scholarly journals Isolation and Identification of Legionella spp. in environmental water sources based on macrophage infectivity potentiator (mip) gene sequencing in southwest Iran

2019 ◽  
Vol 5 (3) ◽  
pp. 223-231
Author(s):  
Mojtaba Moosavian ◽  
◽  
Mina Moradzadeh ◽  
Ataollah Ghadiri ◽  
Morteza Saki ◽  
...  
Keyword(s):  
Gene Sequencing ◽  
Environmental Water ◽  
Water Sources ◽  
Isolation And Identification ◽  
Macrophage Infectivity Potentiator ◽  
Southwest Iran ◽  
Em Gene ◽  
Macrophage Infectivity

Inhibitors of macrophage infectivity potentiator-like PPIases affect neisserial and chlamydial pathogenicity

2016 ◽  
Vol 48 (4) ◽  
pp. 401-408 ◽  
Author(s):  
Anastasija Reimer ◽  
Florian Seufert ◽  
Matthias Weiwad ◽  
Jutta Ebert ◽  
Nicole M. Bzdyl ◽  
...  
Keyword(s):  
Macrophage Infectivity Potentiator ◽  
Macrophage Infectivity

scholarly journals Recombinant Protein Truncation Strategy for Inducing Bactericidal Antibodies to the Macrophage Infectivity Potentiator Protein of Neisseria meningitidis and Circumventing Potential Cross-Reactivity with Human FK506-Binding Proteins

2014 ◽  
Vol 83 (2) ◽  
pp. 730-742 ◽  
Author(s):  
Magdalena K. Bielecka ◽  
Nathalie Devos ◽  
Mélanie Gilbert ◽  
Miao-Chiu Hung ◽  
Vincent Weynants ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Bactericidal Activity ◽  
Binding Proteins ◽  
Leader Peptide ◽  
Type I ◽  
Globular Domain ◽  
His Tag ◽  
Macrophage Infectivity Potentiator ◽  
Fk506 Binding Proteins ◽  
Macrophage Infectivity

A recombinant macrophage infectivity potentiator (rMIP) protein ofNeisseria meningitidisinduces significant serum bactericidal antibody production in mice and is a candidate meningococcal vaccine antigen. However, bioinformatics analysis of MIP showed some amino acid sequence similarity to human FK506-binding proteins (FKBPs) in residues 166 to 252 located in the globular domain of the protein. To circumvent the potential concern over generating antibodies that could recognize human proteins, we immunized mice with recombinant truncated type I rMIP proteins that lacked the globular domain and the signal leader peptide (LP) signal sequence (amino acids 1 to 22) and contained the His purification tag at either the N or C terminus (C-term). The immunogenicity of truncated rMIP proteins was compared to that of full (i.e., full-length) rMIP proteins (containing the globular domain) with either an N- or C-terminal His tag and with or without the LP sequence. By comparing the functional murine antibody responses to these various constructs, we determined that C-term His truncated rMIP (−LP) delivered in liposomes induced high levels of antibodies that bound to the surface of wild-type but not Δmipmutant meningococci and showed bactericidal activity against homologous type I MIP (median titers of 128 to 256) and heterologous type II and III (median titers of 256 to 512) strains, thereby providing at least 82% serogroup B strain coverage. In contrast, in constructs lacking the LP, placement of the His tag at the N terminus appeared to abrogate bactericidal activity. The strategy used in this study would obviate any potential concerns regarding the use of MIP antigens for inclusion in bacterial vaccines.

scholarly journals Nocardia farcinica - a significant cause of mastitis in goats in Sudan : short communication

2004 ◽  
Vol 75 (3) ◽  
Author(s):  
L.A. Maldonado ◽  
M.E. Hamid ◽  
O.A. Gamal El Din ◽  
M. Goodfellow
Keyword(s):  
16S Rdna ◽  
Short Communication ◽  
Gene Sequencing ◽  
Isolation And Identification ◽  
16S Rdna Gene ◽  
Identification Methods ◽  
Milk Samples ◽  
Nocardia Farcinica ◽  
Mastitic Milk ◽  
16S Rdna Gene Sequencing

Fifteen of 100 mastitic milk samples from goats suffering from mastitis were tentatively identified as members of the genus Nocardia on the basis of selected phenotypic and chemotaxonomic characteristics. Six of the 15 strains were confirmed as Nocardia farcinica by 16S rDNA gene sequencing and subsequent aligning with relevant actinomycetes found in electronic databases and 2 by other identification criteria. N. farcinica is a serious cause of mastitis with a significant prevalence (15%) among the examined goats. Efforts are needed to optimise and simplify isolation and identification methods.

scholarly journals Recovery and Detection of Enteric Viruses from Non-Traditional Irrigation Water Sources

2019 ◽  
Vol 2 (3) ◽  
pp. 55 ◽  
Author(s):  
Brienna L. Anderson-Coughlin ◽  
Kalmia E. Kniel
Keyword(s):  
Irrigation Water ◽  
Water Samples ◽  
Hepatitis A Virus ◽  
Enteric Viruses ◽  
Environmental Water ◽  
Water Sources ◽  
Aichi Virus ◽  
Water Types ◽  
Virus Adsorption ◽  
Traditional Irrigation

The variability of environmental water samples impacts the allowance of one method to be universally ideal for all water types and volumes. Surface and reclaimed waters can be used for crop irrigation and may be referred to as non-traditional irrigation waters as these water types may be associated with a higher risk of microbial contamination compared to groundwater. These waters are typically more microbially and chemically complex than groundwater and have a higher risk of viral contamination. To detect viruses in these water types, an infinite number of variations can be made to traditional recovery methods. This protocol was developed based on a commonly used virus adsorption and elution (VIRADEL) method. Additional steps were included to simplify and efficiently reduce particulates in the viral concentrate and remove DNA from eluted nucleic acids prior to detection. Method alterations allow for volumes up to 40 liters to be processed with consistent recovery of enteric viruses including Aichi virus, hepatitis A virus, and noroviruses belonging to genogroups GI and GII. No inhibition was observed among either surface or reclaimed water samples. This protocol could be utilized in the monitoring of a wide array of irrigation water sources throughout irrigation processes.

A fluorescent three-sensor array for heavy metals in environmental water sources

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1195-1201 ◽  
Author(s):  
Amy A. Bowyer ◽  
Clara Shen ◽  
Elizabeth J. New
Keyword(s):  
Heavy Metals ◽  
Sensor Array ◽  
Environmental Water ◽  
Water Sources ◽  
Toxic Heavy Metals ◽  
Fluorescent Sensing ◽  
Calcein Blue

A fluorescent sensing array based on analogues of Calcein Blue is able to classify toxic heavy metals in water.

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