1993, while few sporadic localized outbreaks have been reported as recent as 2005. Serotype O1 of <italic>Vibrio cholera</italic> is considered as the principal causative agent which transmits through contaminated drinking water resulting that epidemic. Therefore, the objective of this research was to isolate <italic>V. cholera</italic> in 3 different water sources; River, pond and tube-well, in 5 different locations of Gazipur, Bangladesh, and to analyze their antibiogram study. A total of 45 water samples were randomly collected for the isolation and identification of <italic>Vibrio</italic> spp. Samples are then serially diluted in alkaline peptone water and streak on Thiosulfate Citrate Bile Salt Sucrose-TCBS agar for quantification of <italic>V</italic>. spp. For <italic>V. cholera</italic> isolation water samples were first enriched in nutrient broth at 37 °C for 16 hours followed by cultivation in selective media; TCBS agar at 37 °C for 24 hours. Yellow colonies on TCBS agar were screed as <italic>V. cholera</italic> and was confirmed by analyzing their biochemical characteristics like Catalase, Oxidase, MR, VP, Indole, Sugar fermentation. Following isolation antibiotic sensitivity test was performed on each <italic>V. cholera</italic> isolates to determine their antibiotic sensitivity profile. The results showed, out of 45 samples 12 contained <italic>V. cholera</italic>. Tube-well water has significantly lower concentration (log CFU/mL) of <italic>V</italic>. spp. than river and pond water (P &lt; 0.05). Bacterial concentration doesn't deviate (P &gt; 0.05) significantly in 5 different location the sample was collected from. All the 12 isolates were sensitive to Gentamicin and ciprofloxacin (100%), while Chloramphenicol (91.67%), Sulfamethoxazole (91.67%), Azithromycin (66.67%) showed high sensitivity. Isolates showed marginal sensitivity towards Tetracycline (33.33%), and Cephalexin (16.67%) and 100% resistance against antibiotics like Vancomycin, Penicillin, Erythromycin, and Nalidixic Acid. Based on these data we recommend using tube-well water instead of river and pond water for drinking purposes. Furthermore, we suggest selective use of sensitive antimicrobials listed here for therapeutics of cholera outbreak.</p> </abstract>

Modern nanobiotechnologies for efficient detection and remediation of mercury

Sensor Review ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Mulayam Singh Gaur ◽  
Rajni Yadav ◽  
Mamta Kushwah ◽  
Anna Nikolaevna Berlina
Keyword(s):  
Heavy Metals ◽  
Water Samples ◽  
Low Cost ◽  
High Sensitivity ◽  
Environmental Water ◽  
Mercury Ions ◽  
Content Type ◽  
Efficient Detection ◽  
Sensitivity And Selectivity ◽  
Selection Of

Purpose This information will be useful in the selection of materials and technology for the detection and removal of mercury ions at a low cost and with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. Design/methodology/approach Different nano- and bio-materials allowed for the development of a variety of biosensors – colorimetric, chemiluminescent, electrochemical, whole-cell and aptasensors – are described. The materials used for their development also make it possible to use them in removing heavy metals, which are toxic contaminants, from environmental water samples. Findings This review focuses on different technologies, tools and materials for mercury (heavy metals) detection and remediation to environmental samples. Originality/value This review gives up-to-date and systemic information on modern nanotechnology methods for heavy metal detection. Different recognition molecules and nanomaterials have been discussed for remediation to water samples. The present review may provide valuable information to researchers regarding novel mercury ions detection sensors and encourage them for further research/development.

scholarly journals Fast quantification of fluoroquinolones in environmental water samples using molecularly imprinted polymers coupled with internal extractive electrospray ionization mass spectrometry

RSC Advances ◽  
2018 ◽  
Vol 8 (31) ◽  
pp. 17293-17299 ◽  
Author(s):  
Wei Kou ◽  
Hua Zhang ◽  
Aisha Bibi ◽  
Mufang Ke ◽  
Jing Han ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecularly Imprinted Polymers ◽  
Water Samples ◽  
High Sensitivity ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Ionization Mass ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Sensitivity Method

A simple, fast and high-sensitivity method for quantification of fluoroquinolones in environmental water samples using MIPs-iEESI-MS.

scholarly journals Isolation Of Extended-Spectrum B-Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumiae From Dr. Zainoel Abidin General Hospital, Aceh

2019 ◽  
Vol 4 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Zinatul Hayati ◽  
Syamsul Rizal ◽  
Ridhia Putri
Keyword(s):  
Escherichia Coli ◽  
General Hospital ◽  
Clinical Specimen ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Clinical Microbiology Laboratory ◽  
Isolation And Identification ◽  
E Coli ◽  
Extended Spectrum ◽  
Sensitivity Pattern

Infection that occurs in Indonesia has increased more significantly than before, compared to the increasing bacterial multidrug resistance (MDR) as the cause of infection. A study conducted in 5 hospitals in Indonesia in 2013 showed that the prevalence rate of extended-spectrum β-lactamase (ESBL)-producing bacteria reached 32-68%. The objective of this study is to detect the prevalence and resistence pattern of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Dr. Zainoel Abidin General Hospital, Banda Aceh. This study was conducted from 1 September 2016 to 31 December 2016. Specimen types included in this study were blood, sputum, urine, pus, mucosal swab, and another body fluids sample. The sampling method in this study was total sampling that is all clinical specimen examined in Clinical Microbiology Laboratory. Isolation and identification ESBL-producing bacteria was performed by VITEK-2 machine (Biomerieux). The result of this study is that a total 122 E. coli and K. pneumoniae were isolated. That consisted of 48 (39%) E. coli isolates and 74 (61%) K. pneumoniae isolates. From 48 E. coli isolates it was found out that 41 (85%) had ESBL phenotypes and from 74 K. pneumoniae isolates it was found out that 59 (80%) had ESBL phenotypes. In total, 100 (82%) isolates from 122 isolates had ESBL phenotypes. Distribution of ESBL-producing E. coli and K. pneumoniae based on sample was 24 (89%) isolates from the total of 27 urine isolates, 18 (95%) isolates from the total of 19 blood isolates, 28 (78%) isolates from the total of 36 sputum isolates, and 30 (75%) isolates from the total of 40 pus isolates. Antibiotic sensitivity pattern of the E. coli and K. pneumoniae isolates had high sensitivity to amycasin dan meropenem which was above 89%. Meanwhile, it also had sensitivity to Fosfomycin and Piperacyclin-Tazobactam by 80% and 77% respectively. Another antibiotic was less effective

scholarly journals Determination of Drinking Water Quality: A Case Study on Saline Prone South-West Coastal Belt of Bangladesh

2017 ◽  
Vol 10 (1) ◽  
pp. 101-108
Author(s):  
TK Das ◽  
M Choudhury ◽  
M Sultana
Keyword(s):  
Drinking Water ◽  
Pond Water ◽  
Rain Water ◽  
Well Water ◽  
Sand Filter ◽  
Coastal Belt ◽  
Water Pond ◽  
Filter Water ◽  
Tube Well

This research integrates quantitative data of underground and sub-surface sources of drinking water from two coastal union Gabura and Munshiganj of Shyamnagur upazila of Satkhira district. Four drinking water sources have been selected and sampled during dry season when the salinity rate is generally high. A total of 22 water samples have been collected of which 6 from protected pond, 6 from tube well, 6 from rainwater and 4 from pond sand filter (PSF). The average pH of tube well water, protected pond water, pond sand filter water and rain water were 6.59 (±.05), 6.46 (±1.03), 6.60 (±.52), 5.95 (±.87), respectively. The average EC (μs cm-1) range of tube well water, protected pond water, pond sand filter water and rain water were 2059.16 (±49.43), 1756 (±14), 769 (±94.27), 64.33(±59.91) while the TDS (ppm) range were 1319.97 (±31.69), 1125.64 (±913.46), 492.94 (±60.43) and 15.38 (±118.59) respectively. The maximum concentration of salinity was found in tube well water which was 1.37 (±53) ppt. Most of the protected ponds were highly contaminated with sulphate and it ranges from 84.64 to 218.25 ppm. The average number of fecal Coliform in protected ponds were 1650 100 m l-1 of water while in PSF source pond 2350 100ml-1 and collection point 1.5 100ml-1.J. Environ. Sci. & Natural Resources, 10(1): 101-108 2017

scholarly journals Prevalence and antibiotic resistance profile of Listeria spp. associated with seafoods from fish catchment areas in Kerala, India

2021 ◽  
Vol 14 (3) ◽  
pp. 777-783
Author(s):  
K. Vrinda Menon ◽  
B. Sunil ◽  
C. Latha
Keyword(s):  
Antibiotic Resistance ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Isolation And Identification ◽  
Resistance Profile ◽  
Fresh Fish ◽  
Antibiotic Resistance Profile ◽  
Catchment Areas ◽  
Antibiotic Sensitivity Test ◽  
Serotype 4B

Background and Aim: Listeria monocytogenes is a ubiquitous, intracellular pathogen which has been implicated as a cause of several foodborne outbreaks. This study aimed to generate information on the prevalence and antibiotic resistance profile of Listeria species isolated from seafood. Materials and Methods: A total of 400 samples of fresh fish, 100 samples of dry fish and 200 samples each of crustaceans and mollusks were collected from the fish catchment areas. All the samples were subjected to isolation and identification of Listeria spp. by two-step enrichment in UVM broth and plating on selective agar media (PALCAM) and then subjected to molecular characterization. L. monocytogenes isolates obtained during the study were subjected to serotyping by multiplex polymerase chain reaction. The isolates were also subjected to antibiotic sensitivity test. Results: The prevalence of L. monocytogenes in seafoods in the present study was 0.55%. The isolates of L. monocytogenes were found to possess all virulence genes, namely, iap, hlyA, actA, prfA, plcA, and inlA. All the isolates belonged to serotype 4b. The occurrence of Listeria innocua was found to be more and was detected in 16.77% of seafoods samples. Antibiotic sensitivity test revealed that all isolates were resistant to cefixime but were sensitive to almost all other commonly used antibiotics. Conclusion: The presence of Listeria spp. in raw seafood samples augments the need for implementation of good hygienic practices during the handling and processing of seafoods to safeguard the health of the consumers.

scholarly journals Manganese in potable water of nine districts, Bangladesh: Human health risk

2021 ◽  
Author(s):  
Md. Aminur Rahman ◽  
Md. Abul Hashem ◽  
Md. Sohel Rana ◽  
Md. Rashidul Islam
Keyword(s):  
Drinking Water ◽  
Human Health ◽  
Water Samples ◽  
Potable Water ◽  
Hazard Quotient ◽  
Human Health Risk ◽  
World Health ◽  
Well Water ◽  
Tube Well ◽  
Health Organization

Abstract Safe drinking water is directly linked to good human health. An excessive amount of manganese (Mn) in drinking water supplies causes people show symptoms of neurotoxicity. In this study, the level of Mn in potable water sourced from tube wells located in 9 (nine) districts of Bangladesh was monitored. In total 170 (one hundred and seventy) water samples were collected and Mn was quantified by atomic absorption spectroscopy (AAS). The levels of Mn found in the tube well water samples of Sirajganj, Meherpur, Chuadanga, Jhenaidah, Magura, Faridpur, Jashore, Satkhira, and Khulna were 0.37–1.86, 0.10–4.11, 0.30–0.76, 0.26–0.94, 0.01–0.18, 0.21–1.78, 0.08–1.23, 0.05–0.27 and 0.01–2.11 mg/L, respectively. Results revealed that Mn level was beyond the highest contaminated levels of 0.1 mg/L and 0.4 mg/L, which are recommended by Bangladesh Drinking Standard (BDS) and World Health Organization (WHO), respectively. The maximum Mn contaminated level reached up to 4.11 mg/L (mean: 0.53 mg/L). The Mn level in tube well water exceeded 51.1% and 75.9% set by the recommended value of WHO and BDS, respectively. Furthermore, the calculated hazard quotient (HQ) value for Mn was observed to be greater than unity, indicating both children and adults risked potential non-carcinogenic health issues. The water supply authorities should take steps to provide Mn-free drinking water for communities.

scholarly journals Arsenic levels in tube-wells water, food, residents' urine and the prevalence of skin lesions in Yatenga province, Burkina Faso

2012 ◽  
Vol 5 (1) ◽  
pp. 38-41 ◽  
Author(s):  
Issa Somé ◽  
Abdoul Sakira ◽  
Moustapha Ouédraogo ◽  
Theodore Ouédraogo ◽  
Adama Traoré ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Water Samples ◽  
Arsenic Concentration ◽  
Public Health Problem ◽  
Skin Lesions ◽  
Well Water ◽  
Major Public Health Problem ◽  
Tube Wells ◽  
Guideline Value ◽  
Tube Well

Arsenic levels in tube-wells water, food, residents' urine and the prevalence of skin lesions in Yatenga province, Burkina FasoThe aim of the present study was to evaluate the levels of arsenic in tube-well water, food and residents' urines samples in Yatenga province, Burkina Faso. The prevalence of skin lesions was evaluated as well. The study was cross-sectional in design. It was conducted during April 2009. Permanent residents of 20 villages were included in the study. Water samples were collected from 31 tube-wells located in the selected villages. Tomatoes, cabbages, and potatoes produced in the selected village were randomly sampled. Arsenic content in water, food, and residents' urine was determined by atomic absorption spectrophotometry using hydride generation method. Finally, 240 people were examined by a medical doctor for skin lesions. Arsenic concentrations from the tube-well water ranged from 1 to 124 μg/l. Arsenic concentrations of more than one-half (52%) of the water samples exceeded the WHO guideline value (10 μg/l). No trace of arsenic was found in the samples of tomatoes, cabbages, and potatoes. Variation in arsenic concentrations in the urines was correlated to arsenic concentrations in tube-well water. Clinical examinations revealed that melanosis and keratosis were respectively identified in 29.26% and 46.34% of the population. Both conditions were observed in 24.39% of the population. The frequency of skin lesions was positively associated with the arsenic concentration in tube-well water. A great majority (89.53%) of those who had skin lesions were at least 18 years old. In conclusion, chronic arsenic poisoning remains a major public health problem in the province of Yatenga (Burkina Faso).

Comparison of Poliovirus Detection in Water by Cell Culture and Nucleic Acid Hybridization

1993 ◽  
Vol 27 (3-4) ◽  
pp. 315-319
Author(s):  
Carlos E. Enriquez ◽  
Morteza Abbaszadegan ◽  
Ian L. Pepper ◽  
Kenneth J. Richardson ◽  
Aaron B. Margolin ◽  
...  
Keyword(s):  
Cell Culture ◽  
Tissue Culture ◽  
Nucleic Acid ◽  
Water Samples ◽  
Environmental Water ◽  
Nucleic Acid Hybridization ◽  
Well Water ◽  
Gene Probe ◽  
Hybridization Technique ◽  
Water Filter

The nucleic acid hybridization technique has been used to detect viral nucleic acid in environmental water samples. This type of assay, in contrast with tissue culture assays, may not distinguish between viable and non-viable viruses. We evaluated, by comparison with tissue culture infectivity assay (plaque forming method), the ability of the gene probe assay to detect viable poliovirus 1 (LSc) in well water, autoclaved well water, filter-sterilized well water and autoclaved phosphate buffered saline kept at 37° C and 15° C for 75 days, and in dechlorinated tapwater held at room temperature. A gradual decline in numbers of poliovirus was observed in all of the samples by cell culture assay. With the exception of autoclaved well water and phosphate buffer samples, a parallel decline in virus detectable by gene probe occurred in all other water samples.

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